DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ li-2015-27 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. DIP original experiment: DIP-266210X Nature chemical biology 2015 IMEx Impeding the interaction between Nur77 and p38 reduces LPS-induced inflammation. Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. Only protein-protein interactions imex curation li-2015-28 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. DIP original experiment: DIP-266220X Nature chemical biology 2015 IMEx Impeding the interaction between Nur77 and p38 reduces LPS-induced inflammation. Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. Only protein-protein interactions imex curation li-2015-29 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. DIP original experiment: DIP-266216X Nature chemical biology 2015 IMEx Impeding the interaction between Nur77 and p38 reduces LPS-induced inflammation. Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. Only protein-protein interactions imex curation li-2015-30 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. DIP original experiment: DIP-266213X Nature chemical biology 2015 IMEx Impeding the interaction between Nur77 and p38 reduces LPS-induced inflammation. Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. Only protein-protein interactions imex curation li-2015-31 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. DIP original experiment: DIP-266218X Nature chemical biology 2015 IMEx Impeding the interaction between Nur77 and p38 reduces LPS-induced inflammation. Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. Only protein-protein interactions imex curation li-2015-32 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. DIP original experiment: DIP-266211X Nature chemical biology 2015 IMEx Impeding the interaction between Nur77 and p38 reduces LPS-induced inflammation. Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. Only protein-protein interactions imex curation li-2015-33 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. DIP original experiment: DIP-266217X Nature chemical biology 2015 IMEx Impeding the interaction between Nur77 and p38 reduces LPS-induced inflammation. Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. Only protein-protein interactions imex curation li-2015-34 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. DIP original experiment: DIP-266215X Nature chemical biology 2015 IMEx Impeding the interaction between Nur77 and p38 reduces LPS-induced inflammation. Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. Only protein-protein interactions imex curation li-2015-35 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. DIP original experiment: DIP-266212X Nature chemical biology 2015 IMEx Impeding the interaction between Nur77 and p38 reduces LPS-induced inflammation. Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. Only protein-protein interactions imex curation li-2015-36 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. DIP original experiment: DIP-266214X Nature chemical biology 2015 IMEx Impeding the interaction between Nur77 and p38 reduces LPS-induced inflammation. Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. Only protein-protein interactions imex curation li-2015-37 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. DIP original experiment: DIP-266219X Nature chemical biology 2015 IMEx Impeding the interaction between Nur77 and p38 reduces LPS-induced inflammation. Li L., Liu Y., Chen HZ., Li FW., Wu JF., Zhang HK., He JP., Xing YZ., Chen Y., Wang WJ., Tian XY., Li AZ., Zhang Q., Huang PQ., Han J., Lin T., Wu Q. Only protein-protein interactions imex curation tf65_mouse Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit Rela Nfkb3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein mouse Mus musculus Mouse MDDLFPLIFPSEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGYYEADLCPDRSIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFHVPIEEQRGDYDLNAVRLCFQVTVRDPAGRPLLLTPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFNGPTEPRPPTRRIAVPTRNSTSVPKPAPQPYTFPASLSTINFDEFSPMLLPSGQISNQALALAPSSAPVLAQTMVPSSAMVPLAQPPAPAPVLTPGPPQSLSAPVPKSTQAGEGTLSEALLHLQFDADEDLGALLGNSTDPGVFTDLASVDNSEFQQLLNQGVSMSHSTAEPMLMEYPEAITRLVTGSQRPPDPAPTPLGTSGLPNGLSGDEDFSSIADMDFSALLSQISS mk14_mouse Mitogen-activated protein kinase 14 Mapk14 Crk1 Csbp1 Csbp2 Mitogen-activated protein kinase p38 alpha CRK1 protein protein mouse Mus musculus Mouse MSQERPTFYRQELNKTIWEVPERYQNLSPVGSGAYGSVCAAFDTKTGHRVAVKKLSRPFQSIIHAKRTYRELRLLKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMGADLNNIVKCQKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARHTDDEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLAQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQYHDPDDEPVADPYDQSFESRDLLIDEWKSLTYDEVISFVPPPLDQEEMES nr4a1_mouse Nuclear receptor subfamily 4 group A member 1 Nr4a1 Gfrp Hmr N10 Nur77 Nuclear hormone receptor NUR/77 Nuclear protein N10 Orphan nuclear receptor HMR protein protein mouse Mus musculus Mouse MPCIQAQYGTPATSPGPRDHLTGDPLALEFGKPTMDLASPETAPAAPATLPSFSTFMDGYTGEFDTFLYQLPGTTQPCSSACSSASSTSSSSSSATSPASASFKFEDFQVYGCYPGTLSGPLDETLSSSGSEYYGSPCSAPSPSTPNFQPSQLSPWDGSFGHFSPSQTYEGLWAWTEQLPKASSGPPPPPTFFSFSPPTGPSPSLAQSSLKLFPPPATHQLGEGESYSMPAAFPGLAPTSPNRDTSGILDAPVTSTKSRSGASGGSEGRCAVCGDNASCQHYGVRTCEGCKGFFKRTVQKSAKYICLANKDCPVDKRRRNRCQFCRFQKCLAVGMVKEVVRTDSLKGRRGRLPSKPKQPPDASPTNLLTSLIRAHLDSGPSTAKLDYSKFQELVLPRFGKEDAGDVQQFYDLLSGSLDVIRKWAEKIPGFIELCPGDQDLLLESAFLELFILRLAYRSKPGEGKLIFCSGLVLHQLQCARGFGDWIDNILAFSRSLHSLGVDVPAFACLSALVLITDRHGLQDPRRVEELQNRIASCLKEHMATVAGDPQPASCLSRLLGKLPELRTLCTQGLQRIFCLKLEDLVPPPPIVDKIFMDTLSF stk11_human Serine/threonine-protein kinase STK11 STK11 LKB1 PJS Liver kinase B1 Renal carcinoma antigen NY-REN-19 protein protein human Homo sapiens Human MEVVDPQQLGMFTEGELMSVGMDTFIHRIDSTEVIYQPRRKRAKLIGKYLMGDLLGEGSYGKVKEVLDSETLCRRAVKILKKKKLRRIPNGEANVKKEIQLLRRLRHKNVIQLVDVLYNEEKQKMYMVMEYCVCGMQEMLDSVPEKRFPVCQAHGYFCQLIDGLEYLHSQGIVHKDIKPGNLLLTTGGTLKISDLGVAEALHPFAADDTCRTSQGSPAFQPPEIANGLDTFSGFKVDIWSAGVTLYNITTGLYPFEGDNIYKLFENIGKGSYAIPGDCGPPLSDLLKGMLEYEPAKRFSIRQIRQHSWFRKKHPPAEAPVPIPPSPDTKDRWRSMTVVPYLEDLHGADEDEDLFDIEDDIIYTQDFTVPGQVPEEEASHNGQRRGLPKAVCMNGTEAAQLSTKSRAEGRAPNPARKACSASSKIRRLSACKQQ p22736-1 Nuclear receptor subfamily 4 group A member 1 NR4A1 GFRP1 HMR NAK1 Early response protein NAK1 Nuclear hormone receptor NUR/77 Orphan nuclear receptor HMR Orphan nuclear receptor TR3 ST-59 Testicular receptor 3 protein protein human Homo sapiens Human MPCIQAQYGTPAPSPGPRDHLASDPLTPEFIKPTMDLASPEAAPAAPTALPSFSTFMDGYTGEFDTFLYQLPGTVQPCSSASSSASSTSSSSATSPASASFKFEDFQVYGCYPGPLSGPVDEALSSSGSDYYGSPCSAPSPSTPSFQPPQLSPWDGSFGHFSPSQTYEGLRAWTEQLPKASGPPQPPAFFSFSPPTGPSPSLAQSPLKLFPSQATHQLGEGESYSMPTAFPGLAPTSPHLEGSGILDTPVTSTKARSGAPGGSEGRCAVCGDNASCQHYGVRTCEGCKGFFKRTVQKNAKYICLANKDCPVDKRRRNRCQFCRFQKCLAVGMVKEVVRTDSLKGRRGRLPSKPKQPPDASPANLLTSLVRAHLDSGPSTAKLDYSKFQELVLPHFGKEDAGDVQQFYDLLSGSLEVIRKWAEKIPGFAELSPADQDLLLESAFLELFILRLAYRSKPGEGKLIFCSGLVLHRLQCARGFGDWIDSILAFSRSLHSLLVDVPAFACLSALVLITDRHGLQEPRRVEELQNRIASCLKEHVAAVAGEPQPASCLSRLLGKLPELRTLCTQGLQRIFYLKLEDLVPPPPIIDKIFMDTLPF tf65_human Transcription factor p65 Nuclear factor NF-kappa-B p65 subunit RELA NFKB3 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 protein protein human Homo sapiens Human