DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ wysoczaski-2014-1 in vitro In vitro peptide array peptide array predetermined predetermined participant predetermined Wysoczański P., Schneider C., Xiang S., Munari F., Trowitzsch S., Wahl MC., Lührmann R., Becker S., Zweckstetter M. DIP original experiment: DIP-262212X Nature structural & molecular biology 2014 IMEx Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex. Wysoczański P., Schneider C., Xiang S., Munari F., Trowitzsch S., Wahl MC., Lührmann R., Becker S., Zweckstetter M. Only protein-protein interactions imex curation wysoczaski-2014-2 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Wysoczański P., Schneider C., Xiang S., Munari F., Trowitzsch S., Wahl MC., Lührmann R., Becker S., Zweckstetter M. DIP original experiment: DIP-262207X Nature structural & molecular biology 2014 IMEx Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex. Wysoczański P., Schneider C., Xiang S., Munari F., Trowitzsch S., Wahl MC., Lührmann R., Becker S., Zweckstetter M. Only protein-protein interactions imex curation wysoczaski-2014-3 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Wysoczański P., Schneider C., Xiang S., Munari F., Trowitzsch S., Wahl MC., Lührmann R., Becker S., Zweckstetter M. DIP original experiment: DIP-262209X Nature structural & molecular biology 2014 IMEx Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex. Wysoczański P., Schneider C., Xiang S., Munari F., Trowitzsch S., Wahl MC., Lührmann R., Becker S., Zweckstetter M. Only protein-protein interactions imex curation wysoczaski-2014-4 in vitro In vitro peptide array peptide array predetermined predetermined participant predetermined Wysoczański P., Schneider C., Xiang S., Munari F., Trowitzsch S., Wahl MC., Lührmann R., Becker S., Zweckstetter M. DIP original experiment: DIP-262213X Nature structural & molecular biology 2014 IMEx Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex. Wysoczański P., Schneider C., Xiang S., Munari F., Trowitzsch S., Wahl MC., Lührmann R., Becker S., Zweckstetter M. Only protein-protein interactions imex curation wysoczaski-2014-5 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Wysoczański P., Schneider C., Xiang S., Munari F., Trowitzsch S., Wahl MC., Lührmann R., Becker S., Zweckstetter M. DIP original experiment: DIP-262211X Nature structural & molecular biology 2014 IMEx Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex. Wysoczański P., Schneider C., Xiang S., Munari F., Trowitzsch S., Wahl MC., Lührmann R., Becker S., Zweckstetter M. Only protein-protein interactions imex curation wysoczaski-2014-6 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Wysoczański P., Schneider C., Xiang S., Munari F., Trowitzsch S., Wahl MC., Lührmann R., Becker S., Zweckstetter M. DIP original experiment: DIP-262208X Nature structural & molecular biology 2014 IMEx Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex. Wysoczański P., Schneider C., Xiang S., Munari F., Trowitzsch S., Wahl MC., Lührmann R., Becker S., Zweckstetter M. Only protein-protein interactions imex curation wysoczaski-2014-7 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Wysoczański P., Schneider C., Xiang S., Munari F., Trowitzsch S., Wahl MC., Lührmann R., Becker S., Zweckstetter M. DIP original experiment: DIP-262210X Nature structural & molecular biology 2014 IMEx Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex. Wysoczański P., Schneider C., Xiang S., Munari F., Trowitzsch S., Wahl MC., Lührmann R., Becker S., Zweckstetter M. Only protein-protein interactions imex curation cwc26_yeast Pre-mRNA-splicing factor CWC26 YGL174W G1642 BUD13 SLC7 Complexed with CEF1 protein 26 Bud site selection protein 13 Synthetic lethal with CLF1 protein 7 CWC26 protein protein yeast Saccharomyces cerevisiae Baker's yeast MALHQYLSETYGPTKPKNKTKKKKKESKSDANSDKTSLIVKERLSTLQQEQEKSGVASFSKFDKQKSKNIWKNLETNELSHAITHPSASSITGNESKNDLKEIRAQEPLVTVADKSKTRKTIYRDAQGHKIQEDSKIDDSSFSRSKYEDEKAAEREQYLKNLNMGDVQKLGINVDAHDKKKNQTASSLTIEDPAITFTHDKERTVKTSLLGRKLYDKPAPENRFAIMPGSRWDGVHRSNGFEEKWFAKQNEINEKKVQSYTLQEDY 1584B390121325CD ist3_yeast U2 snRNP component IST3 IST3 SNU17 YIR005W YIB5W U2 snRNP protein SNU17 Increased sodium tolerance protein 3 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNKIQQINDKELQSGILSPHQSWHNEYKDNAYIYIGNLNRELTEGDILTVFSEYGVPVDVILSRDENTGESQGFAYLKYEDQRSTILAVDNLNGFKIGGRALKIDHTFYRPKRSLQKYYEAVKEELDRDIVSKNNAEKLILAKKDQPN 9D51C3043AB823D2 pml1_yeast Pre-mRNA leakage protein 1 L1591 YLR016C PML1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFHRRKRPYNTRNYGHDDKKFKSQYIDIMPDFSPSGLLELESNNKEGIALKHVEPQDAISPDNYMDMLGLEARDRTMYELVIYRKNDKDKGPWKRYDLNGRSCYLVGRELGHSLDTDLDDRTEIVVADIGIPEETSSKQHCVIQFRNVRGILKCYVMDLDSSNGTCLNNVVIPGARYIELRSGDVLTLSEFEEDNDYELIFMNV DA39B4465EFFD378 bud13p-snu17p-1 3269897 N/A 3269904 unspecified role unspecified role bait bait Region 215-245 Region 215-245 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3269905 unspecified role unspecified role prey prey Region 25-138 Region 25-138 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false bud13p-pml1p-1 3269898 N/A 3269904 unspecified role unspecified role unspecified role unspecified role Region 215-245 Region 215-245 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3269906 unspecified role unspecified role unspecified role unspecified role Region 22-42 Region 22-42 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3269905 unspecified role unspecified role unspecified role unspecified role Region 25-138 Region 25-138 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false pml1p-snu17p-1 3269899 N/A 3269906 unspecified role unspecified role unspecified role unspecified role Region 22-42 Region 22-42 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3269905 unspecified role unspecified role unspecified role unspecified role Region 25-138 Region 25-138 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pml1p-snu17p-2 3269900 N/A 3269906 unspecified role unspecified role bait bait Region 22-42 Region 22-42 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3269905 unspecified role unspecified role prey prey Region 25-138 Region 25-138 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false pml1p-snu17p-3 3269901 N/A 3269906 unspecified role unspecified role unspecified role unspecified role Region 22-42 Region 22-42 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q07930:p.Phe32Ala F32A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q07930:p.Leu38Ala L38A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3269905 unspecified role unspecified role unspecified role unspecified role Region 25-138 Region 25-138 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false bud13p-snu17p-2 3269902 N/A 3269904 unspecified role unspecified role unspecified role unspecified role Region 215-245 Region 215-245 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3269905 unspecified role unspecified role unspecified role unspecified role Region 25-138 Region 25-138 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false bud13p-snu17p-3 3269903 N/A 3269904 unspecified role unspecified role unspecified role unspecified role Region 215-245 Region 215-245 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P46947:p.Trp232Ala W232A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P46947:p.Arg237Ala R237A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P46947:p.Phe224Ala F224A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P46947:p.Val235Ala V235A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3269905 unspecified role unspecified role unspecified role unspecified role Region 25-138 Region 25-138 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false