IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ trsch-2015-2 arath-protoplast Arabidopsis thaliana protoplast Protoplast defined as a membrane-bound cell from which the outer wall has been partially or completely removed. fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret tag fluorescence tag visualisation by fluorescence tag fluorescence Trösch R., Töpel M., Flores-Pérez Ú., Jarvis P. Vectors pSAT4(A)-nEYFP-N1 and pSAT4(A)-cEYFP-N1: EYGP fragments were deduced to be fused to the C terminus of any protein. (Tzfira et al., 2005) pmid:15821977 A UniProt identifier for ALB4 was inferred from later work published by this group; pmid:28684427 paul.jarvis@plants.ox.ac.uk Plant physiology 2015 Only protein-protein interactions imex curation imex curation trsch-2015-1 arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress crosslink cross-linking study crosslink western blot western blot Immuno blot Immuno blot Trösch R., Töpel M., Flores-Pérez Ú., Jarvis P. Cross-linker dithiobis succinimidyl propionate (DSP) was used followed by anti-flag or anti-ALB4 IP. A UniProt identifier for ALB4 was inferred from later work published by this group; pmid:28684427 paul.jarvis@plants.ox.ac.uk Plant physiology 2015 Only protein-protein interactions imex curation imex curation alb3_arath Inner membrane protein ALBINO3, chloroplastic ALB3 F8N16.9 At2g28800 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MARVLVSSPSSFFGSPLIKPSSSLRHSGVGGGGTAQFLPYRSNNNKLFTTSTTVRFSLNEIPPFHGLDSSVDIGAIFTRAESLLYTIADAAVVGADSVVTTDSSAVQKSGGWFGFISDAMELVLKILKDGLSAVHVPYAYGFAIILLTIIVKAATYPLTKQQVESTLAMQNLQPKIKAIQQRYAGNQERIQLETSRLYKQAGVNPLAGCLPTLATIPVWIGLYQALSNVANEGLFTEGFFWIPSLGGPTSIAARQSGSGISWLFPFVDGHPPLGWYDTVAYLVLPVLLIASQYVSMEIMKPPQTDDPAQKNTLLVFKFLPLMIGYFALSVPSGLSIYWLTNNVLSTAQQVYLRKLGGAKPNMDENASKIISAGRAKRSIAQPDDAGERFRQLKEQEKRSKKNKAVAKDTVELVEESQSESEEGSDDEEEEAREGALASSTTSKPLPEVGQRRSKRSKRKRTV alb4_arath ALBINO3-like protein 1, chloroplastic ALB4 ALB ALB3L1 ART1 Arabidopsis thaliana envelope membrane integrase Protein ALBINA 4 Protein ARTEMIS STIC1 Suppressor of tic40 protein 1 F21J9.16 F21J9_170 At1g24490 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSSTISLKPTHLILSSFSTGKVLQFRRSRFSHTPSSSSSRYRTLVAQLGFRPDSFDFIKDHAENLLYTIADAAVSSSETFESVAGTTTKTTQSNDWFSGIANYMETILKVLKDGLSTVHVPYSYGFAIILLTVLVKAATFPLTKKQVESAMAMKSLTPQIKAIQERYAGDQEKIQLETARLYKLAGINPLAGCLPTLATIPVWIGLYRALSNVADEGLLTEGFFWIPSLAGPTTVAARQNGSGISWLFPFIEGHPPLGWPDTLAYLVLPLLLVFSQYLSIQIMQSSQSNDPAMKSSQAVTKLLPLMIGYFALSVPSGLSLYWLTNNILSTAQQVWLQKYGGAKNPVEKFTNLVTKEDKTQQIEKSFSEPLVQKSVSELKIPREKGGEKVTPECPKPGERFRLLKEQEAKRRREKEERQKAEAALSNQNTDKAHEQDEKSDTAIVAEDDKKTELSAVDETSDGTVAVNGKPSIQKDETTNGTFGIGHDTEQQHSHETEKR vipp1_arath Membrane-associated protein VIPP1, chloroplastic VIPP1 Protein VESICLE-INDUCING PROTEIN IN PLASTIDS 1 T8F5.2 At1g65260 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MALKASPVTGLFPPLRPTASSSPSTSSNRPCSLRILPLRTSFFGNSSGALRVNVLRLACDNRLRCNGHGATMNLFERFSRVVKSYANALISSFEDPEKILEQTVIEMNSDLTKMRQATAQVLASQKQLQNKYKAAQQSSDDWYKRAQLALAKGDEDLAREALKRRKSFADNATALKTQLDQQKGVVDNLVSNTRLLESKIQEAKAKKDTLLARARTAKTATKVQEMIGTVNTSGALSAFEKMEEKVMAMESEADALTQIGTDELEGKFQMLETSSVDDDLADLKKELSGSSKKGELPPGRSTVAASTRYPFKDSEIENELNELRRKANDF hsp7f_arath Heat shock 70 kDa protein 6, chloroplastic HSP70-6 CPHSC70-1 Chloroplast heat shock protein 70-1 Heat shock protein 70-6 T22A6.110 At4g24280 