DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ haslinger-2015-1 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. DIP original experiment: DIP-265940X Nature 2015 IMEx X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. Only protein-protein interactions imex curation haslinger-2015-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. DIP original experiment: DIP-265933X Nature 2015 IMEx X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. Only protein-protein interactions imex curation haslinger-2015-3 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. DIP original experiment: DIP-265935X Nature 2015 IMEx X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. Only protein-protein interactions imex curation haslinger-2015-4 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. DIP original experiment: DIP-265942X Nature 2015 IMEx X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. Only protein-protein interactions imex curation haslinger-2015-5 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. DIP original experiment: DIP-265936X Nature 2015 IMEx X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. Only protein-protein interactions imex curation haslinger-2015-6 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. DIP original experiment: DIP-265938X Nature 2015 IMEx X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. Only protein-protein interactions imex curation haslinger-2015-7 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. DIP original experiment: DIP-265934X Nature 2015 IMEx X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. Only protein-protein interactions imex curation haslinger-2015-8 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. DIP original experiment: DIP-265941X Nature 2015 IMEx X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. Only protein-protein interactions imex curation haslinger-2015-9 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. DIP original experiment: DIP-265939X Nature 2015 IMEx X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. Only protein-protein interactions imex curation haslinger-2015-10 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. DIP original experiment: DIP-265937X Nature 2015 IMEx X-domain of peptide synthetases recruits oxygenases crucial for glycopeptide biosynthesis. Haslinger K., Peschke M., Brieke C., Maximowitsch E., Cryle MJ. Only protein-protein interactions imex curation o52821_amyor protein protein amyor Amycolatopsis orientalis Nocardia orientalis