DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ schfer-2014-1 trini Trichoplusia ni (Cabbage looper) Cabbage looper ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Schäfer IB., Rode M., Bonneau F., Schüssler S., Conti E. DIP original experiment: DIP-265620X Nature structural & molecular biology 2014 IMEx The structure of the Pan2-Pan3 core complex reveals cross-talk between deadenylase and pseudokinase. Schäfer IB., Rode M., Bonneau F., Schüssler S., Conti E. Only protein-protein interactions imex curation schfer-2014-2 trini Trichoplusia ni (Cabbage looper) Cabbage looper pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Schäfer IB., Rode M., Bonneau F., Schüssler S., Conti E. DIP original experiment: DIP-265622X Nature structural & molecular biology 2014 IMEx The structure of the Pan2-Pan3 core complex reveals cross-talk between deadenylase and pseudokinase. Schäfer IB., Rode M., Bonneau F., Schüssler S., Conti E. Only protein-protein interactions imex curation schfer-2014-3 trini Trichoplusia ni (Cabbage looper) Cabbage looper x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Schäfer IB., Rode M., Bonneau F., Schüssler S., Conti E. DIP original experiment: DIP-265618X Nature structural & molecular biology 2014 IMEx The structure of the Pan2-Pan3 core complex reveals cross-talk between deadenylase and pseudokinase. Schäfer IB., Rode M., Bonneau F., Schüssler S., Conti E. Only protein-protein interactions imex curation schfer-2014-4 trini Trichoplusia ni (Cabbage looper) Cabbage looper pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Schäfer IB., Rode M., Bonneau F., Schüssler S., Conti E. DIP original experiment: DIP-265621X Nature structural & molecular biology 2014 IMEx The structure of the Pan2-Pan3 core complex reveals cross-talk between deadenylase and pseudokinase. Schäfer IB., Rode M., Bonneau F., Schüssler S., Conti E. Only protein-protein interactions imex curation schfer-2014-5 trini Trichoplusia ni (Cabbage looper) Cabbage looper molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Schäfer IB., Rode M., Bonneau F., Schüssler S., Conti E. DIP original experiment: DIP-265619X Nature structural & molecular biology 2014 IMEx The structure of the Pan2-Pan3 core complex reveals cross-talk between deadenylase and pseudokinase. Schäfer IB., Rode M., Bonneau F., Schüssler S., Conti E. Only protein-protein interactions imex curation pan3_yeast PAN2-PAN3 deadenylation complex subunit PAN3 PAN3 ECM35 PAB1P-dependent poly(A)-specific ribonuclease Poly(A)-nuclease deadenylation complex subunit 3 YKL025C protein protein yeast Saccharomyces cerevisiae Baker's yeast MDKINPDWAKDIPCRNITIYGYCKKEKEGCPFKHSDNTTATTINDVPPPIDVGEATTPTMTSVPKFNAKVSASFTPMTVGSDSLTTVTNTTSAATNATGNIAMAATSATASTVNPMINPIVNSSLVNNNNNNSNISISIPTTASSSNYDPFNAPIFTPSSTSSIHTNANAHSFPFPSIANSGGININATDDNSNNMSMANNVPPPMQPPPIESSNLKYPRIYPPPHSLLQYHLYAPEQPSSLKSLLKPNERSADQLFIPNNIREDLTKKNLSILQVFPSSGKVIPSIVQDYFNLVPLNFNNNDFLNKTTLFKVFSNYDGKAYVLKRLPNIDKSMNPNKISKIYQIWSKINCTNLIKFRDIFQTTKFGDLSICLVFDYYPNSLSLYDYHFVNFPKFPITNNYLWIYLVQLTNVINSIHSQNLSIGNTLNWRKVLITGDPGRIKLSHCNFMDLLFNDDTDTVVSSGGSTIEGQQQLDYKYLGELLFNLSINIENSNNNTAPKEYRLEEITPQSIDDMRQIDDKFKDVLKYLISDNGDSKKSIHDLTSHFYDKMFMVLESSQTYTEYMESVLSRELENGRLFRLVNKLNCIFGRIESRIDINWSESGTKFPIILFYDYVFHQVDSNGKPIMDLTHVLRCLNKLDAGIQEKLMLVTPDELNCIIISYKELKDLIESTFRSITQ DE03E15BF4A5DA7C pan2_yeast PAN2-PAN3 deadenylation complex catalytic subunit PAN2 PAN2 PAB1P-dependent poly(A)-specific ribonuclease Poly(A)-nuclease deadenylation complex subunit 2 YGL094C protein protein yeast Saccharomyces cerevisiae Baker's yeast MNNWQHFFNNPVDLSEHLKKPYFRFDNRDKEITAISFDEKANLIWSGDSYGCISSYDPTFQLYTRYRGHIGGNSVKDILSHRDGILSISEDSLHFANRRGVTKLNLTSIDIAAFSELNTMCYSPHSLKNNIYCGGDNTNWGIASIDLNRGCLDSLLNYSSKVKLMCSNNKVLSIGRQTGTVDLLDPTSNRTIKSFNAHSASISAMDLRDNTLVTVGKSKRFYNLYADPFVNVYDLRTMRQLPPVSFSKGTTMGSGGADFVQLHPLLPTVMIVASSSGSFDFIDLSNPTLRTQYVHPCQSIKKLCLSPNGDVLGILEADNHLDTWRRSSNNMGMFTNTPEMLAYPDYFNDITSDGPISVDDETYPLSSVGMPYYLDKLLSAWPPVVFKSEGTIPQLTGKSPLPSSGKLKSNLAVISSQNEKLSTQEFPLLRYDRTKYGMRNAIPDYVCLRDIRKQITSGLETSDIQTYTSINKYEVPPAYSRLPLTSGRFGTDNFDFTPFNNTEYSGLDPDVDNHYTNAIIQLYRFIPEMFNFVVGCLKDENFETTLLTDLGYLFDMMERSHGKICSSSNFQASLKSLTDKRQLENGEPQEHLEEYLESLCIRESIEDFNSSESIKRNMPQKFNRFLLSQLIKEEAQTVNHNITLNQCFGLETEIRTECSCDHYDTTVKLLPSLSISGINKTVIKQLNKKSNGQNILPYIEYAMKNVTQKNSICPTCGKTETITQECTVKNLPSVLSLELSLLDTEFSNIRSSKNWLTSEFYGSIIKNKAVLRSTASELKGTSHIFKYELNGYVAKITDNNNETRLVTYVKKYNPKENCFKWLMFNDYLVVEITEEEALKMTYPWKTPEIIIYCDAEELRKPFFSVDTYSINYDILFRDYFANGIRDTARREYKLLTHDEAPKSGTLVAIDAEFVSLQSELCEIDHQGIRSIIRPKRTALARISIIRGEEGELYGVPFVDDYVVNTNHIEDYLTRYSGILPGDLDPEKSTKRLVRRNVVYRKVWLLMQLGCVFVGHGLNNDFKHININVPRNQIRDTAIYFLQGKRYLSLRYLAYVLLGMNIQEGNHDSIEDAHTALILYKKYLHLKEKAIFEKVLNSVYEEGRAHNFKVPETSKG 4F91B737F761AE5A pan2-pan3-13 3266676 N/A 3266681 unspecified role unspecified role unspecified role unspecified role Region 226-679 Region 226-679 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3266682 unspecified role unspecified role unspecified role unspecified role Region 340-1115 Region 340-1115 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false pan3-pan2-3 3266677 N/A 3266681 unspecified role unspecified role bait bait Region 226-679 Region 226-679 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3266682 unspecified role unspecified role prey prey Region 340-1115 Region 340-1115 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false pan2-pan3-14 3266678 N/A 3266681 unspecified role unspecified role unspecified role unspecified role Region 226-679 Region 226-679 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3266682 unspecified role unspecified role unspecified role unspecified role Region 340-1115 Region 340-1115 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false pan2-pan3-15 3266679 N/A 3266681 unspecified role unspecified role prey prey Region 226-679 Region 226-679 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3266682 unspecified role unspecified role bait bait Region 340-1115 Region 340-1115 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false pan2-pan3-16 3266680 N/A 3266681 unspecified role unspecified role unspecified role unspecified role Region 226-679 Region 226-679 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3266682 unspecified role unspecified role unspecified role unspecified role Region 340-1115 Region 340-1115 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false