DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ itou-2014-1 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Itou H., Muramatsu S., Shirakihara Y., Araki H. DIP original experiment: DIP-261994X Structure (London, England : 1993) 2014 IMEx Crystal structure of the homology domain of the eukaryotic DNA replication proteins Sld3/Treslin. Itou H., Muramatsu S., Shirakihara Y., Araki H. Only protein-protein interactions imex curation itou-2014-2 in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Itou H., Muramatsu S., Shirakihara Y., Araki H. DIP original experiment: DIP-261998X Structure (London, England : 1993) 2014 IMEx Crystal structure of the homology domain of the eukaryotic DNA replication proteins Sld3/Treslin. Itou H., Muramatsu S., Shirakihara Y., Araki H. Only protein-protein interactions imex curation itou-2014-3 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by staining molecular weight estimation by staining weight by staining Itou H., Muramatsu S., Shirakihara Y., Araki H. DIP original experiment: DIP-261996X Structure (London, England : 1993) 2014 IMEx Crystal structure of the homology domain of the eukaryotic DNA replication proteins Sld3/Treslin. Itou H., Muramatsu S., Shirakihara Y., Araki H. Only protein-protein interactions imex curation itou-2014-4 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Itou H., Muramatsu S., Shirakihara Y., Araki H. DIP original experiment: DIP-261995X Structure (London, England : 1993) 2014 IMEx Crystal structure of the homology domain of the eukaryotic DNA replication proteins Sld3/Treslin. Itou H., Muramatsu S., Shirakihara Y., Araki H. Only protein-protein interactions imex curation itou-2014-5 in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Itou H., Muramatsu S., Shirakihara Y., Araki H. DIP original experiment: DIP-261997X Structure (London, England : 1993) 2014 IMEx Crystal structure of the homology domain of the eukaryotic DNA replication proteins Sld3/Treslin. Itou H., Muramatsu S., Shirakihara Y., Araki H. Only protein-protein interactions imex curation cdc45_yeast Cell division control protein 45 CDC45 YLR103C L8004.11 SLD4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MYYGISQFSEAYNKILRNSSSHSSCQLVIFVSCLNIDALCATKMLSLLFKKQLVQSQIVPIFGYSELRRHYSQLDDNINSLLLVGFGGVIDLEAFLEIDPQEYVIDTDEKSGEQSFRRDIYVLDAHRPWNLDNIFGSQIIQCFDDGTVDDTLGEQKEAYYKLLELDEESGDDELSGDENDNNGGDDEATDADEVTDEDEEDEDETISNKRGNSSIGPNDLSKRKQRKKQIHEYEGVLEEYYSQGTTVVNSISAQIYSLLSAIGETNLSNLWLNILGTTSLDIAYAQVYNRLYPLLQDEVKRLTPSSRNSVKTPDTLTLNIQPDYYLFLLRHSSLYDSFYYSNYVNAKLSLWNENGKKRLHKMFARMGIPLSTAQETWLYMDHSIKRELGIIFDKNLDRYGLQDIIRDGFVRTLGYRGSISASEFVEALTALLEVGNSTDKDSVKINNDNNDDTDGEEEEDNSAQKLTNLRKRWVSNFWLSWDALDDRKVELLNRGIQLAQDLQRAIFNTGVAILEKKLIKHLRIYRLCVLQDGPDLDLYRNPLTLLRLGNWLIECCAESEDKQLLPMVLASIDENTDTYLVAGLTPRYPRGLDTIHTKKPILNNFSMAFQQITAETDAKVRIDNFESSIIEIRREDLSPFLEKLTLSGLL 6500DDD62EC4E8EE sld3_yeast DNA replication regulator SLD3 SLD3 G2980 YGL113W protein protein yeast Saccharomyces cerevisiae Baker's yeast METWEVIASVKEATKGLDLSLDHPLIIKSEDVPSNILQLLQQKNRRQLKHICMKSRKEYFLLEEYGPGFWVKWPYNYFNGYSLPERRTEVVTTVERERAKRETLKTWDELKFKELLHLWSEEPKGSCKLEKDKDLKLDMNPPDMKGESKINDYYSDPKEYIESKYYDALFSIHTPLAYFVKSNLVRLKNTCRTKYGSDSYKIAYQAMLQKFLLSIVQFKDRHDNRLLLEPFSSPIADEKRKNCLTKFVIQDENKNSSTIADLCVVLKSREIKLQILLLLEIIGLNDLDWNFRDFEKKYKLKLKKRSLNLTKKGLVRRRSKKKTSEKDKGIERITTSLDYCEQLDLYLDRACILDILLSSETPNPDAIEASNGTIQEHKKNILDKSKEASLVGFINYVLIPYFNKKVPHAVEFIIQKLKGPSMRPKRALKKVNDSTNVSSPNTVETYNRLSTSQRASRSSIINSVPSSPALRRVDANLFSRKSIASPTPELLNSRTNSNLNEFLESETRSLKRPSQLGRTKSDLTMNHLQKRQFSVSDLSTTRVPNSSTITLKTPFSHSTINAYKTMNNSFRRVGKRKDINETIRLHERVDSEENVQVQATPAVKKRTVTPNKKAQLQSIIESPLNFKDDDTHEGRKNTSNITSTPTNKPPENSSKRRVRRRLFAPEST 6E59D0DB3C082C28 sld3-cdc45-4 3269018 N/A 3269023 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3269024 unspecified role unspecified role bait bait Region 148-430 Region 148-430 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 301-330 Region 301-330 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli physical association physical association false false false sld3-cdc45-5 3269019 N/A 3269023 unspecified role unspecified role prey prey Q08032:p.[Asp91Asn;Asp124Asn;Asp149_Asp150delinsAsnAsn] D2N mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3269024 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region 296-332 Region 296-332 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cdc45-sld3-8 3269020 N/A 3269023 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3269024 unspecified role unspecified role unspecified role unspecified role Region 148-430 Region 148-430 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false sld3-cdc45-6 3269021 N/A 3269023 unspecified role unspecified role prey prey Q08032:p.[Asp91Asn;Asp124Asn;Asp149_Asp150delinsAsnAsn] D2N mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3269024 unspecified role unspecified role bait bait Region 148-430 Region 148-430 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli physical association physical association false false false sld3-cdc45-7 3269022 N/A 3269023 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3269024 unspecified role unspecified role bait bait Region 296-332 Region 296-332 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 301-330 Region 301-330 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli direct interaction direct interaction false false false