DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ grishin-2014-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Grishin AM., Condos TE., Barber KR., Campbell-Valois FX., Parsot C., Shaw GS., Cygler M. DIP original experiment: DIP-261922X Structure (London, England : 1993) 2014 IMEx Structural basis for the inhibition of host protein ubiquitination by Shigella effector kinase OspG. Grishin AM., Condos TE., Barber KR., Campbell-Valois FX., Parsot C., Shaw GS., Cygler M. Only protein-protein interactions imex curation grishin-2014-4 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Grishin AM., Condos TE., Barber KR., Campbell-Valois FX., Parsot C., Shaw GS., Cygler M. DIP original experiment: DIP-261920X Structure (London, England : 1993) 2014 IMEx Structural basis for the inhibition of host protein ubiquitination by Shigella effector kinase OspG. Grishin AM., Condos TE., Barber KR., Campbell-Valois FX., Parsot C., Shaw GS., Cygler M. Only protein-protein interactions imex curation grishin-2014-5 in vitro In vitro comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Grishin AM., Condos TE., Barber KR., Campbell-Valois FX., Parsot C., Shaw GS., Cygler M. DIP original experiment: DIP-261926X Structure (London, England : 1993) 2014 IMEx Structural basis for the inhibition of host protein ubiquitination by Shigella effector kinase OspG. Grishin AM., Condos TE., Barber KR., Campbell-Valois FX., Parsot C., Shaw GS., Cygler M. Only protein-protein interactions imex curation grishin-2014-6 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Grishin AM., Condos TE., Barber KR., Campbell-Valois FX., Parsot C., Shaw GS., Cygler M. DIP original experiment: DIP-261924X Structure (London, England : 1993) 2014 IMEx Structural basis for the inhibition of host protein ubiquitination by Shigella effector kinase OspG. Grishin AM., Condos TE., Barber KR., Campbell-Valois FX., Parsot C., Shaw GS., Cygler M. Only protein-protein interactions imex curation grishin-2014-7 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Grishin AM., Condos TE., Barber KR., Campbell-Valois FX., Parsot C., Shaw GS., Cygler M. DIP original experiment: DIP-261925X Structure (London, England : 1993) 2014 IMEx Structural basis for the inhibition of host protein ubiquitination by Shigella effector kinase OspG. Grishin AM., Condos TE., Barber KR., Campbell-Valois FX., Parsot C., Shaw GS., Cygler M. Only protein-protein interactions imex curation grishin-2014-8 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Grishin AM., Condos TE., Barber KR., Campbell-Valois FX., Parsot C., Shaw GS., Cygler M. DIP original experiment: DIP-261923X Structure (London, England : 1993) 2014 IMEx Structural basis for the inhibition of host protein ubiquitination by Shigella effector kinase OspG. Grishin AM., Condos TE., Barber KR., Campbell-Valois FX., Parsot C., Shaw GS., Cygler M. Only protein-protein interactions imex curation grishin-2014-9 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Grishin AM., Condos TE., Barber KR., Campbell-Valois FX., Parsot C., Shaw GS., Cygler M. DIP original experiment: DIP-261921X Structure (London, England : 1993) 2014 IMEx Structural basis for the inhibition of host protein ubiquitination by Shigella effector kinase OspG. Grishin AM., Condos TE., Barber KR., Campbell-Valois FX., Parsot C., Shaw GS., Cygler M. Only protein-protein interactions imex curation ospg_shifl Protein kinase OspG ospG Effector protein OspG pWR501_0237 SFLP087 CP0227 protein protein shifl Shigella flexneri MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFEDVNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVPGTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRNIYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL p68036-1 Ubiquitin-conjugating enzyme E2 L3 E2 ubiquitin-conjugating enzyme L3 L-UBC UbcH7 Ubiquitin carrier protein L3 Ubiquitin-conjugating enzyme E2-F1 Ubiquitin-protein ligase L3 UBE2L3 UBCE7 UBCH7 protein protein human Homo sapiens Human MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG prkn_human E3 ubiquitin-protein ligase parkin PRKN PARK2 Parkin RBR E3 ubiquitin-protein ligase Parkinson juvenile disease protein 2 protein protein human Homo sapiens Human MIVFVRFNSSHGFPVEVDSDTSIFQLKEVVAKRQGVPADQLRVIFAGKELRNDWTVQNCDLDQQSIVHIVQRPWRKGQEMNATGGDDPRNAAGGCEREPQSLTRVDLSSSVLPGDSVGLAVILHTDSRKDSPPAGSPAGRSIYNSFYVYCKGPCQRVQPGKLRVQCSTCRQATLTLTQGPSCWDDVLIPNRMSGECQSPHCPGTSAEFFFKCGAHPTSDKETSVALHLIATNSRNITCITCTDVRSPVLVFQCNSRHVICLDCFHLYCVTRLNDRQFVHDPQLGYSLPCVAGCPNSLIKELHHFRILGEEQYNRYQQYGAEECVLQMGGVLCPRPGCGAGLLPEPDQRKVTCEGGNGLGCGFAFCRECKEAYHEGECSAVFEASGTTTQAYRVDERAAEQARWEAASKETIKKTTKPCPRCHVPVEKNGGCMHMKCPQPQCRLEWCWNCGCEWNRVCMGDHWFDV ospg-ube2l3-1 3266374 N/A 3266381 unspecified role unspecified role unspecified role unspecified role Region 26-196 Region 26-196 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3266382 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ospg-ub-1 3266375 N/A 3266381 unspecified role unspecified role unspecified role unspecified role Region 26-196 Region 26-196 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3266383 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3266382 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false park2-ub-2 3266376 N/A 3266384 unspecified role unspecified role unspecified role unspecified role Region 77-465 Region 77-465 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3266383 unspecified role unspecified role unspecified role unspecified role His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false ecolx Escherichia coli direct interaction direct interaction false false false ospg-ub-2 3266377 N/A 3266381 unspecified role unspecified role unspecified role unspecified role Region 26-196 Region 26-196 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3266383 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3266382 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false ospg-ub-3 3266378 N/A 3266381 unspecified role unspecified role unspecified role unspecified role Region 26-196 Region 26-196 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3266383 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ospg-ub-4 3266379 N/A 3266381 unspecified role unspecified role unspecified role unspecified role Region 26-196 Region 26-196 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3266383 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3266382 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ospg-ub-5 3266380 N/A 3266381 unspecified role unspecified role unspecified role unspecified role Region 26-196 Region 26-196 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3266383 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false