DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ larsen-2014-1 human Homo sapiens Human tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. DIP original experiment: DIP-262368X Nature cell biology 2014 IMEx The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. Only protein-protein interactions imex curation larsen-2014-2 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. DIP original experiment: DIP-262377X Nature cell biology 2014 IMEx The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. Only protein-protein interactions imex curation larsen-2014-3 human Homo sapiens Human tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. DIP original experiment: DIP-262367X Nature cell biology 2014 IMEx The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. Only protein-protein interactions imex curation larsen-2014-4 human Homo sapiens Human pull down pull down western blot western blot Immuno blot Immuno blot Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. DIP original experiment: DIP-262371X Nature cell biology 2014 IMEx The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. Only protein-protein interactions imex curation larsen-2014-5 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. DIP original experiment: DIP-262375X Nature cell biology 2014 IMEx The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. Only protein-protein interactions imex curation larsen-2014-6 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. DIP original experiment: DIP-262376X Nature cell biology 2014 IMEx The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. Only protein-protein interactions imex curation larsen-2014-7 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. DIP original experiment: DIP-262372X Nature cell biology 2014 IMEx The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. Only protein-protein interactions imex curation larsen-2014-8 human Homo sapiens Human tap tandem affinity purification TAP TAP tap ms participant Identification by mass spectrometry ms participant Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. DIP original experiment: DIP-262369X Nature cell biology 2014 IMEx The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. Only protein-protein interactions imex curation larsen-2014-9 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. DIP original experiment: DIP-262370X Nature cell biology 2014 IMEx The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. Only protein-protein interactions imex curation larsen-2014-10 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. DIP original experiment: DIP-262373X Nature cell biology 2014 IMEx The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. Only protein-protein interactions imex curation larsen-2014-11 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. DIP original experiment: DIP-262374X Nature cell biology 2014 IMEx The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage. Larsen DH., Hari F., Clapperton JA., Gwerder M., Gutsche K., Altmeyer M., Jungmichel S., Toledo LI., Fink D., Rask MB., Grøfte M., Lukas C., Nielsen ML., Smerdon SJ., Lukas J., Stucki M. Only protein-protein interactions imex curation nbn_human Nibrin Nijmegen breakage syndrome protein 1 Cell cycle regulatory protein p95 NBN NBS1 NBS P95 protein protein human Homo sapiens Human