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MASSAAQIHVLGGIGFASSSSSKRNLNGKGGTFMPRSAFFGTRTGPFSTPTSAFLRMGTRNGGGASRYAVGPVRVVNEKVVGIDLGTTNSAVAAMEGGKPTIVTNAEGQRTTPSVVAYTKSGDRLVGQIAKRQAVVNPENTFFSVKRFIGRKMNEVDEESKQVSYRVVRDENNNVKLECPAINKQFAAEEISAQVLRKLVDDASRFLNDKVTKAVITVPAYFNDSQRTATKDAGRIAGLEVLRIINEPTAASLAYGFDRKANETILVFDLGGGTFDVSVLEVGDGVFEVLSTSGDTHLGGDDFDKRVVDWLAAEFKKDEGIDLLKDKQALQRLTEAAEKAKIELSSLTQTNMSLPFITATADGPKHIETTLTRAKFEELCSDLLDRVRTPVENSLRDAKLSFKDIDEVILVGGSTRIPAVQELVRKVTGKEPNVTVNPDEVVALGAAVQAGVLAGDVSDIVLLDVTPLSIGLETLGGVMTKIIPRNTTLPTSKSEVFSTAADGQTSVEINVLQGEREFVRDNKSLGSFRLDGIPPAPRGVPQIEVKFDIDANGILSVSAVDKGTGKKQDITITGASTLPKDEVDQMVQEAERFAKDDKEKRDAIDTKNQADSVVYQTEKQLKELGEKIPGEVKEKVEAKLQELKDKIGSGSTQEIKDAMAALNQEVMQIGQSLYNQPGAGGPGAGPSPGGEGASSGDSSSSKGGDGDDVIDADFTDSQ sr54c_arath Signal recognition particle 54 kDa protein, chloroplastic FFC At5g03940 F8F6_150 FFC CPSRP54 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEALQFSSVNRVPCTLSCTGNRRIKAAFSSAFTGGTINSASLSSSRNLSTREIWSWVKSKTVVGHGRYRRSQVRAEMFGQLTGGLEAAWSKLKGEEVLTKDNIAEPMRDIRRALLEADVSLPVVRRFVQSVSDQAVGMGVIRGVKPDQQLVKIVHDELVKLMGGEVSELQFAKSGPTVILLAGLQGVGKTTVCAKLACYLKKQGKSCMLIAGDVYRPAAIDQLVILGEQVGVPVYTAGTDVKPADIAKQGLKEAKKNNVDVVIMDTAGRLQIDKGMMDELKDVKKFLNPTEVLLVVDAMTGQEAAALVTTFNVEIGITGAILTKLDGDSRGGAALSVKEVSGKPIKLVGRGERMEDLEPFYPDRMAGRILGMGDVLSFVEKATEVMRQEDAEDLQKKIMSAKFDFNDFLKQTRAVAKMGSMTRVLGMIPGMGKVSPAQIREAEKNLLVMEAMIEVMTPEERERPELLAESPERRKRIAKDSGKTEQQVSALVAQIFQMRVKMKNLMGVMEGGSIPALSGLEDALKAEQKAPPGTARRKRKADSRKKFVESASSKPGPRGFGSGN sr43c_arath Signal recognition particle 43 kDa protein, chloroplastic CAO T30B22.25 At2g47450 Chromo protein SRP43 CPSRP43 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MQKVFLAMDTCALVIHQSLSRIKLSPPKSSSSSSSAFSPESLPIRRIELCFRGAICAAVQRNYEETTSSVEEAEEDDESSSSYGEVNKIIGSRTAGEGAMEYLIEWKDGHSPSWVPSSYIAADVVSEYETPWWTAARKADEQALSQLLEDRDVDAVDENGRTALLFVAGLGSDKCVRLLAEAGADLDHRDMRGGLTALHMAAGYVRPEVVEALVELGADIEVEDERGLTALELAREILKTTPKGNPMQFGRRIGLEKVINVLEGQVFEYAEVDEIVEKRGKGKDVEYLVRWKDGGDCEWVKGVHVAEDVAKDYEDGLEYAVAESVIGKRVGDDGKTIEYLVKWTDMSDATWEPQDNVDSTLVLLYQQQQPMNE alb3-ebfc2_arath-2 3318045 N/A 3318047 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3318048 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus cyfp cYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false proximity proximity close-contact colocalization neighbourhood interaction false false false 6-4 alb3-ebfc2_arath-3 3318045 N/A 3318047 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3318047 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus cyfp cYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false proximity proximity close-contact colocalization neighbourhood interaction false false false 6-1 alb3l1-ebfc2_arath-3 3318045 N/A 3318048 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3318048 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus cyfp cYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false proximity proximity close-contact colocalization neighbourhood interaction false false false 6-2 alb3-alb3l1-6 3318045 N/A 3318047 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus cyfp cYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3318048 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed living cell living cell c_terminus nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false proximity proximity close-contact colocalization neighbourhood interaction false false false 6-3 alb3-alb3l1-4 3318046 N/A 3318048 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3318047 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false 5A alb3-alb3l1-5 3318046 N/A 3318048 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3318047 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association false false false 5B alb3-alb3l1-7 3318046 N/A 3318049 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3318050 unspecified role unspecified role prey prey N/A 3318051 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3318052 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level N/A 3318048 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false 7