DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ burke-2014-1 in vitro In vitro h1-h2 ms mass spectrometry study of hydrogen/deuterium exchange h1-h2 ms predetermined predetermined participant predetermined Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. DIP original experiment: DIP-266372X Science (New York, N.Y.) 2014 IMEx Structures of PI4KIIIβ complexes show simultaneous recruitment of Rab11 and its effectors. Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. Only protein-protein interactions imex curation burke-2014-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. DIP original experiment: DIP-266368X Science (New York, N.Y.) 2014 IMEx Structures of PI4KIIIβ complexes show simultaneous recruitment of Rab11 and its effectors. Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. Only protein-protein interactions imex curation burke-2014-3 in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. DIP original experiment: DIP-266376X Science (New York, N.Y.) 2014 IMEx Structures of PI4KIIIβ complexes show simultaneous recruitment of Rab11 and its effectors. Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. Only protein-protein interactions imex curation burke-2014-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. DIP original experiment: DIP-266370X Science (New York, N.Y.) 2014 IMEx Structures of PI4KIIIβ complexes show simultaneous recruitment of Rab11 and its effectors. Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. Only protein-protein interactions imex curation burke-2014-5 in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. DIP original experiment: DIP-266374X Science (New York, N.Y.) 2014 IMEx Structures of PI4KIIIβ complexes show simultaneous recruitment of Rab11 and its effectors. Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. Only protein-protein interactions imex curation burke-2014-6 in vitro In vitro gdp_gtp exchange gdp/gtp exchange assay gtp/gdp exchange guanine nucleotide exchange assay gtp/gdp exchange guanine nucleotide exchange assay gdp_gtp exchange predetermined predetermined participant predetermined Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. DIP original experiment: DIP-266371X Science (New York, N.Y.) 2014 IMEx Structures of PI4KIIIβ complexes show simultaneous recruitment of Rab11 and its effectors. Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. Only protein-protein interactions imex curation burke-2014-7 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. DIP original experiment: DIP-266373X Science (New York, N.Y.) 2014 IMEx Structures of PI4KIIIβ complexes show simultaneous recruitment of Rab11 and its effectors. Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. Only protein-protein interactions imex curation burke-2014-8 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. DIP original experiment: DIP-266369X Science (New York, N.Y.) 2014 IMEx Structures of PI4KIIIβ complexes show simultaneous recruitment of Rab11 and its effectors. Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. Only protein-protein interactions imex curation burke-2014-9 in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. DIP original experiment: DIP-266375X Science (New York, N.Y.) 2014 IMEx Structures of PI4KIIIβ complexes show simultaneous recruitment of Rab11 and its effectors. Burke JE., Inglis AJ., Perisic O., Masson GR., McLaughlin SH., Rutaganira F., Shokat KM., Williams RL. Only protein-protein interactions imex curation rb11a_human Ras-related protein Rab-11A YL8 RAB11A RAB11 protein protein human Homo sapiens Human MGTRDDEYDYLFKVVLIGDSGVGKSNLLSRFTRNEFNLESKSTIGVEFATRSIQVDGKTIKAQIWDTAGQERYRAITSAYYRGAVGALLVYDIAKHLTYENVERWLKELRDHADSNIVIMLVGNKSDLRHLRAVPTDEARAFAEKNGLSFIETSALDSTNVEAAFQTILTEIYRIVSQKQMSDRRENDMSPSNNVVPIHVPPTTENKPKVQCCQNI q9ubf8-2 Phosphatidylinositol 4-kinase beta PI4KIII PI4KB PIK4CB NPIK-C NPIK PI4K92 protein protein human Homo sapiens Human