DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ seung-2015-1 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Seung D., Soyk S., Coiro M., Maier BA., Eicke S., Zeeman SC. DIP original experiment: DIP-264896X PLoS biology 2015 IMEx PROTEIN TARGETING TO STARCH is required for localising GRANULE-BOUND STARCH SYNTHASE to starch granules and for normal amylose synthesis in Arabidopsis. Seung D., Soyk S., Coiro M., Maier BA., Eicke S., Zeeman SC. Only protein-protein interactions imex curation seung-2015-2 nicbe Nicotiana benthamiana anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Seung D., Soyk S., Coiro M., Maier BA., Eicke S., Zeeman SC. DIP original experiment: DIP-264897X PLoS biology 2015 IMEx PROTEIN TARGETING TO STARCH is required for localising GRANULE-BOUND STARCH SYNTHASE to starch granules and for normal amylose synthesis in Arabidopsis. Seung D., Soyk S., Coiro M., Maier BA., Eicke S., Zeeman SC. Only protein-protein interactions imex curation ptst_arath Protein PTST, chloroplastic PTST1 MKM21.80 At5g39790 PTST PROTEIN TARGETING TO STARCH protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MGCVPRIEFGCSSQSLTLSWNLRAWNLCRLNTISHFQKLPYPLVASTRKHYKNSLLLKRFLVGVGTEESSLSEDLLDESLSRPLTSDELKSLLIDTERSKLVKKLSEANQQNRFLKRQLKTQEHEITNIKTELALMELEVQALVKLAEEIANLGIPQGSRKISGKYIQSHLLSRLDAVQKKMKEQIKGVEAAQSKEVHVFWIGMAESVQVMGSFDGWSQREDLSPEYSALFTKFSTTLFLRPGRYEMKFLVDGEWQISPEFPTSGEGLMENNVLVVE ssg1_arath Granule-bound starch synthase 1, chloroplastic/amyloplastic GBSS1 GBS1 GBSS Granule-bound starch synthase I WAXY F9L11.8 At1g32900 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MATVTASSNFVSRTSLFNNHGASSCSDVAQITLKGQSLTHCGLRSFNMVDNLQRRSQAKPVSAKSSKRSSKVKTAGKIVCEKGMSVIFIGAEVGPWSKTGGLGDVLGGLPPALAARGHRVMTICPRYDQYKDAWDTCVVVQIKVGDKVENVRFFHCYKRGVDRVFVDHPIFLAKVVGKTGSKIYGPITGVDYNDNQLRFSLLCQAALEAPQVLNLNSSKYFSGPYGEDVVFVANDWHTALLPCYLKSMYQSRGVYMNAKVVFCIHNIAYQGRFAFDDYSLLNLPISFKSSFDFMDGYEKPVKGRKINWMKAAILEAHRVLTVSPYYAQELISGVDRGVELHKYLRMKTVSGIINGMDVQEWNPSTDKYIDIKYDITTVTDAKPLIKEALQAAVGLPVDRDVPVIGFIGRLEEQKGSDILVEAISKFMGLNVQMVILGTGKKKMEAQILELEEKFPGKAVGVAKFNVPLAHMITAGADFIIVPSRFEPCGLIQLHAMRYGTVPIVASTGGLVDTVKDGYTGFHIGRFNVKCEVVDPDDVIATAKAVTRAVAVYGTSAMQEMVKNCMDQDFSWKGPARLWEKVLLSLNVAGSEAGTEGEEIAPLAKENVATP gbss1-ptst-1 3276159 N/A 3276161 unspecified role unspecified role prey prey GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 45-277 Region 45-277 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3276162 unspecified role unspecified role bait bait Q9MAQ0:p.Lys441Glu K441E mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9MAQ0:p.Glu449Lys E449K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9MAQ0:p.Glu452Lys E452K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9MAQ0:p.[Lys441Glu;Glu452Lys] K441E/E452K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region 80-610 Region 80-610 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ptst-gbss1-1 3276160 N/A 3276161 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false nicbe Nicotiana benthamiana N/A 3276162 unspecified role unspecified role prey prey Q9MAQ0:p.Glu452Lys E452K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9MAQ0:p.[Lys441Glu;Glu452Lys] K441E/E452K mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false nicbe Nicotiana benthamiana physical association physical association false false false