DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ xie-2015-1 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Xie T., Zmyslowski AM., Zhang Y., Radhakrishnan I. DIP original experiment: DIP-265772X Structure (London, England : 1993) 2015 IMEx Structural Basis for Multi-specificity of MRG Domains. Xie T., Zmyslowski AM., Zhang Y., Radhakrishnan I. Only protein-protein interactions imex curation xie-2015-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Xie T., Zmyslowski AM., Zhang Y., Radhakrishnan I. DIP original experiment: DIP-265770X Structure (London, England : 1993) 2015 IMEx Structural Basis for Multi-specificity of MRG Domains. Xie T., Zmyslowski AM., Zhang Y., Radhakrishnan I. Only protein-protein interactions imex curation xie-2015-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Xie T., Zmyslowski AM., Zhang Y., Radhakrishnan I. DIP original experiment: DIP-265775X Structure (London, England : 1993) 2015 IMEx Structural Basis for Multi-specificity of MRG Domains. Xie T., Zmyslowski AM., Zhang Y., Radhakrishnan I. Only protein-protein interactions imex curation xie-2015-4 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Xie T., Zmyslowski AM., Zhang Y., Radhakrishnan I. DIP original experiment: DIP-265771X Structure (London, England : 1993) 2015 IMEx Structural Basis for Multi-specificity of MRG Domains. Xie T., Zmyslowski AM., Zhang Y., Radhakrishnan I. Only protein-protein interactions imex curation xie-2015-5 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Xie T., Zmyslowski AM., Zhang Y., Radhakrishnan I. DIP original experiment: DIP-265774X Structure (London, England : 1993) 2015 IMEx Structural Basis for Multi-specificity of MRG Domains. Xie T., Zmyslowski AM., Zhang Y., Radhakrishnan I. Only protein-protein interactions imex curation xie-2015-6 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Xie T., Zmyslowski AM., Zhang Y., Radhakrishnan I. DIP original experiment: DIP-265773X Structure (London, England : 1993) 2015 IMEx Structural Basis for Multi-specificity of MRG Domains. Xie T., Zmyslowski AM., Zhang Y., Radhakrishnan I. Only protein-protein interactions imex curation mrgbp_human MRG/MORF4L-binding protein MRGBP C20orf20 MRG-binding protein Up-regulated in colon cancer 4 protein protein human Homo sapiens Human MGEAEVGGGGAAGDKGPGEAATSPAEETVVWSPEVEVCLFHAMLGHKPVGVNRHFHMICIRDKFSQNIGRQVPSKVIWDHLSTMYDMQALHESEILPFPNPERNFVLPEEIIQEVREGKVMIEEEMKEEMKEDVDPHNGADDVFSSSGSLGKASEKSSKDKEKNSSDLGCKEGADKRKRSRVTDKVLTANSNPSSPSAAKRRRT q9ubu8-2 Mortality factor 4-like protein 1 MORF4L1 MORF-related gene 15 protein MRG15 Protein MSL3-1 Transcription factor-like protein MRG15 FWP006 HSPC008 HSPC061 PP368 protein protein human Homo sapiens Human MAPKQDPKPKFQEGERVLCFHGPLLYEAKCVKVAIKDKQVKYFIHYSGWNKNWDEWVPESRVLKYVDTNLQKQRELQKANQEQYAEGKMRGAAPGKKTSGLQQKNVEVKTKKNKQKTPGNGDGGSTSETPQPPRKKRARVDPTVENEETFMNRVEVKVKIPEELKPWLVDDWDLITRQKQLFYLPAKKNVDSILEDYANYKKSRGNTDNKEYAVNEVVAGIKEYFNVMLGTQLLYKFERPQYAEILADHPDAPMSQVYGAPHLLRLFVRIGAMLAYTPLDEKSLALLLNYLHDFLKYLAKNSATLFSASDYEVAPPEYHRKAV palb2_human Partner and localizer of BRCA2 PALB2 FANCN protein protein human Homo sapiens Human