DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ heler-2015-1 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Heler R., Samai P., Modell JW., Weiner C., Goldberg GW., Bikard D., Marraffini LA. DIP original experiment: DIP-265465X Nature 2015 IMEx Cas9 specifies functional viral targets during CRISPR-Cas adaptation. Heler R., Samai P., Modell JW., Weiner C., Goldberg GW., Bikard D., Marraffini LA. Only protein-protein interactions imex curation heler-2015-2 ecolx Escherichia coli ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom weight by comassie molecular weight estimation by coomasie staining weight by comassie Heler R., Samai P., Modell JW., Weiner C., Goldberg GW., Bikard D., Marraffini LA. DIP original experiment: DIP-265467X Nature 2015 IMEx Cas9 specifies functional viral targets during CRISPR-Cas adaptation. Heler R., Samai P., Modell JW., Weiner C., Goldberg GW., Bikard D., Marraffini LA. Only protein-protein interactions imex curation heler-2015-3 ecolx Escherichia coli pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Heler R., Samai P., Modell JW., Weiner C., Goldberg GW., Bikard D., Marraffini LA. DIP original experiment: DIP-265466X Nature 2015 IMEx Cas9 specifies functional viral targets during CRISPR-Cas adaptation. Heler R., Samai P., Modell JW., Weiner C., Goldberg GW., Bikard D., Marraffini LA. Only protein-protein interactions imex curation q99ys8_strp1 CRISPR-associated endoribonuclease Cas2 cas2 SPy_1561 protein protein strp1 Streptococcus pyogenes serotype M1 MMVLVTYDVNTETPAGRKRLRHVAKLCVDYGQRVQNSVFECSVTPAEFVDIKHRLTQIIDEKTDSIRFYLLGKNWQRRVETLGRSDSYDPDKGVLLL q99zw1_strp1 CRISPR-associated endonuclease Cas1 cas1 SPy_1047 protein protein strp1 Streptococcus pyogenes serotype M1 MAGWRTVVVNTHSKLSYKNNHLIFKDAYKTELIHLSEIDILLLETTDIVLSTMLVKRLVDENVLVIFCDDKRLPTAMLMPFYGRHDSSLQLGKQMSWSETVKSQVWTTIIAQKILNQSCYLGACSYFEKSQSIMDLYHGLENFDPSNREGHAARIYFNTLFGNDFSRDLEHPINAGLDYGYTLLLSMFAREVVVSGCMTQFGLKHANQFNQFNFASDIMEPFRPLVDKIVYENRNQPFPKIKRELFTLFSDTFSYNGKEMYLTNIISDYTKKVVKALNNEGKGVPEFRI csn2_strp1 CRISPR-associated protein Csn2 csn2 M5005_Spy0772 SPy_1049 protein protein strp1 Streptococcus pyogenes serotype M1 MNLNFSLLDEPIPLRGGTILVLEDVCVFSKIVQYCYQYEEDSELKFFDHKMKTIKESEIMLVTDILGFDVNSSTILKLIHADLESQFNEKPEVKSMIDKLVATITELIVFECLENELDLEYDEITILELIKSLGVKVETQSDTIFEKCLEILQIFKYLTKKKLLIFVNSGAFLTKDEVASLQEYISLTNLTVLFLEPRELYDFPQYILDEDYFLITKNMV cas9_strp1 CRISPR-associated endonuclease Cas9/Csn1 cas9 csn1 SPy_1046 SpCas9 SpyCas9 protein protein strp1 Streptococcus pyogenes serotype M1 MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD cas9-cas1-1 3276135 N/A 3276138 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3276139 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3276140 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3276141 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli association association false false false cas1-cas2-1 3276136 N/A 3276138 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3276139 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3276140 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3276141 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false csn2-cas1-1 3276137 N/A 3276138 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3276139 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3276140 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3276141 unspecified role unspecified role prey prey ecolx Escherichia coli association association false false false