MWKLLPAAGPAGGEPYRLLTGVEYVVGRKNCAILIENDQSISRNHAVLTANFSVTNLSQTDEIPVLTLKDNSKYGTFVNEEKMQNGFSRTLKSGDGITFGVFGSKFRIEYEPLVACSSCLDVSGKTALNQAILQLGGFTVNNWTEECTHLVMVSVKVTIKTICALICGRPIVKPEYFTEFLKAVESKKQPPQIESFYPPLDEPSIGSKNVDLSGRQERKQIFKGKTFIFLNAKQHKKLSSAVVFGGGEARLITEENEEEHNFFLAPGTCVVDTGITNSQTLIPDCQKKWIQSIMDMLQRQGLRPIPEAEIGLAVIFMTTKNYCDPQGHPSTGLKTTTPGPSLSQGVSVDEKLMPSAPVNTTTYVADTESEQADTWDLSERPKEIKVSKMEQKFRMLSQDAPTVKESCKTSSNNNSMVSNTLAKMRIPNYQLSPTKLPSINKSKDRASQQQQTNSIRNYFQPSTKKRERDEENQEMSSCKSARIETSCSLLEQTQPATPSLWKNKEQHLSENEPVDTNSDNNLFTDTDLKSIVKNSASKSHAAEKLRSNKKREMDDVAIEDEVLEQLFKDTKPELEIDVKVQKQEEDVNVRKRPRMDIETNDTFSDEAVPESSKISQENEIGKKRELKEDSLWSAKEISNNDKLQDDSEMLPKKLLLTEFRSLVIKNSTSRNPSGINDDYGQLKNFKKFKKVTYPGAGKLPHIIGGSDLIAHHARKNTELEEWLRQEMEVQNQHAKEESLADDLFRYNPYLKRRR tcof_human Treacle protein TCOF1 Treacher Collins syndrome protein protein protein human Homo sapiens Human MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV mdc1_human Mediator of DNA damage checkpoint protein 1 Nuclear factor with BRCT domains 1 MDC1 KIAA0170 NFBD1 protein protein human Homo sapiens Human MEDTQAIDWDVEEEEETEQSSESLRCNVEPVGRLHIFSGAHGPEKDFPLHLGKNVVGRMPDCSVALPFPSISKQHAEIEILAWDKAPILRDCGSLNGTQILRPPKVLSPGVSHRLRDQELILFADLLCQYHRLDVSLPFVSRGPLTVEETPRVQGETQPQRLLLAEDSEEEVDFLSERRMVKKSRTTSSSVIVPESDEEGHSPVLGGLGPPFAFNLNSDTDVEEGQQPATEEASSAARRGATVEAKQSEAEVVTEIQLEKDQPLVKERDNDTKVKRGAGNGVVPAGVILERSQPPGEDSDTDVDDDSRPPGRPAEVHLERAQPFGFIDSDTDAEEERIPATPVVIPMKKRKIFHGVGTRGPGAPGLAHLQESQAGSDTDVEEGKAPQAVPLEKSQASMVINSDTDDEEEVSAALTLAHLKESQPAIWNRDAEEDMPQRVVLLQRSQTTTERDSDTDVEEEELPVENREAVLKDHTKIRALVRAHSEKDQPPFGDSDDSVEADKSSPGIHLERSQASTTVDINTQVEKEVPPGSAIIHIKKHQVSVEGTNQTDVKAVGGPAKLLVVSLEEAWPLHGDCETDAEEGTSLTASVVADVRKSQLPAEGDAGAEWAAAVLKQERAHEVGAQGGPPVAQVEQDLPISRENLTDLVVDTDTLGESTQPQREGAQVPTGREREQHVGGTKDSEDNYGDSEDLDLQATQCFLENQGLEAVQSMEDEPTQAFMLTPPQELGPSHCSFQTTGTLDEPWEVLATQPFCLRESEDSETQPFDTHLEAYGPCLSPPRAIPGDQHPESPVHTEPMGIQGRGRQTVDKVMGIPKETAERVGPERGPLERETEKLLPERQTDVTGEEELTKGKQDREQKQLLARDTQRQESDKNGESASPERDRESLKVEIETSEEIQEKQVQKQTLPSKAFEREVERPVANRECDPAELEEKVPKVILERDTQRGEPEGGSQDQKGQASSPTPEPGVGAGDLPGPTSAPVPSGSQSGGRGSPVSPRRHQKGLLNCKMPPAEKASRIRAAEKVSRGDQESPDACLPPTVPEAPAPPQKPLNSQSQKHLAPPPLLSPLLPSIKPTVRKTRQDGSQEAPEAPLSSELEPFHPKPKIRTRKSSRMTPFPATSAAPEPHPSTSTAQPVTPKPTSQATRSRTNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTSQVTRGRKSRSSVKTPETVVPTALELQPSTSTDRPVTSEPTSQATRGRKNRSSVKTPEPVVPTAPELQPSTSTDQPVTSEPTYQATRGRKNRSSVKTPEPVVPTAPELRPSTSTDRPVTPKPTSRTTRSRTNMSSVKTPETVVPTAPELQISTSTDQPVTPKPTSRTTRSRTNMSSVKNPESTVPIAPELPPSTSTEQPVTPEPTSRATRGRKNRSSGKTPETLVPTAPKLEPSTSTDQPVTPEPTSQATRGRTNRSSVKTPETVVPTAPELQPSTSTDQPVTPEPTSQATRGRTDRSSVKTPETVVPTAPELQASASTDQPVTSEPTSRTTRGRKNRSSVKTPETVVPAAPELQPSTSTDQPVTPEPTSRATRGRTNRSSVKTPESIVPIAPELQPSTSRNQLVTPEPTSRATRCRTNRSSVKTPEPVVPTAPEPHPTTSTDQPVTPKLTSRATRRKTNRSSVKTPKPVEPAASDLEPFTPTDQSVTPEAIAQGGQSKTLRSSTVRAMPVPTTPEFQSPVTTDQPISPEPITQPSCIKRQRAAGNPGSLAAPIDHKPCSAPLEPKSQASRNQRWGAVRAAESLTAIPEPASPQLLETPIHASQIQKVEPAGRSRFTPELQPKASQSRKRSLATMDSPPHQKQPQRGEVSQKTVIIKEEEEDTAEKPGKEEDVVTPKPGKRKRDQAEEEPNRIPSRSLRRTKLNQESTAPKVLFTGVVDARGERAVLALGGSLAGSAAEASHLVTDRIRRTVKFLCALGRGIPILSLDWLHQSRKAGFFLPPDEYVVTDPEQEKNFGFSLQDALSRARERRLLEGYEIYVTPGVQPPPPQMGEIISCCGGTYLPSMPRSYKPQRVVITCPQDFPHCSIPLRVGLPLLSPEFLLTGVLKQEAKPEAFVLSPLEMSST rpa34_human DNA-directed RNA polymerase I subunit RPA34 RNA polymerase I-associated factor PAF49 POLR1G A34.5 ASE1 Antisense to ERCC-1 protein CAST CD3-epsilon-associated protein CD3EAP DNA-directed RNA polymerase I subunit G PAF49 protein protein human Homo sapiens Human MEEPQAGDAARFSCPPNFTAKPPASESPRFSLEALTGPDTELWLIQAPADFAPECFNGRHVPLSGSQIVKGKLAGKRHRYRVLSSCPQAGEATLLAPSTEAGGGLTCASAPQGTLRILEGPQQSLSGSPLQPIPASPPPQIPPGLRPRFCAFGGNPPVTGPRSALAPNLLTSGKKKKEMQVTEAPVTQEAVNGHGALEVDMALGSPEMDVRKKKKKKNQQLKEPEAAGPVGTEPTVETLEPLGVLFPSTTKKRKKPKGKETFEPEDKTVKQEQINTEPLEDTVLSPTKKRKRQKGTEGMEPEEGVTVESQPQVKVEPLEEAIPLPPTKKRKKEKGQMAMMEPGTEAMEPVEPEMKPLESPGGTMAPQQPEGAKPQAQAALAAPKKKTKKEKQQDATVEPETEVVGPELPDDLEPQAAPTSTKKKKKKKERGHTVTEPIQPLEPELPGEGQPEARATPGSTKKRKKQSQESRMPETVPQEEMPGPPLNSESGEEAPTGRDKKRKQQQQQPV