IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ fujita-2015-1 in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Fujita M., Zhu K., Fujita CK., Zhao M., Lam KS., Kurth MJ., Takada YK., Takada Y. The species origins of "recombinant soluble alpha-5/beta-3 integrins" have been taken from other publications by the same group; " The beta3-CHO cells expressed human beta3/hamster alpha 5 hybrid." from pmid:9516430, and pmid:24789099. "Beta3-CHO" cells express human beta3/hamster alpha5 integrins -a hybrid.(see ref 27 leading to Ref 29 of this publication.) The longest Trembl entry has been used for Hamster integrin alpha-5. "Alpha4-CHO" cells express human alpha4/hamster beta1 integrin (see ref 27 referring to pmid:8521812-ref23 of that publication.) The only UniProt entry for "hamster" ITGB1 has been used. Alpha4-K562 cells have been assumed to express Human Alpha4 and beta1 integrins, as the origins of this line cannot be traced. Details of Fibronectin H-120 fragment were obtained from Tonge et al -2012, PMID: 22483961 [The H120 fragment is a construct including type III repeats 12–15 containing the binding site for integrin a4b1.] Figure 6: Integrin a5b1 proteins: it has been assumed that those expressed in K562 cells are of human origin, and those in CHO cells are of mixed human(a5) and hamster (b1) origin, as no details of these proteins/cell lines can be traced. ytakada@ucdavis.edu The Journal of Biological Chemistry 2015 imex curation Recombinant soluble alpha-v/Beta-3;according to Ref 21 (Takagi- 2001 pmid:11323715) gives the sequence of "alpha-v" to be that of P08648 Gene:ITGA5- Integrin alpha-5, rather than that of P06756 -Integrin alpha-V, Gene ITGAV. "Beta3-CHO" cells express human beta3/hamster alpha5 integrins -a hybrid;the longest Trembl entry has been used for Hamster integrin alpha-5. Only protein-protein interactions imex curation fujita-2015-2 in vitro In vitro spa scintillation proximity assay SPA RIA Radio Immuno Assay spa predetermined predetermined participant predetermined Fujita M., Zhu K., Fujita CK., Zhao M., Lam KS., Kurth MJ., Takada YK., Takada Y. The species origins of "recombinant soluble alpha-5/beta-3 integrins" have been taken from other publications by the same group; " The beta3-CHO cells expressed human beta3/hamster alpha 5 hybrid." from pmid:9516430, and pmid:24789099. "Beta3-CHO" cells express human beta3/hamster alpha5 integrins -a hybrid.(see ref 27 leading to Ref 29 of this publication.) The longest Trembl entry has been used for Hamster integrin alpha-5. "Alpha4-CHO" cells express human alpha4/hamster beta1 integrin (see ref 27 referring to pmid:8521812-ref23 of that publication.) The only UniProt entry for "hamster" ITGB1 has been used. Alpha4-K562 cells have been assumed to express Human Alpha4 and beta1 integrins, as the origins of this line cannot be traced. Details of Fibronectin H-120 fragment were obtained from Tonge et al -2012, PMID: 22483961 [The H120 fragment is a construct including type III repeats 12–15 containing the binding site for integrin a4b1.] Figure 6: Integrin a5b1 proteins: it has been assumed that those expressed in K562 cells are of human origin, and those in CHO cells are of mixed human(a5) and hamster (b1) origin, as no details of these proteins/cell lines can be traced. ytakada@ucdavis.edu The Journal of Biological Chemistry 2015 imex curation Recombinant soluble alpha-v/Beta-3;according to Ref 21 (Takagi- 2001 pmid:11323715) gives the sequence of "alpha-v" to be that of P08648 Gene:ITGA5- Integrin alpha-5, rather than that of P06756 -Integrin alpha-V, Gene ITGAV. "Beta3-CHO" cells express human beta3/hamster alpha5 integrins -a hybrid;the longest Trembl entry has been used for Hamster integrin alpha-5. Only protein-protein interactions imex curation itb3_human Integrin beta-3 Platelet membrane glycoprotein IIIa ITGB3 GP3A protein protein human Homo sapiens Human MRARPRPRPLWATVLALGALAGVGVGGPNICTTRGVSSCQQCLAVSPMCAWCSDEALPLGSPRCDLKENLLKDNCAPESIEFPVSEARVLEDRPLSDKGSGDSSQVTQVSPQRIALRLRPDDSKNFSIQVRQVEDYPVDIYYLMDLSYSMKDDLWSIQNLGTKLATQMRKLTSNLRIGFGAFVDKPVSPYMYISPPEALENPCYDMKTTCLPMFGYKHVLTLTDQVTRFNEEVKKQSVSRNRDAPEGGFDAIMQATVCDEKIGWRNDASHLLVFTTDAKTHIALDGRLAGIVQPNDGQCHVGSDNHYSASTTMDYPSLGLMTEKLSQKNINLIFAVTENVVNLYQNYSELIPGTTVGVLSMDSSNVLQLIVDAYGKIRSKVELEVRDLPEELSLSFNATCLNNEVIPGLKSCMGLKIGDTVSFSIEAKVRGCPQEKEKSFTIKPVGFKDSLIVQVTFDCDCACQAQAEPNSHRCNNGNGTFECGVCRCGPGWLGSQCECSEEDYRPSQQDECSPREGQPVCSQRGECLCGQCVCHSSDFGKITGKYCECDDFSCVRYKGEMCSGHGQCSCGDCLCDSDWTGYYCNCTTRTDTCMSSNGLLCSGRGKCECGSCVCIQPGSYGDTCEKCPTCPDACTFKKECVECKKFDRGALHDENTCNRYCRDEIESVKELKDTGKDAVNCTYKNEDDCVVRFQYYEDSSGKSILYVVEEPECPKGPDILVVLLSVMGAILLIGLAALLIWKLLITIHDRKEFAKFEEERARAKWDTANNPLYKEATSTFTNITYRGT fibg_human Fibrinogen gamma chain FGG PRO2061 protein protein human Homo sapiens Human MSWSLHPRNLILYFYALLFLSSTCVAYVATRDNCCILDERFGSYCPTTCGIADFLSTYQTKVDKDLQSLEDILHQVENKTSEVKQLIKAIQLTYNPDESSKPNMIDAATLKSRKMLEEIMKYEASILTHDSSIRYLQEIYNSNNQKIVNLKEKVAQLEAQCQEPCKDTVQIHDITGKDCQDIANKGAKQSGLYFIKPLKANQQFLVYCEIDGSGNGWTVFQKRLDGSVDFKKNWIQYKEGFGHLSPTGTTEFWLGNEKIHLISTQSAIPYALRVELEDWNGRTSTADYAMFKVGPEADKYRLTYAYFAGGDAGDAFDGFDFGDDPSDKFFTSHNGMQFSTWDNDNDKFEGNCAEQDGSGWWMNKCHAGHLNGVYYQGGTYSKASTPNGYDNGIIWATWKTRWYSMKKTTMKIIPFNRLTIGEGQQHHLGGAKQVRPEHPAETEYDSLYPEDDL a0a061ij01_crigr H671_2g5979 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MGSRTPRSPQTPLHAVPLRWGPSRLPPLLPLLLLLWPPPLQVGGFNLDAEAPAVLSGPPGSLFGFSVEFYRPGRDGVSVLVGAPKANTSQPGVLQGGAVYVCPWDTSPAQCTTIQFDSKGSRILESSLSSAKGEEPVEYKSLQWFGATVRAHGSSILACAPLYSWRTEKEPQSDPVGTCYLSTDNFTRILEYAPCRSDFSTAAGQGYCQGGFSAEFTKTGHVVLGGPGSYFWQGQILSATQEQISESYYPEYLINPVQGQLQTRQASSIYDDSYLGYSVAVGEFSGDDTEDFVAGVPKGNLTYGYVTILNGSDIHSLYNVSGEQMASYFGYAVAATDTNGDGLDDLLVGAPLLMERTADGRPQEVGRVYIYLQHPAGIEPTPTLTLTGQDEFGRFGSSLTPLGDLDQDGYNDVAIGAPFGGETQQGVVFIFPGGPGGLNTKPSQILQPLWAAGSTPDFFGSALRGGRDLDGNGYPDLIVGSFGVDKALVYRGRPIISASASLTIFPSMFNPEERSCSLEGNPVSCINLSFCLNASGKHVPNSIGFEVELQLDWQKQKGGIRRALFLASKQATLTQTLLIQNGAREDCREMKIYLRNESEFRDKLSPIHIALNFSLDPKAPMDSHGLRPVLHYQSKSRIEDKAQILLDCGEDNICVPDLQLDVYGEKKHVYLGDKNSLNLTFHAQNLGEGGAYEAELRVTAPLEAEYSGLVRHPGNFSSLSCDYFAVNQSRQLVCDLGNPMKAGTSIWGGLRFTVPHLQDTKKTIQFDFQILSKNLNNSQSNVVSFPLSVEAQAQVSLNGVSKPEAVLFPVSDWYPQDQPQKEGDLGPAVHHVYELINQGPSSISQGVLELSCPQALEGQQLLYVTRVTGLSNCTSNYTPNSQGLELDPESFPHHLQKREAPGRSSTASGPQVLKCPEAKCFMLRCEFGPLHRQESRSLQLHFRVWAKTFLQREHQPFSLQCEAVYEALKMPYQILPRQLPQKKLQVATAVQWTKAEGSNGVPLWIIILAILFGLLLLGLLIYVLYKLGFFKRSLPYGTAMEKAQLKPPATSDA