DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ suzuki-2015-1 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Suzuki H., Kaizuka T., Mizushima N., Noda NN. DIP original experiment: DIP-266068X Nature structural & molecular biology 2015 IMEx Structure of the Atg101-Atg13 complex reveals essential roles of Atg101 in autophagy initiation. Suzuki H., Kaizuka T., Mizushima N., Noda NN. Only protein-protein interactions imex curation suzuki-2015-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Suzuki H., Kaizuka T., Mizushima N., Noda NN. DIP original experiment: DIP-266066X Nature structural & molecular biology 2015 IMEx Structure of the Atg101-Atg13 complex reveals essential roles of Atg101 in autophagy initiation. Suzuki H., Kaizuka T., Mizushima N., Noda NN. Only protein-protein interactions imex curation suzuki-2015-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Suzuki H., Kaizuka T., Mizushima N., Noda NN. DIP original experiment: DIP-266070X Nature structural & molecular biology 2015 IMEx Structure of the Atg101-Atg13 complex reveals essential roles of Atg101 in autophagy initiation. Suzuki H., Kaizuka T., Mizushima N., Noda NN. Only protein-protein interactions imex curation suzuki-2015-4 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Suzuki H., Kaizuka T., Mizushima N., Noda NN. DIP original experiment: DIP-266069X Nature structural & molecular biology 2015 IMEx Structure of the Atg101-Atg13 complex reveals essential roles of Atg101 in autophagy initiation. Suzuki H., Kaizuka T., Mizushima N., Noda NN. Only protein-protein interactions imex curation suzuki-2015-5 mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Suzuki H., Kaizuka T., Mizushima N., Noda NN. DIP original experiment: DIP-266072X Nature structural & molecular biology 2015 IMEx Structure of the Atg101-Atg13 complex reveals essential roles of Atg101 in autophagy initiation. Suzuki H., Kaizuka T., Mizushima N., Noda NN. Only protein-protein interactions imex curation suzuki-2015-6 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Suzuki H., Kaizuka T., Mizushima N., Noda NN. DIP original experiment: DIP-266071X Nature structural & molecular biology 2015 IMEx Structure of the Atg101-Atg13 complex reveals essential roles of Atg101 in autophagy initiation. Suzuki H., Kaizuka T., Mizushima N., Noda NN. Only protein-protein interactions imex curation suzuki-2015-7 in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Suzuki H., Kaizuka T., Mizushima N., Noda NN. DIP original experiment: DIP-266067X Nature structural & molecular biology 2015 IMEx Structure of the Atg101-Atg13 complex reveals essential roles of Atg101 in autophagy initiation. Suzuki H., Kaizuka T., Mizushima N., Noda NN. Only protein-protein interactions imex curation suzuki-2015-8 mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Suzuki H., Kaizuka T., Mizushima N., Noda NN. DIP original experiment: DIP-266073X Nature structural & molecular biology 2015 IMEx Structure of the Atg101-Atg13 complex reveals essential roles of Atg101 in autophagy initiation. Suzuki H., Kaizuka T., Mizushima N., Noda NN. Only protein-protein interactions imex curation mug66_schpo Meiotically up-regulated gene 66 protein mug66 Autophagy-related protein 101 atg101 SPAC25H1.03 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MTNTVTIELKIGYKYAAEVVKAVLGVILFHRQFSTVPARTIDVLDITVPTLVGAELNEQLATKAAEFIDTIRNEAGANGQMILLLYERSPKKSWFGKGNTIPWEQWILHTTILEEGDSYQESSLSLEAAVEQIVQAVNLRSLSYLPPVAMDSGNYPYEIVTPTSTEGWGSLLKRMIIENVSGGD 9F08647CBF182A6B atg13_schpo Autophagy protein 13 atg13 Meiotically up-regulated gene 78 protein mug78 SPAC4F10.07c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MPRLNTQLPRMYSAPPGHSKAVSTELNKDLSSVGGRSAKLGQVIHHCFYKTGLIILESRLNVFGTSRPRESSKNNKWFNLEIVETELYAEQFKIWKNIELSPSRKIPPMVLHTYLDISDLSKNQTLSVSDGTHSHAINFNNMSTMKIVLERWIVNLDGEALSTPLELAVLYKKLVVLFRSLYTYTHLMPLWKLKSKIHKLRAHGTSLKVGCALSTDDVLSNDFLPISAPISSSLGSSIATFSFSPVGTPAGDFRISVQYRKNCHFEVHDSDALLSNQLLSADKHQLAASNNSQDFEDGKQYDQPPPSFATRLAKQSDPNSLLQSEIQHLASIESITAQAAPLVTIHPFKSPSLSASPGSNFDNMSISPKVAVNRYIHRGPSATSLNKFSMISDAASKSRAKLPPLTSGSLKLNTLDISNTPNLRRFSSSFGPRERKESFSSRNRLPLVNHPIRSIFKHNVSENPITDHSEHAVYDSEFASKDDLSGFIQLLDSHAHHLNASEGSKSSGSFPGKVQTLTSGISPVAHPHNSLGSSNEIFDIDTYNHSIDNSGSRFTEAVKHNLGNSSHSIMRHHTLGTLRSRPSFSEKSTFPAPLTSISQASTFQGDNRSPSTVIPHTQTEVPSANDTSKQLASLHDMRKSQSPICARSATSAGLPRFEYHTSLSKSLEHSSTPASLQATKTPSPSFVLEPGIPQEYKKHFDNLSEERRQCLTPSTPTYEYYNEHNPNYDDDLLFTMTDMTLEPHDVSAIRLGSPKSDD