DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ parker-2015-1 in vitro In vitro thermal shift thermal shift binding thermal shift predetermined predetermined participant predetermined Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264864X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions imex curation parker-2015-2 crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster comigration in sds comigration in sds page comigration in sds anti tag western anti tag western blot anti tag western Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264871X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions imex curation parker-2015-3 ecolx Escherichia coli comigration in sds comigration in sds page comigration in sds weight by comassie molecular weight estimation by coomasie staining weight by comassie Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264867X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions imex curation parker-2015-4 in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264869X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions imex curation parker-2015-5 in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264870X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions imex curation parker-2015-6 in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264868X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions imex curation parker-2015-7 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264865X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions imex curation parker-2015-8 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. DIP original experiment: DIP-264866X Structure (London, England : 1993) 2015 IMEx Structural basis for VEGF-C binding to neuropilin-2 and sequestration by a soluble splice form. Parker MW., Linkugel AD., Goel HL., Wu T., Mercurio AM., Vander Kooi CW. Only protein-protein interactions imex curation p97953-1 Vascular endothelial growth factor C Vegfc Vascular endothelial growth factor-related protein Vegf-C Flt4 ligand protein protein mouse Mus musculus Mouse MHLLCFLSLACSLLAAALIPSPREAPATVAAFESGLGFSEAEPDGGEVKAFEGKDLEEQLRSVSSVDELMSVLYPDYWKMYKCQLRKGGWQQPTLNTRTGDSVKFAAAHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGAATNTFFKPPCVSVYRCGGCCNSEGLQCMNTSTGYLSKTLFEITVPLSQGPKPVTISFANHTSCRCMSKLDVYRQVHSIIRRSLPATLPQCQAANKTCPTNYVWNNYMCRCLAQQDFIFYSNVEDDSTNGFHDVCGPNKELDEDTCQCVCKGGLRPSSCGPHKELDRDSCQCVCKNKLFPNSCGANREFDENTCQCVCKRTCPRNQPLNPGKCACECTENTQKCFLKGKKFHHQTCSCYRRPCANRLKHCDPGLSFSEEVCRCVPSYWKRPHLN nrp2_human Neuropilin-2 NRP2 VEGF165R2 Vascular endothelial cell growth factor 165 receptor 2 protein protein human Homo sapiens Human MDMFPLTWVFLALYFSRHQVRGQPDPPCGGRLNSKDAGYITSPGYPQDYPSHQNCEWIVYAPEPNQKIVLNFNPHFEIEKHDCKYDFIEIRDGDSESADLLGKHCGNIAPPTIISSGSMLYIKFTSDYARQGAGFSLRYEIFKTGSEDCSKNFTSPNGTIESPGFPEKYPHNLDCTFTILAKPKMEIILQFLIFDLEHDPLQVGEGDCKYDWLDIWDGIPHVGPLIGKYCGTKTPSELRSSTGILSLTFHTDMAVAKDGFSARYYLVHQEPLENFQCNVPLGMESGRIANEQISASSTYSDGRWTPQQSRLHGDDNGWTPNLDSNKEYLQVDLRFLTMLTAIATQGAISRETQNGYYVKSYKLEVSTNGEDWMVYRHGKNHKVFQANNDATEVVLNKLHAPLLTRFVRIRPQTWHSGIALRLELFGCRVTDAPCSNMLGMLSGLIADSQISASSTQEYLWSPSAARLVSSRSGWFPRIPQAQPGEEWLQVDLGTPKTVKGVIIQGARGGDSITAVEARAFVRKFKVSYSLNGKDWEYIQDPRTQQPKLFEGNMHYDTPDIRRFDPIPAQYVRVYPERWSPAGIGMRLEVLGCDWTDSKPTVETLGPTVKSEETTTPYPTEEEATECGENCSFEDDKDLQLPSGFNCNFDFLEEPCGWMYDHAKWLRTTWASSSSPNDRTFPDDRNFLRLQSDSQREGQYARLISPPVHLPRSPVCMEFQYQATGGRGVALQVVREASQESKLLWVIREDQGGEWKHGRIILPSYDMEYQIVFEGVIGKGRSGEIAIDDIRISTDVPLENCMEPISAFAGENFKVDIPEIHEREGYEDEIDDEYEVDWSNSSSATSGSGAPSTDKEKSWLYTLDPILITIIAMSSLGVLLGATCAGLLLYCTCSYSGLSSRSCTTLENYNFELYDGLKHKVKMNHQKCCSEA o60462-6 Neuropilin-2 NRP2 VEGF165R2 Vascular endothelial cell growth factor 165 receptor 2 protein protein human Homo sapiens Human MDMFPLTWVFLALYFSRHQVRGQPDPPCGGRLNSKDAGYITSPGYPQDYPSHQNCEWIVYAPEPNQKIVLNFNPHFEIEKHDCKYDFIEIRDGDSESADLLGKHCGNIAPPTIISSGSMLYIKFTSDYARQGAGFSLRYEIFKTGSEDCSKNFTSPNGTIESPGFPEKYPHNLDCTFTILAKPKMEIILQFLIFDLEHDPLQVGEGDCKYDWLDIWDGIPHVGPLIGKYCGTKTPSELRSSTGILSLTFHTDMAVAKDGFSARYYLVHQEPLENFQCNVPLGMESGRIANEQISASSTYSDGRWTPQQSRLHGDDNGWTPNLDSNKEYLQVDLRFLTMLTAIATQGAISRETQNGYYVKSYKLEVSTNGEDWMVYRHGKNHKVFQANNDATEVVLNKLHAPLLTRFVRIRPQTWHSGIALRLELFGCRVTDAPCSNMLGMLSGLIADSQISASSTQEYLWSPSAARLVSSRSGWFPRIPQAQPGEEWLQVDLGTPKTVKGVIIQGARGGDSITAVEARAFVRKFKVSYSLNGKDWEYIQDPRTQQPKVGCSWRPL