rpa2_human DNA-directed RNA polymerase I subunit RPA2 POLR1B DNA-directed RNA polymerase I 135 kDa polypeptide protein protein human Homo sapiens Human MDPGSRWRNLPSGPSLKHLTDPSYGIPREQQKAALQELTRAHVESFNYAVHEGLGLAVQAIPPFEFAFKDERISFTILDAVISPPTVPKGTICKEANVYPAECRGRRSTYRGKLTADINWAVNGISKGIIKQFLGYVPIMVKSKLCNLRNLPPQALIEHHEEAEEMGGYFIINGIEKVIRMLIMPRRNFPIAMIRPKWKTRGPGYTQYGVSMHCVREEHSAVNMNLHYLENGTVMLNFIYRKELFFLPLGFALKALVSFSDYQIFQELIKGKEDDSFLRNSVSQMLRIVMEEGCSTQKQVLNYLGECFRVKLNVPDWYPNEQAAEFLFNQCICIHLKSNTEKFYMLCLMTRKLFALAKGECMEDNPDSLVNQEVLTPGQLFLMFLKEKLEGWLVSIKIAFDKKAQKTSVSMNTDNLMRIFTMGIDLTKPFEYLFATGNLRSKTGLGLLQDSGLCVVADKLNFIRYLSHFRCVHRGADFAKMRTTTVRRLLPESWGFLCPVHTPDGEPCGLMNHLTAVCEVVTQFVYTASIPALLCNLGVTPIDGAPHRSYSECYPVLLDGVMVGWVDKDLAPGIADSLRHFKVLREKRIPPWMEVVLIPMTGKPSLYPGLFLFTTPCRLVRPVQNLALGKEELIGTMEQIFMNVAIFEDEVFAGVTTHQELFPHSLLSVIANFIPFSDHNQSPRNMYQCQMGKQTMGFPLLTYQDRSDNKLYRLQTPQSPLVRPSMYDYYDMDNYPIGTNAIVAVISYTGYDMEDAMIVNKASWERGFAHGSVYKSEFIDLSEKIKQGDSSLVFGIKPGDPRVLQKLDDDGLPFIGAKLQYGDPYYSYLNLNTGESFVMYYKSKENCVVDNIKVCSNDTGSGKFKCVCITMRVPRNPTIGDKFASRHGQKGILSRLWPAEDMPFTESGMVPDILFNPHGFPSRMTIGMLIESMAGKSAALHGLCHDATPFIFSEENSALEYFGEMLKAAGYNFYGTERLYSGISGLELEADIFIGVVYYQRLRHMVSDKFQVRTTGARDRVTNQPIGGRNVQGGIRFGEMERDALLAHGTSFLLHDRLFNCSDRSVAHVCVKCGSLLSPLLEKPPPSWSAMRNRKYNCTLCSRSDTIDTVSVPYVFRYFVAELAAMNIKVKLDVV rpa1_human DNA-directed RNA polymerase I subunit RPA1 POLR1A DNA-directed RNA polymerase I subunit A DNA-directed RNA polymerase I largest subunit RNA polymerase I 194 kDa subunit A190 protein protein human Homo sapiens Human MLISKNMPWRRLQGISFGMYSAEELKKLSVKSITNPRYLDSLGNPSANGLYDLALGPADSKEVCSTCVQDFSNCSGHLGHIELPLTVYNPLLFDKLYLLLRGSCLNCHMLTCPRAVIHLLLCQLRVLEVGALQAVYELERILNRFLEENPDPSASEIREELEQYTTEIVQNNLLGSQGAHVKNVCESKSKLIALFWKAHMNAKRCPHCKTGRSVVRKEHNSKLTITFPAMVHRTAGQKDSEPLGIEEAQIGKRGYLTPTSAREHLSALWKNEGFFLNYLFSGMDDDGMESRFNPSVFFLDFLVVPPSRYRPVSRLGDQMFTNGQTVNLQAVMKDVVLIRKLLALMAQEQKLPEEVATPTTDEEKDSLIAIDRSFLSTLPGQSLIDKLYNIWIRLQSHVNIVFDSEMDKLMMDKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTALSAVDMTQREAVAKQLLTPATGAPKPQGTKIVCRHVKNGDILLLNRQPTLHRPSIQAHRARILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGASMTTRGCFFTREHYMELVYRGLTDKVGRVKLLSPSILKPFPLWTGKQVVSTLLINIIPEDHIPLNLSGKAKITGKAWVKETPRSVPGFNPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETSGKVLTCLARLFTAYLQLYRGFTLGVEDILVKPKADVKRQRIIEESTHCGPQAVRAALNLPEAASYDEVRGKWQDAHLGKDQRDFNMIDLKFKEEVNHYSNEINKACMPFGLHRQFPENSLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQRCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLASNYEVIMKSQHLHEVLSRADPKKALHHFRAIKKWQSKHPNTLLRRGAFLSYSQKIQEAVKALKLESENRNGRSPGTQEMLRMWYELDEESRRKYQKKAAACPDPSLSVWRPDIYFASVSETFETKVDDYSQEWAAQTEKSYEKSELSLDRLRTLLQLKWQRSLCEPGEAVGLLAAQSIGEPSTQMTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKALKRVKSLKKQLTRVCLGEVLQKIDVQESFCMEEKQNKFQVYQLRFQFLPHAYYQQEKCLRPEDILRFMETRFFKLLMESIKKKNNKASAFRNVNTRRATQRDLDNAGELGRSRGEQEGDEEEEGHIVDAEAEEGDADASDAKRKEKQEEEVDYESEEEEEREGEENDDEDMQEERNPHREGARKTQEQDEEVGLGTEEDPSLPALLTQPRKPTHSQEPQGPEAMERRVQAVREIHPFIDDYQYDTEESLWCQVTVKLPLMKINFDMSSLVVSLAHGAVIYATKGITRCLLNETTNNKNEKELVLNTEGINLPELFKYAEVLDLRRLYSNDIHAIANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNRFGIRSNSSPLQQMTFETSFQFLKQATMLGSHDELRSPSACLVVGKVVRGGTGLFELKQPLR nbn-tcof1-1 3268395 N/A 3268406 unspecified role unspecified role bait bait TAP-tag TAP-tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false O60934:p.Arg28Ala R28A mutation mutation certain certain sequence position certain certain certain sequence position certain false Region 1-382 Region 1-382 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3268407 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false nbn-tcof1-2 3268396 N/A 3268406 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false O60934:p.