MGDTVVEPAPLKPTSEPTSGPPGNNGGSLLSVITEGVGELSVIDPEVAQKACQEVLEKVKLLHGGVAVSSRGTPLELVNGDGVDSEIRCLDDPPAQIREEEDEMGAAVASGTAKGARRRRQNNSAKQSWLLRLFESKLFDISMAISYLYNSKEPGVQAYIGNRLFCFRNEDVDFYLPQLLNMYIHMDEDVGDAIKPYIVHRCRQSINFSLQCALLLGAYSSDMHISTQRHSRGTKLRKLILSDELKPAHRKRELPSLSPAPDTGLSPSKRTHQRSKSDATASISLSSNLKRTASNPKVENEDEPVRLAPEREFIKSLMAIGKRLATLPTKEQKTQRLISELSLLNHKLPARVWLPTAGFDHHVVRVPHTQAVVLNSKDKAPYLIYVEVLECENFDTTSVPARIPENRIRSTRSVENLPECGITHEQRAGSFSTVPNYDNDDEAWSVDDIGELQVELPEVHTNSCDNISQFSVDSITSQESKEPVFIAAGDIRRRLSEQLAHTPTAFKRDPEDPSAVALKEPWQEKVRRIREGSPYGHLPNWRLLSVIVKCGDDLRQELLAFQVLKQLQSIWEQERVPLWIKPYKILVISADSGMIEPVVNAVSIHQVKKQSQLSLLDYFLQEHGSYTTEAFLSAQRNFVQSCAGYCLVCYLLQVKDRHNGNILLDAEGHIIHIDFGFILSSSPRNLGFETSAFKLTTEFVDVMGGLDGDMFNYYKMLMLQGLIAARKHMDKVVQIVEIMQQGSQLPCFHGSSTIRNLKERFHMSMTEEQLQLLVEQMVDGSMRSITTKLYDGFQYLTNGIM Contains a phosphoserine at position 294. o75154-1 Rab11 family-interacting protein 3 RAB11FIP3 ARFO1 KIAA0665 Arfophilin-1 EF hands-containing Rab-interacting protein MU-MB-17.148 protein protein human Homo sapiens Human MASAPPASPPGSEPPGPDPEPGGPDGPGAAQLAPGPAELRLGAPVGGPDPQSPGLDEPAPGAAADGGARWSAGPAPGLEGGPRDPGPSAPPPRSGPRGQLASPDAPGPGPRSEAPLPELDPLFSWTEEPEECGPASCPESAPFRLQGSSSSHRARGEVDVFSPFPAPTAGELALEQGPGSPPQPSDLSQTHPLPSEPVGSQEDGPRLRAVFDALDGDGDGFVRIEDFIQFATVYGAEQVKDLTKYLDPSGLGVISFEDFYQGITAIRNGDPDGQCYGGVASAQDEEPLACPDEFDDFVTYEANEVTDSAYMGSESTYSECETFTDEDTSTLVHPELQPEGDADSAGGSAVPSECLDAMEEPDHGALLLLPGRPHPHGQSVITVIGGEEHFEDYGEGSEAELSPETLCNGQLGCSDPAFLTPSPTKRLSSKKVARYLHQSGALTMEALEDPSPELMEGPEEDIADKVVFLERRVLELEKDTAATGEQHSRLRQENLQLVHRANALEEQLKEQELRACEMVLEETRRQKELLCKMEREKSIEIENLQTRLQQLDEENSELRSCTPCLKANIERLEEEKQKLLDEIESLTLRLSEEQENKRRMGDRLSHERHQFQRDKEATQELIEDLRKQLEHLQLLKLEAEQRRGRSSSMGLQEYHSRARESELEQEVRRLKQDNRNLKEQNEELNGQIITLSIQGAKSLFSTAFSESLAAEISSVSRDELMEAIQKQEEINFRLQDYIDRIIVAIMETNPSILEVK pi4kb-rab11a-1 3266527 N/A 3266536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3266537 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false pi4kb-rab11a-2 3266528 N/A 3266538 unspecified role unspecified role unspecified role unspecified role Region 713-756 Region 713-756 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3266536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3266537 unspecified role unspecified role unspecified role unspecified role Region 121-799 Region 121-799 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false rab11fip3-rab11a-2 3266529 N/A 3266538 unspecified role unspecified role bait bait Region 713-756 Region 713-756 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3266536 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false pi4kb-rab11a-3 3266530 N/A 3266538 unspecified role unspecified role unspecified role unspecified role Region 713-756 Region 713-756 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3266536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3266537 unspecified role unspecified role unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm physical association physical association false false false rab11a-pi4kb-1 3266531 N/A 3266536 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3266537 unspecified role unspecified role prey prey Q9UBF8-2:p.Tyr159Ala Y159A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9UBF8-2:p.Asn162Ala N162A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9UBF8-2:p.[Tyr159Ala;Asn162Ala] Y159A/N162A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false Q9UBF8-2:p.Gly155Asp G155D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9UBF8-2:p.Phe165Ala F165A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false pi4kb-rab11a-4 3266532 N/A 3266536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3266537 unspecified role unspecified role unspecified role unspecified role Q9UBF8-2:p.[Tyr159Ala;Asn162Ala] Y159A/N162A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false rab11a-pi4kb-2 3266533 N/A 3266536 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3266537 unspecified role unspecified role prey prey Q9UBF8-2:p.Tyr159Ala Y159A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9UBF8-2:p.Asn162Ala N162A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false pi4kb-rab11a-5 3266534 N/A 3266536 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3266537 unspecified role unspecified role unspecified role unspecified role Region 121-799 Region 121-799 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false rab11fip3-pi4kb-1 3266535 N/A 3266538 unspecified role unspecified role bait bait Region 713-756 Region 713-756 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3266536 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3266537 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm association association false false false