MDELFPLIFPAEPAQASGPYVEIIEQPKQRGMRFRYKCEGRSAGSIPGERSTDTTKTHPTIKINGYTGPGTVRISLVTKDPPHRPHPHELVGKDCRDGFYEAELCPDRCIHSFQNLGIQCVKKRDLEQAISQRIQTNNNPFQVPIEEQRGDYDLNAVRLCFQVTVRDPSGRPLRLPPVLSHPIFDNRAPNTAELKICRVNRNSGSCLGGDEIFLLCDKVQKEDIEVYFTGPGWEARGSFSQADVHRQVAIVFRTPPYADPSLQAPVRVSMQLRRPSDRELSEPMEFQYLPDTDDRHRIEEKRKRTYETFKSIMKKSPFSGPTDPRPPPRRIAVPSRSSASVPKPAPQPYPFTSSLSTINYDEFPTMVFPSGQISQASALAPAPPQVLPQAPAPAPAPAMVSALAQAPAPVPVLAPGPPQAVAPPAPKPTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS q16539-1 Mitogen-activated protein kinase 14 MAPK14 CSBP CSBP1 CSBP2 CSPB1 MXI2 SAPK2A Cytokine suppressive anti-inflammatory drug-binding protein MAP kinase MXI2 MAX-interacting protein 2 Mitogen-activated protein kinase p38 alpha Stress-activated protein kinase 2a protein protein human Homo sapiens Human MSQERPTFYRQELNKTIWEVPERYQNLSPVGSGAYGSVCAAFDTKTGLRVAVKKLSRPFQSIIHAKRTYRELRLLKHMKHENVIGLLDVFTPARSLEEFNDVYLVTHLMGADLNNIVKCQKLTDDHVQFLIYQILRGLKYIHSADIIHRDLKPSNLAVNEDCELKILDFGLARHTDDEMTGYVATRWYRAPEIMLNWMHYNQTVDIWSVGCIMAELLTGRTLFPGTDHIDQLKLILRLVGTPGAELLKKISSESARNYIQSLTQMPKMNFANVFIGANPLAVDLLEKMLVLDSDKRITAAQALAHAYFAQYHDPDDEPVADPYDQSFESRDLLIDEWKSLTYDEVISFVPPPLDQEEMES nr4a1-mapk14-1 3277591 N/A 3277602 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3277603 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3277604 unspecified role unspecified role bait bait mouse Mus musculus Mouse association association false false false stk11-nr4a1-1 3277592 N/A 3277605 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3277606 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Region 377-598 Region 377-598 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false nr4a1-rela-1 3277593 N/A 3277607 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3277606 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false nr4a1-mapk14-2 3277594 N/A 3277608 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3277606 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false mapk14-nr4a1-1 3277595 N/A 3277608 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3277606 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human Homo sapiens Human physical association physical association false false false nr4a1-rela-2 3277596 N/A 3277602 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3277604 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association false false false nr4a1-rela-3 3277597 N/A 3277606 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Region 377-598 Region 377-598 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 1-265 Region 1-265 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3277607 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false rela-nr4a1-1 3277598 N/A 3277606 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human Homo sapiens Human N/A 3277607 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false mapk14-nr4a1-2 3277599 N/A 3277608 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli N/A 3277606 enzyme target enzyme target substrate substrate unspecified role unspecified role pThr pThr opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false mapk14-nr4a1-3 3277600 N/A 3277608 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3277606 unspecified role unspecified role prey prey P22736-1:p.Pro597Trp P597W mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P22736-1:p.His516Trp H516W mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Region 377-598 Region 377-598 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false mapk14-nr4a1-4 3277601 N/A 3277608 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3277606 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false Region 377-598 Region 377-598 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false