DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ranaivoson-2015-1 in vitro In vitro h1-h2 ms mass spectrometry study of hydrogen/deuterium exchange h1-h2 ms predetermined predetermined participant predetermined Ranaivoson FM., Liu Q., Martini F., Bergami F., von Daake S., Li S., Lee D., Demeler B., Hendrickson WA., Comoletti D. DIP original experiment: DIP-267241X Structure (London, England : 1993) 2015 IMEx Structural and Mechanistic Insights into the Latrophilin3-FLRT3 Complex that Mediates Glutamatergic Synapse Development. Ranaivoson FM., Liu Q., Martini F., Bergami F., von Daake S., Li S., Lee D., Demeler B., Hendrickson WA., Comoletti D. Only protein-protein interactions imex curation ranaivoson-2015-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Ranaivoson FM., Liu Q., Martini F., Bergami F., von Daake S., Li S., Lee D., Demeler B., Hendrickson WA., Comoletti D. DIP original experiment: DIP-267237X Structure (London, England : 1993) 2015 IMEx Structural and Mechanistic Insights into the Latrophilin3-FLRT3 Complex that Mediates Glutamatergic Synapse Development. Ranaivoson FM., Liu Q., Martini F., Bergami F., von Daake S., Li S., Lee D., Demeler B., Hendrickson WA., Comoletti D. Only protein-protein interactions imex curation ranaivoson-2015-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ranaivoson FM., Liu Q., Martini F., Bergami F., von Daake S., Li S., Lee D., Demeler B., Hendrickson WA., Comoletti D. DIP original experiment: DIP-267235X Structure (London, England : 1993) 2015 IMEx Structural and Mechanistic Insights into the Latrophilin3-FLRT3 Complex that Mediates Glutamatergic Synapse Development. Ranaivoson FM., Liu Q., Martini F., Bergami F., von Daake S., Li S., Lee D., Demeler B., Hendrickson WA., Comoletti D. Only protein-protein interactions imex curation ranaivoson-2015-4 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Ranaivoson FM., Liu Q., Martini F., Bergami F., von Daake S., Li S., Lee D., Demeler B., Hendrickson WA., Comoletti D. DIP original experiment: DIP-267239X Structure (London, England : 1993) 2015 IMEx Structural and Mechanistic Insights into the Latrophilin3-FLRT3 Complex that Mediates Glutamatergic Synapse Development. Ranaivoson FM., Liu Q., Martini F., Bergami F., von Daake S., Li S., Lee D., Demeler B., Hendrickson WA., Comoletti D. Only protein-protein interactions imex curation ranaivoson-2015-5 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ranaivoson FM., Liu Q., Martini F., Bergami F., von Daake S., Li S., Lee D., Demeler B., Hendrickson WA., Comoletti D. DIP original experiment: DIP-267236X Structure (London, England : 1993) 2015 IMEx Structural and Mechanistic Insights into the Latrophilin3-FLRT3 Complex that Mediates Glutamatergic Synapse Development. Ranaivoson FM., Liu Q., Martini F., Bergami F., von Daake S., Li S., Lee D., Demeler B., Hendrickson WA., Comoletti D. Only protein-protein interactions imex curation ranaivoson-2015-6 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Ranaivoson FM., Liu Q., Martini F., Bergami F., von Daake S., Li S., Lee D., Demeler