IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ gltekin-2014-2 human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Gültekin Y., Eren E., Özören N., Gültekin Y., Eren E., Özören N. Results obtained using NLRP3, Caspase_1, Caspase_5 and NOD1/2 containing plasmids have not been included as the origins of the plasmids are not stated, and the authors did not reply to a request for information. nesrin.ozoren@boun.edu.tr Journal of innate immunity 2014 imex curation Only protein-protein interactions gltekin-2014-3 human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Gültekin Y., Eren E., Özören N., Gültekin Y., Eren E., Özören N. Results obtained using NLRP3, Caspase_1, Caspase_5 and NOD1/2 containing plasmids have not been included as the origins of the plasmids are not stated, and the authors did not reply to a request for information. nesrin.ozoren@boun.edu.tr Journal of innate immunity 2014 imex curation Only protein-protein interactions gltekin-2014-4 human-293ft Homo sapiens HEK293FT cell line 293ft HEK293FT cell line anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Gültekin Y., Eren E., Özören N., Gültekin Y., Eren E., Özören N. Results obtained using NLRP3, Caspase_1, Caspase_5 and NOD1/2 containing plasmids have not been included as the origins of the plasmids are not stated, and the authors did not reply to a request for information. nesrin.ozoren@boun.edu.tr Journal of innate immunity 2014 imex curation Only protein-protein interactions asc_human Apoptosis-associated speck-like protein containing a CARD PYD and CARD domain-containing protein Target of methylation-induced silencing 1 Caspase recruitment domain-containing protein 5 ASC CARD5 TMS1 PYCARD protein protein human Homo sapiens Human MGRARDAILDALENLTAEELKKFKLKLLSVPLREGYGRIPRGALLSMDALDLTDKLVSFYLETYGAELTANVLRDMGLQEMAGQLQAATHQGSGAAPAGIQAPPQSAAKPGLHFIDQHRAALIARVTNVEWLLDALYGKVLTDEQYQAVRAEPTNPSKMRKLFSFTPAWNWTCKDLLLQALRESQSYLVEDLERS nlrc3_human NLR family CARD domain-containing protein 3 NLRC3 Nucleotide-binding oligomerization domain protein 3 Caterpiller protein 16.2 CARD15-like protein NOD3 NACHT, LRR and CARD domains-containing protein 3 protein protein human Homo sapiens Human MRKQEVRTGREAGQGHGTGSPAEQVKALMDLLAGKGSQGSQAPQALDRTPDAPLGPCSNDSRIQRHRKALLSKVGGGPELGGPWHRLASLLLVEGLTDLQLREHDFTQVEATRGGGHPARTVALDRLFLPLSRVSVPPRVSITIGVAGMGKTTLVRHFVRLWAHGQVGKDFSLVLPLTFRDLNTHEKLCADRLICSVFPHVGEPSLAVAVPARALLILDGLDECRTPLDFSNTVACTDPKKEIPVDHLITNIIRGNLFPEVSIWITSRPSASGQIPGGLVDRMTEIRGFNEEEIKVCLEQMFPEDQALLGWMLSQVQADRALYLMCTVPAFCRLTGMALGHLWRSRTGPQDAELWPPRTLCELYSWYFRMALSGEGQEKGKASPRIEQVAHGGRKMVGTLGRLAFHGLLKKKYVFYEQDMKAFGVDLALLQGAPCSCFLQREETLASSVAYCFTHLSLQEFVAAAYYYGASRRAIFDLFTESGVSWPRLGFLTHFRSAAQRAMQAEDGRLDVFLRFLSGLLSPRVNALLAGSLLAQGEHQAYRTQVAELLQGCLRPDAAVCARAINVLHCLHELQHTELARSVEEAMESGALARLTGPAHRAALAYLLQVSDACAQEANLSLSLSQGVLQSLLPQLLYCRKLRLDTNQFQDPVMELLGSVLSGKDCRIQKISLAENQISNKGAKALARSLLVNRSLTSLDLRGNSIGPQGAKALADALKINRTLTSLSLQGNTVRDDGARSMAEALASNRTLSMLHLQKNSIGPMGAQRMADALKQNRSLKELMFSSNSIGDGGAKALAEALKVNQGLESLDLQSNSISDAGVAALMGALCTNQTLLSLSLRENSISPEGAQAIAHALCANSTLKNLDLTANLLHDQGARAIAVAVRENRTLTSLHLQWNFIQAGAAQALGQALQLNRSLTSLDLQENAIGDDGACAVARALKVNTALTALYLQVASIGASGAQVLGEALAVNRTLEILDLRGNAIGVAGAKALANALKVNSSLRRLNLQENSLGMDGAICIATALSGNHRLQHINLQGNHIGDSGARMISEAIKTNAPTCTVEM nlrc3-pycard-1 2960075 N/A 2960078 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed N/A 2960079 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false 2A pycard-nlrc3-1 2960076 N/A 2960078 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n_terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 2960079 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed n_terminus egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false colocalization colocalization false false false 2B NLRC3 and ASC colocalization is only partial. pycard-nlrc3-2 2960077 N/A 2960078 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed n_terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false N/A 2960079 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false physical association physical association false false false Supp fig 1