DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ge-2015-5 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Ge J., Li W., Zhao Q., Li N., Chen M., Zhi P., Li R., Gao N., Xiao B., Yang M. DIP original experiment: DIP-267160X Nature 2015 IMEx Architecture of the mammalian mechanosensitive Piezo1 channel. Ge J., Li W., Zhao Q., Li N., Chen M., Zhi P., Li R., Gao N., Xiao B., Yang M. Only protein-protein interactions imex curation ge-2015-6 human Homo sapiens Human molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Ge J., Li W., Zhao Q., Li N., Chen M., Zhi P., Li R., Gao N., Xiao B., Yang M. DIP original experiment: DIP-267156X Nature 2015 IMEx Architecture of the mammalian mechanosensitive Piezo1 channel. Ge J., Li W., Zhao Q., Li N., Chen M., Zhi P., Li R., Gao N., Xiao B., Yang M. Only protein-protein interactions imex curation ge-2015-7 human Homo sapiens Human comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel western blot western blot Immuno blot Immuno blot Ge J., Li W., Zhao Q., Li N., Chen M., Zhi P., Li R., Gao N., Xiao B., Yang M. DIP original experiment: DIP-267157X Nature 2015 IMEx Architecture of the mammalian mechanosensitive Piezo1 channel. Ge J., Li W., Zhao Q., Li N., Chen M., Zhi P., Li R., Gao N., Xiao B., Yang M. Only protein-protein interactions imex curation ge-2015-8 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ge J., Li W., Zhao Q., Li N., Chen M., Zhi P., Li R., Gao N., Xiao B., Yang M. DIP original experiment: DIP-267161X Nature 2015 IMEx Architecture of the mammalian mechanosensitive Piezo1 channel. Ge J., Li W., Zhao Q., Li N., Chen M., Zhi P., Li R., Gao N., Xiao B., Yang M. Only protein-protein interactions imex curation ge-2015-9 human Homo sapiens Human tem transmission electron microscopy tem predetermined predetermined participant predetermined Ge J., Li W., Zhao Q., Li N., Chen M., Zhi P., Li R., Gao N., Xiao B., Yang M. DIP original experiment: DIP-267158X Nature 2015 IMEx Architecture of the mammalian mechanosensitive Piezo1 channel. Ge J., Li W., Zhao Q., Li N., Chen M., Zhi P., Li R., Gao N., Xiao B., Yang M. Only protein-protein interactions imex curation ge-2015-10 human Homo sapiens Human 3d-em 3D electron microscopy 3D-EM electron tomog predetermined predetermined participant predetermined Ge J., Li W., Zhao Q., Li N., Chen M., Zhi P., Li R., Gao N., Xiao B., Yang M. DIP original experiment: DIP-267159X Nature 2015 IMEx Architecture of the mammalian mechanosensitive Piezo1 channel. Ge J., Li W., Zhao Q., Li N., Chen M., Zhi P., Li R., Gao N., Xiao B., Yang M. Only protein-protein interactions imex curation piez1_mouse Piezo-type mechanosensitive ion channel component 1 Piezo1 Fam38a Protein FAM38A protein protein mouse Mus musculus Mouse