MTVDDTRAKPRSSVEDVWPLSPLQQGMLYHTALDNDGPDTYTVQTVYGIDGPLDAGLLRASWQALVDRHAALRACFRYVSGAQMVQVIARDAEIPWNETDLSGLPEDIADSEVDRLAAQEVAERLRIEVAPLMKLHLIRLGPESHRLVHTLHHVLVDGWSMPIIHRELAAIYAAGGDASGLPPTVSYRDYLAWLGRQDKDVAIAAWRKELAGLETPTTVAPADPTRVPDIDTVVIELSTQLTDDLYRLARGHDLTLNTVVQGAWAVVLSQLAGRNDVVFGATASGRPADLPGVEAMVGQLLTTLPVRVRLDGERRAVDLFAQLQRDQSALMAHQHLGLQDVQAVVGRGAVFDTLVIYENFPRKGLGRAPDGGLTLLPVKRGRNSSHYPFTLVTGPGEQMPLILDYDRGLFDPAAAESVVGALARVLERLVAEPDILVGRLTLVSEAERALVVDEFNATAGPVPGESVVELFAHRVAEAPDAVAITDAGGANLTYAEVDRASNRLAGYLAGRGVGRGDRVGVAMDRSPELLIAFLAIWKAGAAYVPVDVEYPAERIAFILNDSGVSTVLCTQDTSGVVPENAIVLDAPDTRASIEDCADTAPEIRLYAGDLAYVMYTSGSTGLPKGVAIPHGAVAGLAGDSGWQIGPDDGVLMHATHVFDPSLYAMWVPLVAGGRVLLTEPGVLDAAGVRQAVERGATAVHLTAGSFRALAETSPECFAGLVEIGTGGDVVPAQSVANLRRAQPGLRVRNTYGPTETTLCATWLPIEPGDVIDRELPIGHPMTNRKIYILDAFLRPVPPGVAGELYIAGTGLARGYLDSPGLTADRFVACPFLAGERMYRTGDVARWTRDGEVVFLGRADDQVKIRGYRVELGEVEAVLAAQPGVVEAVVMAREDQPGEKRLVGYFVSDGSDAGPAEIRRQMAQVLPDYMVPIAVVALPGLPVTPNGKVDRRALPAPDLAGRSSEKAPENETEKVLCALFGEILSVDQVGVDDTFNDLGGSSALAMRLIARIREELGADLPIRQLFSSPTPAGLARALAAKSRPALEAAQRPDRVPLTARQLRAWLLARPGEDTADQHVSITLRLGGKLNVPALAAAIGDVAARHEILRTTFPGDAQSVHQHVHDALAIELTPVPATEEDLPGLLAERRESVFDLTQEVPWRCDLFQLSEKDHVLQVKIHRILADDDSMDVFFRDLAAAYGARREGRVPERAPLALQFADFAIWEHRLLEDEQELDSLINEQIVFWRDNLAGIDGETVLPFDRPRSAVPSRRAGTVALRLDADPHARLTAVAEPVGADTLQMVHAALAMLLAKFGAGHDVVIGTTLPRDEELFDLEPMIGPFTRPLALRTDVSGDPTFLEVVTRVQEAAQAAGEHLDVPFERIVELLDLPASLSRHPVFQVGLQVDEEDIDGWDAAELPALRTSVEPGGTEAMELDLAVKLTERFDDDDNSGGIEGALHYATDLFDEATAESVARRLVRVLEQVAEDPQRRISDLDIFLDDFERGRPAIAPARWSGAVPAVVADLAQDGPLGALLLDEQLRPVAPGAVGDLYVTGPAVDAGTAGLPTMPCPFGTPGHQMMHTGLLARKSHTKALVVVGERRRSSASVKTGDFEILLPLRVGGDRPPLFCVHASGGLSWNYEPLLRYLPPNQPVYGVQARGLARTEPLPGSVDEMAADYLEQIRAVQPTGPYHLLGWSLGGRIAQAMAKLLEAEGEQVGLLALLDAYPVYMGRKTTGAASEEEALERRNQQDLELAGQLVKGAAARSRLEAVMRNLWDVGPRHVSTPFAGDILLFVATVDRPGHLPVPVAKDSWKDFTSGAIEAHEIASNHYDMVQPAALGQIGAIVAEKLRSRPDAERTQR q70ay8_actti FHX34_108186 tcp20 protein protein actti actti MSGDGSPPIHTRRQGFDPADELRAAGTLTKISIGSGADAETTWLAAGHAVVRQVLGDHKRFSTRRRFDRRDEIGGTGVFRPRELVGNLMDYDPPEHTRLRHLLTPGFTQRRMRRLAPRIEEIVTDRLDAMEQAGPPADLIELFADEVPGAVLCELIGVPRDDQAMFLQLCHRHLDASLSARKRAAAGEAFARYLVAMMARERKDPGDGFIGSIVAEHGDTITDEELRGVCVQLMLAGDDNVSGMIGLGVLALLRHPEQIAALRGDDQSADRAVDELIRYLTVPYAPTPRTAVEDVMVADQVIKEGETVLCSLPMANRDRALLPDADRLDVTRTPVPHVAFGHGIHHCLGAALTRLQLRIAYTALWRRFPALQLADPAQEIMFRTSTPAYGLTSLLVAW q70az6_actti tcp12 protein protein actti actti MTVDDTRAKPRSSVEDVWPLSPLQEGMLYHTALDGDGPDTYTVQTVYGIDGPLDAGRLRASWQALVDRHAALRACFRYVSGAQMVQVIQRDVELPWRETDLSGLPEDLADGEVARLAEQEMAERLRLEAAPLMKLHLIRLGPQRHRLVHTLHHVLVDGWSMPILHRELAAIYAAGGDASGLPPAVSYRDYLAWLGRQDKESARAAWRDALAGLDTPTTVAPADPARVPDIDTAMVELSPELTDDLLRLAHGHDLTLNTVVQGVWAVVLAQLAGRTDVVFGATASGRPAELAGVEAMVGLLLATLPVRVRLDGRQRVVDMLADLQRDQSALRAHQHLGLQEVQAVVGPGAVFDTLVIYENFPRTGLGGSADDGLVMRPVQKGRDSSHYPFTLVTGPGERMPVILYYDRGLFDQAAATSVLGAIVRVLERLVAEPGVLVGRLTLLGEAERAVVVGDWNATAGPVPGDSMVELFDRRVAAAPDTVAITDADGADLTYAEVDQASNRLAAHLADRGVRRGDRVGVAVERSADLPIVFLAIWKAGAAYVPVDVGYPAERIALILAGSAVSTVICTQATEAAVPQNVPQRATIVLDAPPTRAAVDACAGTAPTFRPSAADLAYVMYTSGSTGVPKGVAVPHGAVAGLAGDAGWRIGPGDGVLMHATHVFDPSLYEMWVPLATGGRVLVAAPGVVDAGGIRQAVARGATAVHLTAGTFRALAEASPDCFAGLREVGTGGDVVPAHTVAHLRRAQPQLRVRNTYGPTETTLCATWKPIEPGAQLGPELPIGRPMTNRRIYILDAFLRPVAPGVAGELYIAGTGLARGYLSRPDLTAERFVACPFLAGERMYRTGDLARWNRDGEVVFLGRADDQVKIRGYRVELAEVEAVLAAQPGVREAVVVAREDRPGERRLVGYVVSDAGELDTEQIRQQMARVLPGYMVPVAVIGLVSLPITANSKVDRRALPAPDLAGHASAKAPESATEKVLCALYAEILGVERVGVDDAFHDLGGSSALAMRLIARIREELGVDLPIRQLFSSPTPAGVARALAAKSRPALEAVTRPERVPLTARQLRAWLLARPSEETRGRHLSVALRLRGRLDVAALEAALRDVAARHEILRTTFPGDAQTVHQHIHDAAPVRLTPVPATEEDLPARLAERGEQLFDLTRDMPWRCELFALSEKEHVLSVTVHRIAADDDSMDVFFRDLAAAYGARRAGRAPERAPLALQFADYAIWEQRLLDGEREQDSLINDQITFWRNHLAGIDQETVLPFDRARPAIPSRRAGTVALRLDAGPHARLAEAVESAGADMPQLVQAALAMLLTRYGAGTDLVIGTTLPRDEDLIDLEPMIGPFARPFPVRTDLSADPTFLEVVARVQEAVREARQHLDVPFEKIPELLALPGSLSRHPVYQVGLQVREEDNGAWDAAELPALRTSVEPTGVEAIELDLAFALTERRNDDDDEDGIEGALHYAADLFDHDTAASLARRLVRVLEQVAEDPGRRISDLDILLDDAERGAPAIAPARWAGAVPAAVADLVAAGPLGALVLDEAQRPVAPGTVGDLWVTGPAVDADTGGRPSVPCPFGTPGHRMLHTGLLARKSPARTLVVVGERRGASASAKTGDYEVLLPLRAAGDRAPLFCIHASGGLSWNYGPLLRHLPPNQPVYGVQARGLARTEPLPGSVEEMAADYVEQIRTVQPTGPYHLLGWSLGGRIAQAMAVLLEADGEKVGLLALLAAYPVYMGRKATGTVTEEAARDEDALERRKQQELELAGQLVQGAGARSRLEAVMRNLWEVGPAHTASPFASDVLLFVASVDRPAHLPVSEAIASWKDFTGGTIEPHEIATNHYEMVQPAALAQIGAIVAEKLRSRPDA c5b3_amyor Cytochrome P450 165B3 cyp165B3 oxyB Vancomycin biosynthesis protein OxyB protein protein amyor Amycolatopsis orientalis Nocardia orientalis MSEDDPRPLHIRRQGLDPADELLAAGALTRVTIGSGADAETHWMATAHAVVRQVMGDHQQFSTRRRWDPRDEIGGKGIFRPRELVGNLMDYDPPEHTRLRRKLTPGFTLRKMQRMAPYIEQIVNDRLDEMERAGSPADLIAFVADKVPGAVLCELVGVPRDDRDMFMKLCHGHLDASLSQKRRAALGDKFSRYLLAMIARERKEPGEGMIGAVVAEYGDDATDEELRGFCVQVMLAGDDNISGMIGLGVLAMLRHPEQIDAFRGDEQSAQRAVDELIRYLTVPYSPTPRIAREDLTLAGQEIKKGDSVICSLPAANRDPALAPDVDRLDVTREPIPHVAFGHGVHHCLGAALARLELRTVFTELWRRFPALRLADPAQDTEFRLTTPAYGLTELMVAW q6zzi8_actti tcp18 FHX34_108188 protein protein actti actti MFEEINVVRAAELHRRDRFDPVPQLRSLMAEGPLTTLGTEESPGGRTAWLATGYDEIRQVLSSDDFSARLLYGGTAAGITWPGFLTQYDPPEHTRLRRMVAPAFAVRRMQKFQPQVERVVQDSLDAIEALGGPVDFVPRFGWSVATTATCDFLGIPRDDQADLARSLHASRTERSGKRRTAAGNKFMTYMNKMTARTRRDPGDDMFGVVVREYGDEITDAELTGVAAFVMGAGADQVARFLAAGAWLMADDPEQFALLREKPDTVPDWLDEVIRYLTTDEKTHPRVATDDVRIGDHLIKAGDTVTCSLLAANRRNFPRPEDRFDITRVRPEHLAFGHGIHHCLGRSLAELVFRTAIPALAHRFPTLRLAEPHREIRLGPPPFDVEALLLDW q70ay6_actti tcp22 protein protein actti actti MRRTVCDPHEDMFALRAHGPLIRIGGDAAVQMGADYLWQAMGYDVVRQILGDHENFTTRLRLTASEPISGEGATKPQELVGQISTYDPPEHTRLRRMLTPEFTVRRVRRLEPAIQELIDDRIDAMEAAGPPVDVQTVFADPVGGGALCELLGIPRDDRNQFMRRVRQNVDLSRGYKARAADSAAFNRYLQSLITRQRKDPDEGFLGMLVRDHGDDITDEELKGVCTALLLGGVETVAGMLGFGVLALLDHPDQRRLLFAGREEADRLVNELLRYLSAVQQPTPRMAVKDVVVDGQQIRAGEYVLCSILMANRDEALTPHPNVLDAEREPAPHIGFGHGIHHCIGAAVARTVLRMAYQTLWRRLPGLRLAIPAGQVKLRNAFIDSPDQLPVTW