B., Hendrickson WA., Comoletti D. DIP original experiment: DIP-267238X Structure (London, England : 1993) 2015 IMEx Structural and Mechanistic Insights into the Latrophilin3-FLRT3 Complex that Mediates Glutamatergic Synapse Development. Ranaivoson FM., Liu Q., Martini F., Bergami F., von Daake S., Li S., Lee D., Demeler B., Hendrickson WA., Comoletti D. Only protein-protein interactions imex curation ranaivoson-2015-7 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Ranaivoson FM., Liu Q., Martini F., Bergami F., von Daake S., Li S., Lee D., Demeler B., Hendrickson WA., Comoletti D. DIP original experiment: DIP-267240X Structure (London, England : 1993) 2015 IMEx Structural and Mechanistic Insights into the Latrophilin3-FLRT3 Complex that Mediates Glutamatergic Synapse Development. Ranaivoson FM., Liu Q., Martini F., Bergami F., von Daake S., Li S., Lee D., Demeler B., Hendrickson WA., Comoletti D. Only protein-protein interactions imex curation flrt3_mouse Leucine-rich repeat transmembrane protein FLRT3 Flrt3 Kiaa1469 Fibronectin leucine rich transmembrane protein 3 protein protein mouse Mus musculus Mouse MISPAWSLFLIGTKIGLFFQVAPLSVVAKSCPSVCRCDAGFIYCNDRSLTSIPVGIPEDATTLYLQNNQINNVGIPSDLKNLLKVQRIYLYHNSLDEFPTNLPKYVKELHLQENNIRTITYDSLSKIPYLEELHLDDNSVSAVSIEEGAFRDSNYLRLLFLSRNHLSTIPGGLPRTIEELRLDDNRISTISSPSLHGLTSLKRLVLDGNLLNNHGLGDKVFFNLVNLTELSLVRNSLTAAPVNLPGTSLRKLYLQDNHINRVPPNAFSYLRQLYRLDMSNNNLSNLPQGIFDDLDNITQLILRNNPWYCGCKMKWVRDWLQSLPVKVNVRGLMCQAPEKVRGMAIKDLSAELFDCKDSGIVSTIQITTAIPNTAYPAQGQWPAPVTKQPDIKNPKLIKDQRTTGSPSRKTILITVKSVTPDTIHISWRLALPMTALRLSWLKLGHSPAFGSITETIVTGERSEYLVTALEPESPYRVCMVPMETSNLYLFDETPVCIETQTAPLRMYNPTTTLNREQEKEPYKNPNLPLAAIIGGAVALVSIALLALVCWYVHRNGSLFSRNCAYSKGRRRKDDYAEAGTKKDNSILEIRETSFQMLPISNEPISKEEFVIHTIFPPNGMNLYKNNLSESSSNRSYRDSGIPDSDHSHS agrl3_mouse Adhesion G protein-coupled receptor L3 Adgrl3 Kiaa0768 Latrophilin-3 Lec3 Lectomedin-3 Lphn3 protein protein mouse Mus musculus Mouse MWPPQLLILTMLLAPVVHGGKHNERHPALAAPLRHAERSPGGALPPRHLLQQPAAERSTAHRGQGPRGAARGVRGPGAPGAQIAAQAFSRAPIPMAVVRRELSCESYPIELRCPGTDVIMIESANYGRTDDKICDSDPAQMENIRCYLPDAYKIMSQRCNNRTQCAVVAGPDVFPDPCPGTYKYLEVQYECVPYKVEQKVFLCPGLLKGVYQSEHLFESDHQSGAWCKDPLQASDKIYYMPWTPYRTDTLTEYSSKDDFIAGRPTTTYKLPHRVDGTGFVVYDGALFFNKERTRNIVKFDLRTRIKSGEAIIANANYHDTSPYRWGGKSDIDLAVDENGLWVIYATEQNNGKIVISQLNPYTLRIEGTWDTAYDKRSASNAFMICGILYVVKSVYEDDDNEATGNKIDYIYNTDQSKDSLVDVPFPNSYQYIAAVDYNPRDNLLYVWNNYHVVKYSLDFGPLDSRSGPVHHGQVSYISPPIHLDSELERPPVRGISTTGSLGMGSTTTSTTLRTTTWNIGRSTTASLPGRRNRSTSTPSPAVEVLDDVTTHLPSAASQIPAMEESCEAVEAREIMWFKTRQGQVAKQPCPAGTIGVSTYLCLAPDGIWDPQGPDLSNCSSPWVNHITQKLKSGETAANIARELAEQTRNHLNAGDITYSVRAMDQLVGLLDVQLRNLTPGGKDSAARSLNKLQKRERSCRAYVQAMVETVNNLLQPQALNAWRDLTTSDQLRAATMLLDTVEESAFVLADNLLKTDIVRENTDNIQLEVARLSTEGNLEDLKFPENMGHGSTIQLSANTLKQNGRNGEIRVAFVLYNNLGPYLSTENASMKLGTEAMSTNHSVIVNSPVITAAINKEFSNKVYLADPVVFTVKHIKQSEENFNPNCSFWSYSKRTMTGYWSTQGCRLLTTNKTHTTCSCNHLTNFAVLMAHVEVKHSDAVHDLLLDVITWVGILLSLVCLLICIFTFCFFRGLQSDRNTIHKNLCISLFVAELLFLIGINRTDQPIACAVFAALLHFFFLAAFTWMFLEGVQLYIMLVEVFESEHSRRKYFYLVGYGMPALIVAVSAAVDYRSYGTDKVCWLRLDTYFIWSFIGPATLIIMLNVIFLGIALYKMFHHTAILKPESGCLDNINYEDNRPFIKSWVIGAIALLCLLGLTWAFGLMYINESTVIMAYLFTIFNSLQGMFIFIFHCVLQKKVRKEYGKCLRTHCCSGKSTESSIGSGKTSGSRTPGRYSTGSQSRIRRMWNDTVRKQSESSFITGDINSSASLNREGLLNNARDTSVMDTLPLNGNHGNSYSIAGGEYLSNCVQIIDRGYNHNETALEKKILKELTSNYIPSYLNNHERSSEQNRNMMNKLVNNLGSGSEDDAIVLDDAASFNHEESLGLELIHEESDAPLLPPRVYSTDNHQPHHYSRRRFPQDHSESFFPLLTDEHTEDLQSPHRDSLYTSMPALAGVPAADSVTTSTQTEAAAAKGGDAEDVYYKSMPNLGSRNHVHPLHAYYQLGRGSSDGFIVPPNKDGASPEGTSKGPAHLVTSL