MDEPPGKPLSCEEKEKLKEKLAFLKREYSKTLARLQRAQRAEKIKHSIKKTVEEQDCLSQQDLSPQLKHSEPKNKICVYDKLHIKTHLDEETGEKTSITLDVGPESFNPGDGPGGLPIQRTDDTQEHFPHRVSDPSGEQKQKLPSRRKKQQKRTFISQERDCVFGTDSLRLSGKRLKEQEEISSKNPARSPVTEIRTHLLSLKSELPDSPEPVTEINEDSVLIPPTAQPEKGVDTFLRRPNFTRATTVPLQTLSDSGSSQHLEHIPPKGSSELTTHDLKNIRFTSPVSLEAQGKKMTVSTDNLLVNKAISKSGQLPTSSNLEANISCSLNELTYNNLPANENQNLKEQNQTEKSLKSPSDTLDGRNENLQESEILSQPKSLSLEATSPLSAEKHSCTVPEGLLFPAEYYVRTTRSMSNCQRKVAVEAVIQSHLDVKKKGFKNKNKDASKNLNLSNEETDQSEIRMSGTCTGQPSSRTSQKLLSLTKVSSPAGPTEDNDLSRKAVAQAPGRRYTGKRKSACTPASDHCEPLLPTSSLSIVNRSKEEVTSHKYQHEKLFIQVKGKKSRHQKEDSLSWSNSAYLSLDDDAFTAPFHRDGMLSLKQLLSFLSITDFQLPDEDFGPLKLEKVKSCSEKPVEPFESKMFGERHLKEGSCIFPEELSPKRMDTEMEDLEEDLIVLPGKSHPKRPNSQSQHTKTGLSSSILLYTPLNTVAPDDNDRPTTDMCSPAFPILGTTPAFGPQGSYEKASTEVAGRTCCTPQLAHLKDSVCLASDTKQFDSSGSPAKPHTTLQVSGRQGQPTCDCDSVPPGTPPPIESFTFKENQLCRNTCQELHKHSVEQTETAELPASDSINPGNLQLVSELKNPSGSCSVDVSAMFWERAGCKEPCIITACEDVVSLWKALDAWQWEKLYTWHFAEVPVLQIVPVPDVYNLVCVALGNLEIREIRALFCSSDDESEKQVLLKSGNIKAVLGLTKRRLVSSSGTLSDQQVEVMTFAEDGGGKENQFLMPPEETILTFAEVQGMQEALLGTTIMNNIVIWNLKTGQLLKKMHIDDSYQASVCHKAYSEMGLLFIVLSHPCAKESESLRSPVFQLIVINPKTTLSVGVMLYCLPPGQAGRFLEGDVKDHCAAAILTSGTIAIWDLLLGQCTALLPPVSDQHWSFVKWSGTDSHLLAGQKDGNIFVYHYS morf4l1-mrgbp-9 3278724 N/A 3278730 unspecified role unspecified role unspecified role unspecified role Region 69-119 Region 69-119 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9NV56:p.Trp78Ala W78A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9NV56:p.Phe105Ala F105A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9NV56:p.Met84Ala M84A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9NV56:p.Ile112Ala I112A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3278731 unspecified role unspecified role unspecified role unspecified role Region 155-323 Region 155-323 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false morf4l1-mrgbp-10 3278725 N/A 3278730 unspecified role unspecified role unspecified role unspecified role Region 69-119 Region 69-119 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3278731 unspecified role unspecified role unspecified role unspecified role Region 155-323 Region 155-323 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false morf4l1-mrgbp-11 3278726 N/A 3278730 unspecified role unspecified role unspecified role unspecified role Region 69-119 Region 69-119 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3278731 unspecified role unspecified role unspecified role unspecified role Region 155-323 Region 155-323 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false morf4l1-mrgbp-12 3278727 N/A 3278730 unspecified role unspecified role unspecified role unspecified role Region 69-119 Region 69-119 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3278731 unspecified role unspecified role unspecified role unspecified role Region 155-323 Region 155-323 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false morf4l1-palb2-1 3278728 N/A 3278732 unspecified role unspecified role unspecified role unspecified role Region 579-629 Region 579-629 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3278731 unspecified role unspecified role unspecified role unspecified role Region 155-323 Region 155-323 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false morf4l1-mrgbp-13 3278729 N/A 3278730 unspecified role unspecified role unspecified role unspecified role Region 69-119 Region 69-119 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3278731 unspecified role unspecified role unspecified role unspecified role Region 155-323 Region 155-323 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9UBU8-2:p.Leu168Ala L168A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9UBU8-2:p.Trp172Ala W172A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9UBU8-2:p.Leu287Ala L287A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false