A6C9E32818607C84 atg13_mouse Autophagy-related protein 13 Atg13 D2Ertd391e Kiaa0652 protein protein mouse Mus musculus Mouse METELSSQDRKDLDKFIKFFALKTVQVIVQARLGEKICTRSSSSPTGSDWFNLAIKDIPEVTHEAKKALSGQLPAVGRSMCVEISLKTSEGDSMELEIWCLEMNEKCDKEIKVSYTVYNRLSLLLKSLLAITRVTPAYRLSRKQGHEYVILYRIYFGEVQLNGLGEGFQTVRVGTVGTPVGTLTLSCAYRINLAFMSTRQFERTPPIMGIIIDHFVDRPYPSSSPMHPCNYRTAEDAGVAYPSVEDSQEVCTTSFSTSPPSQLSSSRLSYQPAVLGLGSADLAYPVVFTAGLNTTHAHQLMVPGKEGGVTLAPSHPTHGAQADPERLVMHMPSDGTHCAATPSSSEDTETVSNSSEGRASPHDILETIFVRKVGAFVNKPINQVTVTSLDIPFAMFAPKNLELEDADPMVNPPESPETTSPLHGSLHSDGSSGGSGGSTHDDFVMIDFKPAFSKDDILPMDLGTFYREFQNPPQLSSLSIDFGAQSMAEDLDSLPEKLAVHEKNVREFDAFVETLQ mint homomint atga1_human Autophagy-related protein 101 ATG101 C12orf44 PP894 protein protein human Homo sapiens Human MNCRSEVLEVSVEGRQVEEAMLAVLHTVLLHRSTGKFHYKKEGTYSIGTVGTQDVDCDFIDFTYVRVSSEELDRALRKVVGEFKDALRNSGGDGLGQMSLEFYQKKKSRWPFSDECIPWEVWTVKVHVVALATEQERQICREKVGEKLCEKIINIVEVMNRHEYLPKMPTQSEVDNVFDTGLRDVQPYLYKISFQITDALGTSVTTTMRRLIKDTLAL spatg101-spatg13-1 3279223 N/A 3279231 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3279232 unspecified role unspecified role unspecified role unspecified role Region 32-269 Region 32-269 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false spatg101-spatg13-2 3279224 N/A 3279231 unspecified role unspecified role unspecified role unspecified role O13978:p.[Phe29Arg;His30Arg] F29R/H30R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3279232 unspecified role unspecified role unspecified role unspecified role Region 32-269 Region 32-269 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false spatg101-spatg13-3 3279225 N/A 3279231 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3279232 unspecified role unspecified role unspecified role unspecified role Region 32-269 Region 32-269 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O36019:p.[Val176Ala;Arg179Ala] V176A/R179A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O36019:p.[Thr183Arg;Leu187Arg] T183R/L187R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false spatg101-spatg13-4 3279226 N/A 3279231 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false O13978:p.Phe29Arg F29R mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false O13978:p.[Phe29Arg;His30Arg] F29R/H30R mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3279232 unspecified role unspecified role prey prey Region 32-269 Region 32-269 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false atg101-atg13-5 3279227 N/A 3279233 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3279234 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9BSB4:p.Thr63Arg T63R mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9BSB4:p.[Leu30Arg;His31Arg] L30R/H31R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false mouse Mus musculus Mouse physical association physical association false false false spatg101-spatg13-5 3279228 N/A 3279231 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3279232 unspecified role unspecified role prey prey Region 32-269 Region 32-269 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O36019:p.[Val176Ala;Arg179Ala] V176A/R179A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O36019:p.[Thr183Arg;Leu187Arg] T183R/L187R mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false spatg101-spatg13-6 3279229 N/A 3279231 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3279232 unspecified role unspecified role unspecified role unspecified role Region 32-269 Region 32-269 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false atg101-atg13-6 3279230 N/A 3279233 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3279234 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q9BSB4:p.[Leu30Arg;His31Arg] L30R/H31R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false mouse Mus musculus Mouse physical association physical association false false false