adgrl3-flrt3-4 3281379 N/A 3281386 unspecified role unspecified role unspecified role unspecified role Region 29-386 Region 29-386 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3281387 unspecified role unspecified role unspecified role unspecified role Region 199-495 Region 199-495 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false adgrl3-flrt3-5 3281380 N/A 3281386 unspecified role unspecified role unspecified role unspecified role Region 29-386 Region 29-386 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3281387 unspecified role unspecified role unspecified role unspecified role Region 199-495 Region 199-495 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q80TS3:p.Asp332Ala D332A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q80TS3:p.Tyr323Ala Y323A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false adgrl3-flrt3-6 3281381 N/A 3281386 unspecified role unspecified role unspecified role unspecified role Region 29-386 Region 29-386 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3281387 unspecified role unspecified role unspecified role unspecified role Region 199-495 Region 199-495 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false adgrl3-flrt3-7 3281382 N/A 3281386 unspecified role unspecified role unspecified role unspecified role Region 29-386 Region 29-386 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3281387 unspecified role unspecified role unspecified role unspecified role Region 199-495 Region 199-495 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false adgrl3-flrt3-8 3281383 N/A 3281386 unspecified role unspecified role unspecified role unspecified role Region 29-386 Region 29-386 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3281387 unspecified role unspecified role unspecified role unspecified role Region 199-495 Region 199-495 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false adgrl3-flrt3-9 3281384 N/A 3281386 unspecified role unspecified role unspecified role unspecified role Region 29-386 Region 29-386 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q8BGT1:p.Arg117Thr R117T mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q8BGT1:p.Phe160Ala F160A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q8BGT1:p.Phe160Asn F160N mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3281387 unspecified role unspecified role unspecified role unspecified role Region 199-495 Region 199-495 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false flrt3-2 3281385 N/A 3281386 unspecified role unspecified role unspecified role unspecified role Region 29-386 Region 29-386 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human direct interaction direct interaction false false false