DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ gaudet-2015-1 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. DIP original experiment: DIP-266257X Science (New York, N.Y.) 2015 IMEx INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. Only protein-protein interactions imex curation gaudet-2015-2 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. DIP original experiment: DIP-266259X Science (New York, N.Y.) 2015 IMEx INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. Only protein-protein interactions imex curation gaudet-2015-3 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. DIP original experiment: DIP-266261X Science (New York, N.Y.) 2015 IMEx INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. Only protein-protein interactions imex curation gaudet-2015-4 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. DIP original experiment: DIP-266258X Science (New York, N.Y.) 2015 IMEx INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. Only protein-protein interactions imex curation gaudet-2015-5 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. DIP original experiment: DIP-266262X Science (New York, N.Y.) 2015 IMEx INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. Only protein-protein interactions imex curation gaudet-2015-6 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. DIP original experiment: DIP-266260X Science (New York, N.Y.) 2015 IMEx INNATE IMMUNITY. Cytosolic detection of the bacterial metabolite HBP activates TIFA-dependent innate immunity. Gaudet RG., Sintsova A., Buckwalter CM., Leung N., Cochrane A., Li J., Cox AD., Moffat J., Gray-Owen SD. Only protein-protein interactions imex curation traf6_human TNF receptor-associated factor 6 TRAF6 RNF85 Interleukin-1 signal transducer RING finger protein 85 E3 ubiquitin-protein ligase TRAF6 RING-type E3 ubiquitin transferase TRAF6 protein protein human Homo sapiens Human MSLLNCENSCGSSQSESDCCVAMASSCSAVTKDDSVGGTASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCPQCQRPFQKFHINIHILKDCPRRQVSCDNCAASMAFEDKEIHDQNCPLANVICEYCNTILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQSHMRMLAQAVHSLSVIPDSGYISEVRNFQETIHQLEGRLVRQDHQIRELTAKMETQSMYVSELKRTIRTLEDKVAEIEAQQCNGIYIWKIGNFGMHLKCQEEEKPVVIHSPGFYTGKPGYKLCMRLHLQLPTAQRCANYISLFVHTMQGEYDSHLPWPFQGTIRLTILDQSEAPVRQNHEEIMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQRTFIKDDTLLVRCEVSTRFDMGSLRREGFQPRSTDAGV tifa_human TRAF-interacting protein with FHA domain-containing protein A Putative NF-kappa-B-activating protein 20 TIFA T2BP TRAF2-binding protein Putative MAPK-activating protein PM14 protein protein human Homo sapiens Human MTSFEDADTEETVTCLQMTVYHPGQLQCGIFQSISFNREKLPSSEVVKFGRNSNICHYTFQDKQVSRVQFSLQLFKKFNSSVLSFEIKNMSKKTNLIVDSRELGYLNKMDLPYRCMVRFGEYQFLMEKEDGESLEFFETQFILSPRSLLQENNWPPHRPIPEYGTYSLCSSQSSSPTEMDENES ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG tifa-traf6-2 3279837 N/A 3279843 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3279844 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false tifa-traf6-4 3279838 N/A 3279845 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3279843 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3279844 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human association association false false false traf6-ub-3 3279839 N/A 3279845 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3279843 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false tifa-traf6-5 3279840 N/A 3279843 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3279844 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q96CG3:p.Thr9Ala T9A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q96CG3:p.[Gly50Glu;Ser66Ala] G50E/S66A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q96CG3:p.Glu178Ala E178A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false tifa-ub-1 3279841 N/A 3279845 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3279844 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false traf6-tifa-2 3279842 N/A 3279845 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3279843 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3279844 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human association association false false false