DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ jadhav-2015-1 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Jadhav B., Wild K., Pool MR., Sinning I. DIP original experiment: DIP-267263X Structure (London, England : 1993) 2015 IMEx Structure and Switch Cycle of SRβ as Ancestral Eukaryotic GTPase Associated with Secretory Membranes. Jadhav B., Wild K., Pool MR., Sinning I. Only protein-protein interactions imex curation jadhav-2015-2 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Jadhav B., Wild K., Pool MR., Sinning I. DIP original experiment: DIP-267261X Structure (London, England : 1993) 2015 IMEx Structure and Switch Cycle of SRβ as Ancestral Eukaryotic GTPase Associated with Secretory Membranes. Jadhav B., Wild K., Pool MR., Sinning I. Only protein-protein interactions imex curation jadhav-2015-3 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jadhav B., Wild K., Pool MR., Sinning I. DIP original experiment: DIP-267262X Structure (London, England : 1993) 2015 IMEx Structure and Switch Cycle of SRβ as Ancestral Eukaryotic GTPase Associated with Secretory Membranes. Jadhav B., Wild K., Pool MR., Sinning I. Only protein-protein interactions imex curation jadhav-2015-4 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati weight by comassie molecular weight estimation by coomasie staining weight by comassie Jadhav B., Wild K., Pool MR., Sinning I. DIP original experiment: DIP-267264X Structure (London, England : 1993) 2015 IMEx Structure and Switch Cycle of SRβ as Ancestral Eukaryotic GTPase Associated with Secretory Membranes. Jadhav B., Wild K., Pool MR., Sinning I. Only protein-protein interactions imex curation g0s401_chatd Signal recognition particle receptor subunit beta CTHT_0022040 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MITLEDLKNFFEMALKPSPLVFKIGFAIVLLVPFILHLIFSATTQYTTLPSVLLIGPSGAGKTALLTLFERGPLLNPDGTSVGAADLKNPYRKPIVTSPVAQTHTSQVPTSVELAVGANEDGTPTSYKVDLDAAGATARKFLLIDTPGHPKLRGTTLQHLLNPSPSLTIIPTNAPNKKTSTDSHSDPYKSKLKAVIFLLDAAALADSDGDYLSQTASYLYDVLLSLQKRFHSRKNSRAPSSIPVLIAANKQDLFTAVPASLVKSRLEHELGRIRKTRQKGLLEASVTSEDEIRADDEEGWLGAVGSKEFKFEEMMEFDMEVEVMGGNVIGDGPGAERWWRWIGERI g0s1r8_chatd Non-specific serine/threonine protein kinase CTHT_0014570 protein protein chatd Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719) MLDAFEILTTSGVVLWSRTYAPVNPSVVNDFITDVFIEEKSAVAGSKNGGSAASNPPYKHDQHSLRWTFVKELGIIFVAVYRSLLHLPWVDKLVDNIRAIFVSLYSEQFKRPNTTIIECINFDKYFDQQLQELEQTGSRVDARVPKIEAHSADEEEQPFVPSPAGKSEQKAPIESISQNHSRPTTSSTSHLVVPRPGPGGKLSRRARKLQNSNSAPASSGDEAPSRKGKQGKLVKRGRKWDADGLAIEDDGSDVQLDYSAPALTSDSEAEASGRAISIERVDASTMGSRTKGKFVLKDLGDEVHSILADAEAKKNAETSSEASSGIVGSSLNAIGGLFRNFVGGKVLTKQDLEKAMKGMEEHLLKKNVAREAAVRLCESVEKELIGVKTGSFESISRTVQKAMEASLTKMLTPTSSIDLLREIDSVTSPPPTSGRQRRPYVISIVGVNGVGKSTNLSKICFFLLQNQYKVLIAAADTFRSGAVEQLAVHVRNLKELTAREGGRVELYQKGYGKDAAAVAKDAVVFAAQEGFDVVLIDTAGRRHNDQRLMSSLEKFTKLAKPDKILMVGEALVGTDSVAQARNFNAAFGAGRSLDGFIISKCDTVGDMVGTLVSIPGQHSYADSAGPAGGPLPSAAPPPSTLAAQLVENISTSSTRLTRPDETSELKRLFAAIEKVKNDPSCLKTPEQRLEHNHLLVYVCGSVLLDNLKLDDAFADRERLCSETSRAINFLKVTIQETPAVLKCTTDGRTFIHRGQEPLWVWILPKVLRVLGHRRFISLSTEIKNFCCEVLKLAMGNEKLWDLAPLLLIYLRTNLGAILARYKSSDMTNLQHGPSAITLPPNVFFETWNGSAPHTCTFTIPDGEHALRHAVTLATIIADVSISRTHEVQQISPHDQHFCQASGYLAAEDEDGASLRRVFCLALAHLAKASTNHEPTARLVTSVLLPLAQRLTADNPALGASTDVWRSVQLLVRTVAGSTSPELSEEIAADKFIDTQLRRQVQKLPLRRTREQLELRPAKRKRSDKGPSLMLDVAGRLCRLVGADPSSQVDALESHIMERYPQLNEDDQCYLIDLVSRIPCVADETLELRKRSLSAGRHQEVKHLGITLFSKIIKLPRFIESKKPRVYAMICLRRIARHSTDEDFWDLEKSDPGQWCLQSLNSSIRELRIAAGRAIPVFITEPALATINKEVIKRNRTTVLAFLRSLSEKESPRLHETCIMAWAQVGRVVADDELNLVVLKLVEYLGHRNLTVSSFAFNEIINLAESRGQSPSQLFEPFWSNLGFAVVKDLMTRPQTARMVADLLQLSVPELLELLQKHALPWLVLTKKREVIRKIAEARGEKEEWQPCLDQGNLPSILALLLIQDTPDVATHAMSLLGHVSEKIANSELVELLRTDPITIALELFKAAADSNENRKTRARDQNDKRAKKSQVIGRYLQQHALGLAQRLIEVINDTLQLHPPVAEQRRCLGAMEEMIRICKTYVCIARPQISACLLSAMALDDLRSAAFSCWEAMLTCMEEADVEAQLETTFFIIGSYWKTFDLATKQKVKSLISTLLEAHQALLQKYANRLPSLRHISELSDLCTALDNLRPPLDVRGAFGIFAERLKHENPGVVELALVELNAFLQKHQDYVQTSAISEQPDTVVTALVRSLLDCSAKYNGWQTDIPRLCAEAMGLIGCLDSNRVETTREQRQFVVVHNFEDARETTDFVAYMLENVLVKAFLSTTDTKFIGFLSYAMQELLERTDFKYAAADPKSEIHAAIYKKWLAFSDNTREVLTPFLSSRFVVAPMPPQTIEYPVFKPEKPYAVWVRAFVLDLLRNGQHPFSQALFEPLCRLIKVKDLVVTEFLLPYVVLHVIVGQDHTDAFRKKITAELTAILQHQPPETASYVEKEDAKLYYQAVFRIIDYSRRWLQVKKSNHKVSDRDQSWIKRVEEVIGSLDPELMSQRAIDCNEYARALFFLEPHIEARGKHLTHERGDRMLQSLQNIYTNIDDPDGLEGVSAILQSITLDQQALNHRKAGRWTAAQTWYEIRLAESPDDPDIQQDLLTCLKESGQHDVLLNYVEGMNVTESNVTKVAPFAVEAAWATGRWQTLEKYLRLYNAGDVSEIFNLGVGQALLCLKSGDVAKFKEHIQMLRDKVAGSMTYSATSSLRACHDAMLKCHVLSDLELIANENLKGNGDQQAVLATLNRRLEVLGAYVSDKQYLLGIRRAAMELMRPRFSNEDISSQWLASGKLARKAGSMHQSFNAVLHAQQLGDGSATIEHAKLLYKDGHHRKAIQILQSAIATNSFITDNSALTLPPTSSKGQETQRSLLTARAHLLLAKWLDSTGQTHASALRSQYQQAAKTYPHWEKGHYYLGRHYKKLLESEKALKPDDQSDEYLTGEMAKLVIENYLRSLNFGTKYLSQTLPRILTLFLELGTQVENIRTSESKITVTRELHQRRKNILMDLYKHFLKHIPKMPAYIFYTALPQIVARIAHPNQEVFKVLEQMILKVIEAHPQQALWSLFPLMTTRYSHERRTRGMQILQAARSLSKRVEGADYDLSKLIRMGEKLAEQLLVACNTGEFQSNKTITASITKDLNFNHKCTPCPLVVPIEACLVATLPTLTDNVRKHKAFSRDVITIHSFLDEVLILGSLAKPRKLTARGSDGKLYGLLIKPKDDLRTDQRLMEFNGLINRSLKRDAEASRRQLYIRTYAVTPLNEECGIIEWVSGLKPLRDILLALYRARGISPNYGELSNKMRHSMMTGDIRIWTDDIIPSFPPVLPEWFIQQFPNPSDWFAARLKYTRSCAVMSMVGTILGLGDRHGENVLLEEGNGGIFHVDFNCLFDKGKTFAQPERVPFRLTHNMQAAMGIYRHEGPFRHCSELTLRILRQQEETLMTILEAFIYDPTLDLQRTKRRQHDGGVIRMDPNSVVETIRRKVRGLLPDESIPLGVEGQVEELIKEATNPKNLVAMYIGWCPFL g0s401_chatd-g0s1r8_chatd-1 3282764 N/A 3282768 unspecified role unspecified role bait bait Region 42-346 Region 42-346 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3282769 unspecified role unspecified role prey prey Region 1-172 Region 1-172 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false g0s1r8_chatd-g0s401_chatd-1 3282765 N/A 3282768 unspecified role unspecified role unspecified role unspecified role Region 42-346 Region 42-346 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282769 unspecified role unspecified role unspecified role unspecified role Region 1-172 Region 1-172 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false g0s1r8_chatd-g0s401_chatd-2 3282766 N/A 3282768 unspecified role unspecified role unspecified role unspecified role Region 42-346 Region 42-346 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282769 unspecified role unspecified role unspecified role unspecified role Region 1-172 Region 1-172 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false g0s1r8_chatd-g0s401_chatd-3 3282767 N/A 3282768 unspecified role unspecified role unspecified role unspecified role Region 42-346 Region 42-346 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3282769 unspecified role unspecified role unspecified role unspecified role Region 1-172 Region 1-172 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false