DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ phillips-2014-1 in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. DIP original experiment: DIP-265527X Nature structural & molecular biology 2014 IMEx Internal motions prime cIAP1 for rapid activation. Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. Only protein-protein interactions imex curation phillips-2014-2 in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli weight by comassie molecular weight estimation by coomasie staining weight by comassie Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. DIP original experiment: DIP-265529X Nature structural & molecular biology 2014 IMEx Internal motions prime cIAP1 for rapid activation. Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. Only protein-protein interactions imex curation phillips-2014-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. DIP original experiment: DIP-265530X Nature structural & molecular biology 2014 IMEx Internal motions prime cIAP1 for rapid activation. Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. Only protein-protein interactions imex curation phillips-2014-4 in vitro In vitro bio-layer interferometry bio-layer interferometry bli bli predetermined predetermined participant predetermined Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. DIP original experiment: DIP-265528X Nature structural & molecular biology 2014 IMEx Internal motions prime cIAP1 for rapid activation. Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. Only protein-protein interactions imex curation phillips-2014-5 in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. DIP original experiment: DIP-265531X Nature structural & molecular biology 2014 IMEx Internal motions prime cIAP1 for rapid activation. Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. Only protein-protein interactions imex curation phillips-2014-6 in vitro In vitro light scattering light scattering predetermined predetermined participant predetermined Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. DIP original experiment: DIP-265532X Nature structural & molecular biology 2014 IMEx Internal motions prime cIAP1 for rapid activation. Phillips AH., Schoeffler AJ., Matsui T., Weiss TM., Blankenship JW., Zobel K., Giannetti AM., Dueber EC., Fairbrother WJ. Only protein-protein interactions imex curation p61077-1 Ubiquitin-conjugating enzyme E2 D3 UBE2D3 UBC5C UBCH5C (E3-independent) E2 ubiquitin-conjugating enzyme D3 E2 ubiquitin-conjugating enzyme D3 Ubiquitin carrier protein D3 Ubiquitin-conjugating enzyme E2(17)KB 3 Ubiquitin-conjugating enzyme E2-17 kDa 3 Ubiquitin-protein ligase D3 protein protein human Homo sapiens Human MALKRINKELSDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSICLDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDRDKYNRISREWTQKYAM q13490-1 Baculoviral IAP repeat-containing protein 2 BIRC2 API1 MIHB RNF48 Cellular inhibitor of apoptosis 1 IAP homolog B Inhibitor of apoptosis protein 2 RING finger protein 48 RING-type E3 ubiquitin transferase BIRC2 TNFR2-TRAF-signaling complex protein 2 protein protein human Homo sapiens Human MHKTASQRLFPGPSYQNIKSIMEDSTILSDWTNSNKQKMKYDFSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKLGDSPIQKHKQLYPSCSFIQNLVSASLGSTSKNTSPMRNSFAHSLSPTLEHSSLFSGSYSSLSPNPLNSRAVEDISSSRTNPYSYAMSTEEARFLTYHMWPLTFLSPSELARAGFYYIGPGDRVACFACGGKLSNWEPKDDAMSEHRRHFPNCPFLENSLETLRFSISNLSMQTHAARMRTFMYWPSSVPVQPEQLASAGFYYVGRNDDVKCFCCDGGLRCWESGDDPWVEHAKWFPRCEFLIRMKGQEFVDEIQGRYPHLLEQLLSTSDTTGEENADPPIIHFGPGESSSEDAVMMNTPVVKSALEMGFNRDLVKQTVQSKILTTGENYKTVNDIVSALLNAEDEKREEEKEKQAEEMASDDLSLIRKNRMALFQQLTCVLPILDNLLKANVINKQEHDIIKQKTQIPLQARELIDTILVKGNAAANIFKNCLKEIDSTLYKNLFVDKNMKYIPTEDVSGLSLEEQLRRLQEERTCKVCMDKEVSVVFIPCGHLVVCQECAPSLRKCPICRGIIKGTVRTFLS ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG birc2-ube2d3-1 3271335 N/A 3271341 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3271342 unspecified role unspecified role bait bait Region 266-362/373-618 Region 266-362/373-618 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false birc2-1 3271336 N/A 3271342 unspecified role unspecified role unspecified role unspecified role Region 266-362/373-618 Region 266-362/373-618 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false birc2-2 3271337 N/A 3271342 unspecified role unspecified role unspecified role unspecified role Region 266-362/373-618 Region 266-362/373-618 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ub-birc2-1 3271338 N/A 3271343 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3271342 unspecified role unspecified role prey prey Region 266-362/373-618 Region 266-362/373-618 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q13490-1:p.[Met402Ala;Phe404Ala] M402A/F404A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false birc2-3 3271339 N/A 3271342 unspecified role unspecified role unspecified role unspecified role Region 266-362/373-618 Region 266-362/373-618 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false birc2-4 3271340 N/A 3271342 unspecified role unspecified role unspecified role unspecified role Region 266-362/373-618 Region 266-362/373-618 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false