DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ jackson-2014-1 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jackson RN., Golden SM., van Erp PB., Carter J., Westra ER., Brouns SJ., van der Oost J., Terwilliger TC., Read RJ., Wiedenheft B. DIP original experiment: DIP-264625X Science (New York, N.Y.) 2014 IMEx Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. Jackson RN., Golden SM., van Erp PB., Carter J., Westra ER., Brouns SJ., van der Oost J., Terwilliger TC., Read RJ., Wiedenheft B. Only protein-protein interactions imex curation jackson-2014-2 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Jackson RN., Golden SM., van Erp PB., Carter J., Westra ER., Brouns SJ., van der Oost J., Terwilliger TC., Read RJ., Wiedenheft B. DIP original experiment: DIP-264627X Science (New York, N.Y.) 2014 IMEx Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. Jackson RN., Golden SM., van Erp PB., Carter J., Westra ER., Brouns SJ., van der Oost J., Terwilliger TC., Read RJ., Wiedenheft B. Only protein-protein interactions imex curation jackson-2014-3 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Jackson RN., Golden SM., van Erp PB., Carter J., Westra ER., Brouns SJ., van der Oost J., Terwilliger TC., Read RJ., Wiedenheft B. DIP original experiment: DIP-264626X Science (New York, N.Y.) 2014 IMEx Structural biology. Crystal structure of the CRISPR RNA-guided surveillance complex from Escherichia coli. Jackson RN., Golden SM., van Erp PB., Carter J., Westra ER., Brouns SJ., van der Oost J., Terwilliger TC., Read RJ., Wiedenheft B. Only protein-protein interactions imex curation cas6_ecoli CRISPR system Cascade subunit CasE casE CasE endoRNase cas6e crRNA endonuclease ygcH JW2726 b2756 protein protein ecoli Escherichia coli (strain K12) MYLSKVIIARAWSRDLYQLHQGLWHLFPNRPDAARDFLFHVEKRNTPEGCHVLLQSAQMPVSTAVATVIKTKQVEFQLQVGVPLYFRLRANPIKTILDNQKRLDSKGNIKRCRVPLIKEAEQIAWLQRKLGNAARVEDVHPISERPQYFSGDGKSGKIQTVCFEGVLTINDAPALIDLVQQGIGPAKSMGCGLLSLAPL cas5_ecoli CRISPR system Cascade subunit CasD casD cas5 ygcI b2757 JW5844 protein protein ecoli Escherichia coli (strain K12) MRSYLILRLAGPMQAWGQPTFEGTRPTGRFPTRSGLLGLLGACLGIQRDDTSSLQALSESVQFAVRCDELILDDRRVSVTGLRDYHTVLGAREDYRGLKSHETIQTWREYLCDASFTVALWLTPHATMVISELEKAVLKPRYTPYLGRRSCPLTHPLFLGTCQASDPQKALLNYEPVGGDIYSEESVTGHHLKFTARDEPMITLPRQFASREWYVIKGGMDVSQ casc_ecoli CRISPR system Cascade subunit CasC casC cas4 cse4 ygcJ JW2728 b2758 protein protein ecoli Escherichia coli (strain K12) MSNFINIHVLISHSPSCLNRDDMNMQKDAIFGGKRRVRISSQSLKRAMRKSGYYAQNIGESSLRTIHLAQLRDVLRQKLGERFDQKIIDKTLALLSGKSVDEAEKISADAVTPWVVGEIAWFCEQVAKAEADNLDDKKLLKVLKEDIAAIRVNLQQGVDIALSGRMATSGMMTELGKVDGAMSIAHAITTHQVDSDIDWFTAVDDLQEQGSAHLGTQEFSSGVFYRYANINLAQLQENLGGASREQALEIATHVVHMLATEVPGAKQRTYAAFNPADMVMVNFSDMPLSMANAFEKAVKAKDGFLQPSIQAFNQYWDRVANGYGLNGAAAQFSLSDVDPITAQVKQMPTLEQLKSWVRNNGEA cse2_ecoli CRISPR system Cascade subunit CasB casB cse2 ygcK JW2729 b2759 protein protein ecoli Escherichia coli (strain K12) MADEIDAMALYRAWQQLDNGSCAQIRRVSEPDELRDIPAFYRLVQPFGWENPRHQQALLRMVFCLSAGKNVIRHQDKKSEQTTGISLGRALANSGRINERRIFQLIRADRTADMVQLRRLLTHAEPVLDWPLMARMLTWWGKRERQQLLEDFVLTTNKNA cse1_ecoli CRISPR system Cascade subunit CasA casA cse1 ygcL JW2730 b2760 CRISPR type I-E/Ecoli-associated protein CasA/Cse1 CRISPR-associated protein CasA/Cse1 protein protein ecoli Escherichia coli (strain K12) MNLLIDNWIPVRPRNGGKVQIINLQSLYCSRDQWRLSLPRDDMELAALALLVCIGQIIAPAKDDVEFRHRIMNPLTEDEFQQLIAPWIDMFYLNHAEHPFMQTKGVKANDVTPMEKLLAGVSGATNCAFVNQPGQGEALCGGCTAIALFNQANQAPGFGGGFKSGLRGGTPVTTFVRGIDLRSTVLLNVLTLPRLQKQFPNESHTENQPTWIKPIKSNESIPASSIGFVRGLFWQPAHIELCDPIGIGKCSCCGQESNLRYTGFLKEKFTFTVNGLWPHPHSPCLVTVKKGEVEEKFLAFTTSAPSWTQISRVVVDKIIQNENGNRVAAVVNQFRNIAPQSPLELIMGGYRNNQASILERRHDVLMFNQGWQQYGNVINEIVTVGLGYKTALRKALYTFAEGFKNKDFKGAGVSVHETAERHFYRQSELLIPDVLANVNFSQADEVIADLRDKLHQLCEMLFNQSVAPYAHHPKLISTLALARATLYKHLRELKPQGGPSNG casa-casb-7 3268668 N/A 3268671 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3268672 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3268673 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3268674 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3268675 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false casb-casa-3 3268669 N/A 3268671 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3268672 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3268673 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3268674 unspecified role unspecified role bait bait StrepII-tag StrepII-tag strep ii tag strep ii tag Strep (II) Strep II Strep (II) Strep II n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3268675 unspecified role unspecified role prey prey ecolx Escherichia coli association association false false false casa-casb-8 3268670 N/A 3268671 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3268672 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3268673 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3268674 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3268675 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false