DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ amorim-2014-1 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Amorim NA., da Silva EM., de Castro RO., da Silva-Januário ME., Mendonça LM., Bonifacino JS., da Costa LJ., daSilva LL. DIP original experiment: DIP-266789X The Journal of biological chemistry 2014 IMEx Interaction of HIV-1 Nef protein with the host protein Alix promotes lysosomal targeting of CD4 receptor. Amorim NA., da Silva EM., de Castro RO., da Silva-Januário ME., Mendonça LM., Bonifacino JS., da Costa LJ., daSilva LL. Only protein-protein interactions imex curation amorim-2014-2 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Amorim NA., da Silva EM., de Castro RO., da Silva-Januário ME., Mendonça LM., Bonifacino JS., da Costa LJ., daSilva LL. DIP original experiment: DIP-266794X The Journal of biological chemistry 2014 IMEx Interaction of HIV-1 Nef protein with the host protein Alix promotes lysosomal targeting of CD4 receptor. Amorim NA., da Silva EM., de Castro RO., da Silva-Januário ME., Mendonça LM., Bonifacino JS., da Costa LJ., daSilva LL. Only protein-protein interactions imex curation amorim-2014-3 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Amorim NA., da Silva EM., de Castro RO., da Silva-Januário ME., Mendonça LM., Bonifacino JS., da Costa LJ., daSilva LL. DIP original experiment: DIP-266793X The Journal of biological chemistry 2014 IMEx Interaction of HIV-1 Nef protein with the host protein Alix promotes lysosomal targeting of CD4 receptor. Amorim NA., da Silva EM., de Castro RO., da Silva-Januário ME., Mendonça LM., Bonifacino JS., da Costa LJ., daSilva LL. Only protein-protein interactions imex curation amorim-2014-4 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Amorim NA., da Silva EM., de Castro RO., da Silva-Januário ME., Mendonça LM., Bonifacino JS., da Costa LJ., daSilva LL. DIP original experiment: DIP-266791X The Journal of biological chemistry 2014 IMEx Interaction of HIV-1 Nef protein with the host protein Alix promotes lysosomal targeting of CD4 receptor. Amorim NA., da Silva EM., de Castro RO., da Silva-Januário ME., Mendonça LM., Bonifacino JS., da Costa LJ., daSilva LL. Only protein-protein interactions imex curation amorim-2014-5 human Homo sapiens Human bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Amorim NA., da Silva EM., de Castro RO., da Silva-Januário ME., Mendonça LM., Bonifacino JS., da Costa LJ., daSilva LL. DIP original experiment: DIP-266795X The Journal of biological chemistry 2014 IMEx Interaction of HIV-1 Nef protein with the host protein Alix promotes lysosomal targeting of CD4 receptor. Amorim NA., da Silva EM., de Castro RO., da Silva-Januário ME., Mendonça LM., Bonifacino JS., da Costa LJ., daSilva LL. Only protein-protein interactions imex curation amorim-2014-6 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Amorim NA., da Silva EM., de Castro RO., da Silva-Januário ME., Mendonça LM., Bonifacino JS., da Costa LJ., daSilva LL. DIP original experiment: DIP-266790X The Journal of biological chemistry 2014 IMEx Interaction of HIV-1 Nef protein with the host protein Alix promotes lysosomal targeting of CD4 receptor. Amorim NA., da Silva EM., de Castro RO., da Silva-Januário ME., Mendonça LM., Bonifacino JS., da Costa LJ., daSilva LL. Only protein-protein interactions imex curation amorim-2014-7 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Amorim NA., da Silva EM., de Castro RO., da Silva-Januário ME., Mendonça LM., Bonifacino JS., da Costa LJ., daSilva LL. DIP original experiment: DIP-266792X The Journal of biological chemistry 2014 IMEx Interaction of HIV-1 Nef protein with the host protein Alix promotes lysosomal targeting of CD4 receptor. Amorim NA., da Silva EM., de Castro RO., da Silva-Januário ME., Mendonça LM., Bonifacino JS., da Costa LJ., daSilva LL. Only protein-protein interactions imex curation q90vu7_9hiv1 Protein Nef nef 3'ORF Negative factor protein protein 9hiv1 Human immunodeficiency