MEPHVLGAGLYWLLLPCTLLAASLLRFNALSLVYLLFLLLLPWLPGPSRHSIPGHTGRLLRALLCLSLLFLVAHLAFQICLHTVPHLDQFLGQNGSLWVKVSQHIGVTRLDLKDIFNTTRLVAPDLGVLLASSLCLGLCGRLTRKAGQSRRTQELQDDDDDDDDDDEDIDAAPAVGLKGAPALATKRRLWLASRFRVTAHWLLMTSGRTLVIVLLALAGIAHPSAFSSIYLVVFLAICTWWSCHFPLSPLGFNTLCVMVSCFGAGHLICLYCYQTPFIQDMLPPGNIWARLFGLKNFVDLPNYSSPNALVLNTKHAWPIYVSPGILLLLYYTATSLLKLHKSCPSELRKETPREDEEHELELDHLEPEPQARDATQGEMPMTTEPDLDNCTVHVLTSQSPVRQRPVRPRLAELKEMSPLHGLGHLIMDQSYVCALIAMMVWSIMYHSWLTFVLLLWACLIWTVRSRHQLAMLCSPCILLYGLTLCCLRYVWAMELPELPTTLGPVSLHQLGLEHTRYPCLDLGAMLLYLLTFWLLLRQFVKEKLLKKQKVPAALLEVTVADTEPTQTQTLLRSLGELVTGIYVKYWIYVCAGMFIVVSFAGRLVVYKIVYMFLFLLCLTLFQVYYTLWRKLLRVFWWLVVAYTMLVLIAVYTFQFQDFPTYWRNLTGFTDEQLGDLGLEQFSVSELFSSILIPGFFLLACILQLHYFHRPFMQLTDLEHVPPPGTRHPRWAHRQDAVSEAPLLEHQEEEEVFREDGQSMDGPHQATQVPEGTASKWGLVADRLLDLAASFSAVLTRIQVFVRRLLELHVFKLVALYTVWVALKEVSVMNLLLVVLWAFALPYPRFRPMASCLSTVWTCIIIVCKMLYQLKIVNPHEYSSNCTEPFPNNTNLQPLEINQSLLYRGPVDPANWFGVRKGYPNLGYIQNHLQILLLLVFEAVVYRRQEHYRRQHQQAPLPAQAVCADGTRQRLDQDLLSCLKYFINFFFYKFGLEICFLMAVNVIGQRMNFMVILHGCWLVAILTRRRREAIARLWPNYCLFLTLFLLYQYLLCLGMPPALCIDYPWRWSKAIPMNSALIKWLYLPDFFRAPNSTNLISDFLLLLCASQQWQVFSAERTEEWQRMAGINTDHLEPLRGEPNPIPNFIHCRSYLDMLKVAVFRYLFWLVLVVVFVAGATRISIFGLGYLLACFYLLLFGTTLLQKDTRAQLVLWDCLILYNVTVIISKNMLSLLSCVFVEQMQSNFCWVIQLFSLVCTVKGYYDPKEMMTRDRDCLLPVEEAGIIWDSICFFFLLLQRRIFLSHYFLHVSADLKATALQASRGFALYNAANLKSINFHRQIEEKSLAQLKRQMKRIRAKQEKYRQSQASRGQLQSKDPQDPSQEPGPDSPGGSSPPRRQWWRPWLDHATVIHSGDYFLFESDSEEEEEALPEDPRPAAQSAFQMAYQAWVTNAQTVLRQRRERARQERAEQLASGGDLNPDVEPVDVPEDEMAGRSHMMQRVLSTMQFLWVLGQATVDGLTRWLRAFTKHHRTMSDVLCAERYLLTQELLRVGEVRRGVLDQLYVGEDEATLSGPVETRDGPSTASSGLGAEEPLSSMTDDTSSPLSTGYNTRSGSEEIVTDAGDLQAGTSLHGSQELLANARTRMRTASELLLDRRLHIPELEEAERFEAQQGRTLRLLRAGYQCVAAHSELLCYFIIILNHMVTASAASLVLPVLVFLWAMLTIPRPSKRFWMTAIVFTEVMVVTKYLFQFGFFPWNSYVVLRRYENKPYFPPRILGLEKTDSYIKYDLVQLMALFFHRSQLLCYGLWDHEEDRYPKDHCRSSVKDREAKEEPEAKLESQSETGTGHPKEPVLAGTPRDHIQGKGSIRSKDVIQDPPEDLKPRHTRHISIRFRRRKETPGPKGTAVMETEHEEGEGKETTERKRPRHTQEKSKFRERMKAAGRRLQSFCVSLAQSFYQPLQRFFHDILHTKYRAATDVYALMFLADIVDIIIIIFGFWAFGKHSAATDIASSLSDDQVPQAFLFMLLVQFGTMVIDRALYLRKTVLGKLAFQVVLVVAIHIWMFFILPAVTERMFSQNAVAQLWYFVKCIYFALSAYQIRCGYPTRILGNFLTKKYNHLNLFLFQGFRLVPFLVELRAVMDWVWTDTTLSLSNWMCVEDIYANIFIIKCSRETEKKYPQPKGQKKKKIVKYGMGGLIILFLIAIIWFPLLFMSLIRSVVGVVNQPIDVTVTLKLGGYEPLFTMSAQQPSIVPFTPQAYEELSQQFDPYPLAMQFISQYSPEDIVTAQIEGSSGALWRISPPSRAQMKQELYNGTADITLRFTWNFQRDLAKGGTVEYTNEKHTLELAPNSTARRQLAQLLEGRPDQSVVIPHLFPKYIRAPNGPEANPVKQLQPDEEEDYLGVRIQLRREQVGTGASGEQAGTKASDFLEWWVIELQDCKADCNLLPMVIFSDKVSPPSLGFLAGYGIVGLYVSIVLVVGKFVRGFFSEISHSIMFEELPCVDRILKLCQDIFLVRETRELELEEELYAKLIFLYRSPETMIKWTRERE piezo1-1 3284294 N/A 3284300 unspecified role unspecified role unspecified role unspecified role Region 2214-2457 Region 2214-2457 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false piezo1-2 3284295 N/A 3284300 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human direct interaction direct interaction false false false piezo1-3 3284296 N/A 3284300 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human direct interaction direct interaction false false false piezo1-4 3284297 N/A 3284300 unspecified role unspecified role unspecified role unspecified role Region 2214-2457 Region 2214-2457 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false piezo1-5 3284298 N/A 3284300 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human direct interaction direct interaction false false false piezo1-6 3284299 N/A 3284300 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human direct interaction direct interaction false false false