DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ fleurie-2014-1 stree Streptococcus pneumoniae anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Fleurie A., Lesterlin C., Manuse S., Zhao C., Cluzel C., Lavergne JP., Franz-Wachtel M., Macek B., Combet C., Kuru E., VanNieuwenhze MS., Brun YV., Sherratt D., Grangeasse C. DIP original experiment: DIP-264452X Nature 2014 IMEx MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae. Fleurie A., Lesterlin C., Manuse S., Zhao C., Cluzel C., Lavergne JP., Franz-Wachtel M., Macek B., Combet C., Kuru E., VanNieuwenhze MS., Brun YV., Sherratt D., Grangeasse C. Only protein-protein interactions imex curation fleurie-2014-2 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Fleurie A., Lesterlin C., Manuse S., Zhao C., Cluzel C., Lavergne JP., Franz-Wachtel M., Macek B., Combet C., Kuru E., VanNieuwenhze MS., Brun YV., Sherratt D., Grangeasse C. DIP original experiment: DIP-264453X Nature 2014 IMEx MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae. Fleurie A., Lesterlin C., Manuse S., Zhao C., Cluzel C., Lavergne JP., Franz-Wachtel M., Macek B., Combet C., Kuru E., VanNieuwenhze MS., Brun YV., Sherratt D., Grangeasse C. Only protein-protein interactions imex curation fleurie-2014-3 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Fleurie A., Lesterlin C., Manuse S., Zhao C., Cluzel C., Lavergne JP., Franz-Wachtel M., Macek B., Combet C., Kuru E., VanNieuwenhze MS., Brun YV., Sherratt D., Grangeasse C. DIP original experiment: DIP-264454X Nature 2014 IMEx MapZ marks the division sites and positions FtsZ rings in Streptococcus pneumoniae. Fleurie A., Lesterlin C., Manuse S., Zhao C., Cluzel C., Lavergne JP., Franz-Wachtel M., Macek B., Combet C., Kuru E., VanNieuwenhze MS., Brun YV., Sherratt D., Grangeasse C. Only protein-protein interactions imex curation q8dnv9_strr6 Cell division protein FtsZ ftsZ spr1510 protein protein strr6 Streptococcus pneumoniae (strain ATCC BAA-255 / R6) MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAANTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFITAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVDTLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTVMANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEAEEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATNYRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSVVSPVERFEAPISQDEDELDTPPFFKNR mapz_strr6 Mid-cell-anchored protein Z mapZ locZ spr0334 protein protein strr6 Streptococcus pneumoniae (strain ATCC BAA-255 / R6) MSKKRRNRHKKEAQEPQFDFDEAKELTVGQAIRKNEEVEAGVLPEDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEVPSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGFILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSNKTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIVDGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSSQASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAWDFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGYFVGNGAGHADDLDY stkp_strr6 Serine/threonine-protein kinase StkP stkP pkn2 spr1577 Eukaryotic-type Ser/Thr protein kinase protein protein strr6 Streptococcus pneumoniae (strain ATCC BAA-255 / R6) MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVLRTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYIKEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVAFAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAVTIALQHFQNPLPSVIAENSSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYNRRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQAPKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANFEIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSDVIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKATTIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVSKPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVEGMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP mapz-ftsz-1 3272416 N/A 3272419 unspecified role unspecified role prey prey stree Streptococcus pneumoniae N/A 3272420 unspecified role unspecified role bait bait Region 1-158 Region 1-158 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false stree Streptococcus pneumoniae physical association physical association false false false mapz-stkp-1 3272417 N/A 3272420 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli N/A 3272421 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation false false false ftsz-mapz-1 3272418 N/A 3272419 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3272420 unspecified role unspecified role prey prey Region 1-41 Region 1-41 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false