DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ yu-2015-1 in vitro In vitro glycosylase assay glycosylase assay predetermined predetermined participant predetermined Yu H., Takeuchi M., LeBarron J., Kantharia J., London E., Bakker H., Haltiwanger RS., Li H., Takeuchi H. DIP original experiment: DIP-268146X Nature chemical biology 2015 IMEx Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Yu H., Takeuchi M., LeBarron J., Kantharia J., London E., Bakker H., Haltiwanger RS., Li H., Takeuchi H. Only protein-protein interactions imex curation yu-2015-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight silver stain molecular weight estimation by silver staining weight silver stain Yu H., Takeuchi M., LeBarron J., Kantharia J., London E., Bakker H., Haltiwanger RS., Li H., Takeuchi H. DIP original experiment: DIP-268144X Nature chemical biology 2015 IMEx Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Yu H., Takeuchi M., LeBarron J., Kantharia J., London E., Bakker H., Haltiwanger RS., Li H., Takeuchi H. Only protein-protein interactions imex curation yu-2015-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Yu H., Takeuchi M., LeBarron J., Kantharia J., London E., Bakker H., Haltiwanger RS., Li H., Takeuchi H. DIP original experiment: DIP-268145X Nature chemical biology 2015 IMEx Notch-modifying xylosyltransferase structures support an SNi-like retaining mechanism. Yu H., Takeuchi M., LeBarron J., Kantharia J., London E., Bakker H., Haltiwanger RS., Li H., Takeuchi H. Only protein-protein interactions imex curation fa9_human Coagulation factor IX F9 Christmas factor Plasma thromboplastin component protein protein human Homo sapiens Human MQRVNMIMAESPGLITICLLGYLLSAECTVFLDHENANKILNRPKRYNSGKLEEFVQGNLERECMEEKCSFEEAREVFENTERTTEFWKQYVDGDQCESNPCLNGGSCKDDINSYECWCPFGFEGKNCELDVTCNIKNGRCEQFCKNSADNKVVCSCTEGYRLAENQKSCEPAVPFPCGRVSVSQTSKLTRAETVFPDVDYVNSTEAETILDNITQSTQSFNDFTRVVGGEDAKPGQFPWQVVLNGKVDAFCGGSIVNEKWIVTAAHCVETGVKITVVAGEHNIEETEHTEQKRNVIRIIPHHNYNAAINKYNHDIALLELDEPLVLNSYVTPICIADKEYTNIFLKFGSGYVSGWGRVFHKGRSALVLQYLRVPLVDRATCLRSTKFTIYNNMFCAGFHEGGRDSCQGDSGGPHVTEVEGTSFLTGIISWGEECAMKGKYGIYTKVSRYVNWIKEKTKLT xxlt1_mouse Xyloside xylosyltransferase 1 Xxylt1 UDP-xylose:alpha-xyloside alpha-1,3-xylosyltransferase protein protein mouse Mus musculus Mouse MGLLRGGAACARAMARLGALRSHYCALLLAAALAVCAFYYLGSGRETFSSATKRLKEARAGAAAPTPPAPELARGSAAPASGAKAKSLEGGVVVPVDYHLLMMFTKAEHNAPLQAKARVALSSLLRLAKFEAHEVLNLHFVSEEASREVAKALLRELLPPAAGFKCKVIFHDVAVLTDKLFPVVEAMQKYFSAGSGTYYSDSIFFLSVAMHQIMPKEIPRIIQLDLDLKYKTNIRELFEEFDNFLPGAVIGIAREMQPVYRHTFWQFRHENPKTRVGDPPPEGLPGFNSGVMLLNLEAMRQSPLYSHLLEPSWVQQLADKYHFRGHLGDQDFFTMIGMEHPELFHVLDCTWNRQLCTWWRDHGYSDVFQAYFRCEGHVKIYHGNCNTPIPED f9-xxylt1-1 3284341 N/A 3284344 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 92-130 Region 92-130 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3284345 enzyme enzyme unspecified role unspecified role Region 87-392 Region 87-392 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q3U4G3:p.Gln330Ala Q330A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q3U4G3:p.Trp359Ala W359A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q3U4G3:p.Glu255Ala E255A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q3U4G3:p.His326Ala H326A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q3U4G3:p.Trp358Ala W358A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q3U4G3:p.Asn384Ala N384A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q3U4G3:p.Gln257Ala Q257A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q3U4G3:p.Ser289Ala S289A mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q3U4G3:p.Arg324Ser R324S mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q3U4G3:p.Gly325Ser G325S mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human glycosylation glycosylation reaction glycosylation false false false f9-xxylt1-2 3284342 N/A 3284344 unspecified role unspecified role unspecified role unspecified role Region 92-130 Region 92-130 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3284345 unspecified role unspecified role unspecified role unspecified role Region 87-392 Region 87-392 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false f9-xxylt1-3 3284343 N/A 3284344 unspecified role unspecified role unspecified role unspecified role Region 92-130 Region 92-130 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3284345 unspecified role unspecified role unspecified role unspecified role Region 87-392 Region 87-392 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false