p14555-pro_0000022750 Phospholipase A2, membrane associated Non-pancreatic secretory phospholipase A2 GIIC sPLA2 Group IIA phospholipase A2 Phosphatidylcholine 2-acylhydrolase 2A PLA2G2A PLA2B PLA2L RASF-A protein protein human Homo sapiens Human NLVNFHRMIKLTTGKEAALSYGFYGCHCGVGGRGSPKDATDRCCVTHDCCYKRLEKRGCGTKFLSYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKYQYYSNKHCRGSTPRC 21 144 itb1_human Integrin beta-1 Fibronectin receptor subunit beta VLA-4 subunit beta ITGB1 FNRB MDF2 MSK12 Glycoprotein IIa protein protein human Homo sapiens Human MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK cmpd21 Compound 21 small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) fgg-a0a061ij01_crigr-1 3038841 N/A 3038843 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 N/A 3038844 unspecified role unspecified role bait bait c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false c_terminal_region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecoli Escherichia coli (strain K12) N/A 3038845 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 N/A 3038846 unspecified role unspecified role ancillary ancillary region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false P14555-PRO_0000022750:p.[Arg74Glu;Arg100Glu] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P14555-PRO_0000022750:p.His47Gln mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false P14555-PRO_0000022750:p.[Gly29Ser;Asp48Lys] mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecoli Escherichia coli (strain K12) 3038857 3038851 physical association physical association false false false 1 A/B/C Binding of the B-3 peptide was weaker than that of the B-1 peptide. pla2g2a-itgb3-2 3038841 N/A 3038846 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3038843 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P05106:p.Cys299Ser mutation mutation certain certain sequence position certain certain certain sequence position certain false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3B pla2g2a-itgb3-1 3038841 N/A 3038846 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) N/A 3038847 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) direct interaction direct interaction false false false 3 B/C fgg-a0a061ij01_crigr-3 3038842 N/A 3038843 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 N/A 3038848 inhibitor inhibitor unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) N/A 3038845 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 N/A 3038846 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) 3038883 3038880 physical association physical association false false false 5Bi a0a061ij01_crigr-pla2g2a-1 3038842 N/A 3038843 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 N/A 3038845 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 N/A 3038846 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecoli Escherichia coli (strain K12) 3038890 3038888 physical association physical association false false false 5B