[Arg28Ala;Lys160Met] R28A/K160M mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Region 1-382 Region 1-382 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3268407 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false nbn-cd3eap-1 3268397 N/A 3268408 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3268406 unspecified role unspecified role bait bait TAP-tag TAP-tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region 1-382 Region 1-382 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3268409 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3268410 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3268411 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3268407 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false tcof1-nbn-1 3268398 N/A 3268406 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3268407 unspecified role unspecified role bait bait Strep-tag Strep-tag strep tag strep tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false nbn-tcof1-3 3268399 N/A 3268406 unspecified role unspecified role unspecified role unspecified role Region 1-382 Region 1-382 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3268407 unspecified role unspecified role unspecified role unspecified role Region 204-213 Region 204-213 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false pS207pT210 pS207pT210 phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false nbn-tcof1-4 3268400 N/A 3268406 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-382 Region 1-382 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3268407 unspecified role unspecified role prey prey Q13428:p.Thr210Ala T210A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false tcof1-nbn-2 3268401 N/A 3268406 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3268407 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false nbn-cd3eap-2 3268402 N/A 3268408 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3268406 unspecified role unspecified role bait bait TAP-tag TAP-tag tandem tag tandem tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false O60934:p.Arg28Ala R28A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Region 1-382 Region 1-382 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3268409 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3268410 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3268411 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false nbn-tcof1-5 3268403 N/A 3268406 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-382 Region 1-382 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O60934:p.[Arg28Ala;Lys160Met] R28A/K160M mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3268407 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false nbn-tcof1-6 3268404 N/A 3268406 unspecified role unspecified role bait bait O60934:p.Arg28Ala R28A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O60934:p.[Arg28Ala;Lys160Met] R28A/K160M mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Region 1-382 Region 1-382 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Tag used for western blot detection. human Homo sapiens Human N/A 3268407 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false tcof1-nbn-3 3268405 N/A 3268406 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3268407 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-330 Region 1-330 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 1119-1481 Region 1119-1481 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false