virus 1 MGGKWSKSSVIGWPAVRERMRRAEPAADGVGAVSRDLEKHGAITSSNTAANNAACAWLEAQEEEEVGFPVTPQVPLRPMTYKAAVDLSHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGYFPDWQNYTPGPGVRYPLTFGWCYKLVPVEPDKVEEANKGENTSLLHPVSLHGMDDPEREVLEWRFDSRLAFHHVARELHPEYFKNC mint pdc6i_human Programmed cell death 6-interacting protein PDCD6IP AIP1 KIAA1375 ALIX ALG-2-interacting protein 1 Hp95 ALG-2-interacting protein X protein protein human Homo sapiens Human MATFISVQLKKTSEVDLAKPLVKFIQQTYPSGGEEQAQYCRAAEELSKLRRAAVGRPLDKHEGALETLLRYYDQICSIEPKFPFSENQICLTFTWKDAFDKGSLFGGSVKLALASLGYEKSCVLFNCAALASQIAAEQNLDNDEGLKIAAKHYQFASGAFLHIKETVLSALSREPTVDISPDTVGTLSLIMLAQAQEVFFLKATRDKMKDAIIAKLANQAADYFGDAFKQCQYKDTLPKEVFPVLAAKHCIMQANAEYHQSILAKQQKKFGEEIARLQHAAELIKTVASRYDEYVNVKDFSDKINRALAAAKKDNDFIYHDRVPDLKDLDPIGKATLVKSTPVNVPISQKFTDLFEKMVPVSVQQSLAAYNQRKADLVNRSIAQMREATTLANGVLASLNLPAAIEDVSGDTVPQSILTKSRSVIEQGGIQTVDQLIKELPELLQRNREILDESLRLLDEEEATDNDLRAKFKERWQRTPSNELYKPLRAEGTNFRTVLDKAVQADGQVKECYQSHRDTIVLLCKPEPELNAAIPSANPAKTMQGSEVVNVLKSLLSNLDEVKKEREGLENDLKSVNFDMTSKFLTALAQDGVINEEALSVTELDRVYGGLTTKVQESLKKQEGLLKNIQVSHQEFSKMKQSNNEANLREEVLKNLATAYDNFVELVANLKEGTKFYNELTEILVRFQNKCSDIVFARKTERDELLKDLQQSIAREPSAPSIPTPAYQSSPAGGHAPTPPTPAPRTMPPTKPQPPARPPPPVLPANRAPSATAPSPVGAGTAAPAPSQTPGSAPPPQAQGPPYPTYPGYPGYCQMPMPMGYNPYAYGQYNMPYPPVYHQSPGQAPYPGPQQPSYPFPQPPQQSYYPQQ q88019_siv nef protein protein siv Simian immunodeficiency virus MGGAISMRRSRPSGDLRQRLLRARGETYGRLLGEVEDGYSQSPGGLDKGLSSLSCEGQKYNQGQYMNTPWRNPAEEREKLAYRKQNMDDIDE o90156_9hiv1 Protein Nef 3'ORF Negative factor nef protein protein 9hiv1 Human immunodeficiency virus 1 MGGKLSKCGGVGWSTVRERMRRAEPAADREPAVGVGAASRDLGKHGAITSSNTAATNADCAWLEAQQEEEEVGFPVRPQVPLRPMTYKAALDLSHFLKEKGGLEGLIYSQKRQDILDLWVYNTQGYFPDWQNYTPGPGIRYPLTFGWCFKLVPVDPEKVEAANEGENNCLLHPISLHGMEDPEKEVLQWKFDSRLAYHHMARELHPEYYKNC q306m3_9hiv1 Protein Nef 3'ORF Negative factor nef protein protein 9hiv1 Human immunodeficiency virus 1 MGGKWSKRSAGGWSAVRKRMEQAEPAADGVGAVSRDLEKYGAITSSNTATNNADCAWLEAQEEEEVGFPVRPQVPLRPMTYKAAVDISHFLKEKGGLEGLIHSQRRQDILDLWIYHTQGFFPDWQNYTPGPGIRYPLTFGWCFKLVPVEAEQVEEANTGENNSLLHPMSLHGMEDAEREVLQWKFDSRLAFHHMARELHPEYYKDC pdcd6ip-nef-1 3268780 N/A 3268787 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3268788 unspecified role unspecified role bait bait Region 1-359 Region 1-359 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 360-716 Region 360-716 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false pdcd6ip-nef-2 3268781 N/A 3268789 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3268788 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-717 Region 1-717 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pdcd6ip-nef-3 3268782 N/A 3268790 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3268788 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-717 Region 1-717 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pdcd6ip-nef-4 3268783 N/A 3268787 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3268788 unspecified role unspecified role bait bait Region 1-359 Region 1-359 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 360-716 Region 360-716 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false nef-pdcd6ip-1 3268784 N/A 3268787 unspecified role unspecified role unspecified role unspecified role nVenus-tag nVenus-tag n-venus n-venus c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3268788 unspecified role unspecified role unspecified role unspecified role cVenus-tag cVenus-tag c-venus c-venus n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false pdcd6ip-nef-5 3268785 N/A 3268787 unspecified role unspecified role prey prey V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3268788 unspecified role unspecified role bait bait Region 1-167 Region 1-167 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false pdcd6ip-nef-6 3268786 N/A 3268791 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3268788 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-717 Region 1-717 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false