vgfr3_human Vascular endothelial growth factor receptor 3 Tyrosine-protein kinase receptor FLT4 FLT4 Fms-like tyrosine kinase 4 VEGFR3 protein protein human Homo sapiens Human MQRGAALCLRLWLCLGLLDGLVSGYSMTPPTLNITEESHVIDTGDSLSISCRGQHPLEWAWPGAQEAPATGDKDSEDTGVVRDCEGTDARPYCKVLLLHEVHANDTGSYVCYYKYIKARIEGTTAASSYVFVRDFEQPFINKPDTLLVNRKDAMWVPCLVSIPGLNVTLRSQSSVLWPDGQEVVWDDRRGMLVSTPLLHDALYLQCETTWGDQDFLSNPFLVHITGNELYDIQLLPRKSLELLVGEKLVLNCTVWAEFNSGVTFDWDYPGKQAERGKWVPERRSQQTHTELSSILTIHNVSQHDLGSYVCKANNGIQRFRESTEVIVHENPFISVEWLKGPILEATAGDELVKLPVKLAAYPPPEFQWYKDGKALSGRHSPHALVLKEVTEASTGTYTLALWNSAAGLRRNISLELVVNVPPQIHEKEASSPSIYSRHSRQALTCTAYGVPLPLSIQWHWRPWTPCKMFAQRSLRRRQQQDLMPQCRDWRAVTTQDAVNPIESLDTWTEFVEGKNKTVSKLVIQNANVSAMYKCVVSNKVGQDERLIYFYVTTIPDGFTIESKPSEELLEGQPVLLSCQADSYKYEHLRWYRLNLSTLHDAHGNPLLLDCKNVHLFATPLAASLEEVAPGARHATLSLSIPRVAPEHEGHYVCEVQDRRSHDKHCHKKYLSVQALEAPRLTQNLTDLLVNVSDSLEMQCLVAGAHAPSIVWYKDERLLEEKSGVDLADSNQKLSIQRVREEDAGRYLCSVCNAKGCVNSSASVAVEGSEDKGSMEIVILVGTGVIAVFFWVLLLLIFCNMRRPAHADIKTGYLSIIMDPGEVPLEEQCEYLSYDASQWEFPRERLHLGRVLGYGAFGKVVEASAFGIHKGSSCDTVAVKMLKEGATASEHRALMSELKILIHIGNHLNVVNLLGACTKPQGPLMVIVEFCKYGNLSNFLRAKRDAFSPCAEKSPEQRGRFRAMVELARLDRRRPGSSDRVLFARFSKTEGGARRASPDQEAEDLWLSPLTMEDLVCYSFQVARGMEFLASRKCIHRDLAARNILLSESDVVKICDFGLARDIYKDPDYVRKGSARLPLKWMAPESIFDKVYTTQSDVWSFGVLLWEIFSLGASPYPGVQINEEFCQRLRDGTRMRAPELATPAIRRIMLNCWSGDPKARPAFSELVEILGDLLQGRGLQEEEEVCMAPRSSQSSEEGSFSQVSTMALHIAQADAEDSPPSLQRHSLAARYYNWVSFPGCLARGAETRGSSRMKTFEEFPMTPTTYKGSVDNQTDSGMVLASEEFEQIESRHRQESGFSCKGPGQNVAVTRAHPDSQGRRRRPERGARGGQVFYNSEYGELSEPSEEDHCSPSARVTFFTDNSY nrp2-vegfc-1 3276772 N/A 3276780 unspecified role unspecified role unspecified role unspecified role Region 215-223 Region 215-223 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3276781 unspecified role unspecified role unspecified role unspecified role Region 279-595 Region 279-595 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false nrp2-2 3276773 N/A 3276782 unspecified role unspecified role unspecified role unspecified role Region 275-555 Region 275-555 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Hgh-tag Hgh-tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster disulfide bond disulfide bond disulfide bridge SS-bond false false false nrp2-3 3276774 N/A 3276782 unspecified role unspecified role unspecified role unspecified role Region 275-555 Region 275-555 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli disulfide bond disulfide bond disulfide bridge SS-bond false false false nrp2-vegfc-2 3276775 N/A 3276780 unspecified role unspecified role prey prey Region 108-223 Region 108-223 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster N/A 3276781 unspecified role unspecified role bait bait Region 279-595 Region 279-595 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O60462:p.Thr319Arg T319R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false flt4-vegfc-1 3276776 N/A 3276783 unspecified role unspecified role bait bait Region 21-776 Region 21-776 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3276780 unspecified role unspecified role prey prey Region 108-223 Region 108-223 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster direct interaction direct interaction false false false nrp2-vegfc-3 3276777 N/A 3276780 unspecified role unspecified role prey prey Region 108-223 Region 108-223 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P97953-1:p.Arg223Glu R223E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster N/A 3276781 unspecified role unspecified role bait bait Region279-595 Region279-595 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false nrp2-4 3276778 N/A 3276782 unspecified role unspecified role unspecified role unspecified role Region 275-555 Region 275-555 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false nrp2-5 3276779 N/A 3276782 unspecified role unspecified role unspecified role unspecified role Region 275-555 Region 275-555 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false