o52821_amyor-tcp20-1 3275802 N/A 3275812 unspecified role unspecified role unspecified role unspecified role Region 963-1860 Region 963-1860 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3275813 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false tcp12-tcp20-1 3275803 N/A 3275814 unspecified role unspecified role unspecified role unspecified role Region 1047-1511 Region 1047-1511 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3275813 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false tcp12-tcp20-2 3275804 N/A 3275814 unspecified role unspecified role unspecified role unspecified role Region 1047-1511 Region 1047-1511 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3275813 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false cyp165b3-o52821_amyor-1 3275805 N/A 3275815 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3275812 unspecified role unspecified role unspecified role unspecified role Region 1042-1506 Region 1042-1506 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tcp12-tcp18-1 3275806 N/A 3275816 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3275814 unspecified role unspecified role unspecified role unspecified role Region 1047-1511 Region 1047-1511 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tcp12-tcp18-2 3275807 N/A 3275816 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3275814 unspecified role unspecified role unspecified role unspecified role Region 1047-1511 Region 1047-1511 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tcp12-tcp20-3 3275808 N/A 3275814 unspecified role unspecified role unspecified role unspecified role Region 1047-1511 Region 1047-1511 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q70AZ6:p.[Arg1211Ala;Arg1215Ala] R1211A/R1215A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3275813 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false cyp165b3-tcp12-1 3275809 N/A 3275815 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3275814 unspecified role unspecified role unspecified role unspecified role Region 968-1865 Region 968-1865 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tcp12-tcp22-1 3275810 N/A 3275817 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3275814 unspecified role unspecified role unspecified role unspecified role Region 1047-1511 Region 1047-1511 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false tcp12-tcp22-2 3275811 N/A 3275817 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3275814 unspecified role unspecified role unspecified role unspecified role Region 1047-1511 Region 1047-1511 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false