DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ sato-2014-1 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Sato Y., Maekawa S., Ishii R., Sanada M., Morikawa T., Shiraishi Y., Yoshida K., Nagata Y., Sato-Otsubo A., Yoshizato T., Suzuki H., Shiozawa Y., Kataoka K., Kon A., Aoki K., Chiba K., Tanaka H., Kume H., Miyano S., Fukayama M., Nureki O., Homma Y., Ogawa S. DIP original experiment: DIP-264632X Science (New York, N.Y.) 2014 IMEx Recurrent somatic mutations underlie corticotropin-independent Cushing's syndrome. Sato Y., Maekawa S., Ishii R., Sanada M., Morikawa T., Shiraishi Y., Yoshida K., Nagata Y., Sato-Otsubo A., Yoshizato T., Suzuki H., Shiozawa Y., Kataoka K., Kon A., Aoki K., Chiba K., Tanaka H., Kume H., Miyano S., Fukayama M., Nureki O., Homma Y., Ogawa S. Only protein-protein interactions imex curation sato-2014-2 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sato Y., Maekawa S., Ishii R., Sanada M., Morikawa T., Shiraishi Y., Yoshida K., Nagata Y., Sato-Otsubo A., Yoshizato T., Suzuki H., Shiozawa Y., Kataoka K., Kon A., Aoki K., Chiba K., Tanaka H., Kume H., Miyano S., Fukayama M., Nureki O., Homma Y., Ogawa S. DIP original experiment: DIP-264633X Science (New York, N.Y.) 2014 IMEx Recurrent somatic mutations underlie corticotropin-independent Cushing's syndrome. Sato Y., Maekawa S., Ishii R., Sanada M., Morikawa T., Shiraishi Y., Yoshida K., Nagata Y., Sato-Otsubo A., Yoshizato T., Suzuki H., Shiozawa Y., Kataoka K., Kon A., Aoki K., Chiba K., Tanaka H., Kume H., Miyano S., Fukayama M., Nureki O., Homma Y., Ogawa S. Only protein-protein interactions imex curation kap0_human cAMP-dependent protein kinase type I-alpha regulatory subunit Tissue-specific extinguisher 1 PRKAR1A PKR1 PRKAR1 TSE1 protein protein human Homo sapiens Human MESGSTAASEEARSLRECELYVQKHNIQALLKDSIVQLCTARPERPMAFLREYFERLEKEEAKQIQNLQKAGTRTDSREDEISPPPPNPVVKGRRRRGAISAEVYTEEDAASYVRKVIPKDYKTMAALAKAIEKNVLFSHLDDNERSDIFDAMFSVSFIAGETVIQQGDEGDNFYVIDQGETDVYVNNEWATSVGEGGSFGELALIYGTPRAATVKAKTNVKLWGIDRDSYRRILMGSTLRKRKMYEEFLSKVSILESLDKWERLTVADALEPVQFEDGQKIVVQGEPGDEFFIILEGSAAVLQRRSENEEFVEVGRLGPSDYFGEIALLMNRPRAATVVARGPLKCVKLDRPRFERVLGPCSDILKRNIQQYNSFVSLSV kapca_human cAMP-dependent protein kinase catalytic subunit alpha PRKACA PKACA protein protein human Homo sapiens Human MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF prkar1a-prkaca-4 3266359 N/A 3266361 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3266362 unspecified role unspecified role prey prey P17612:p.Leu206Arg L206R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli direct interaction direct interaction false false false prkaca-prkar1a-9 3266360 N/A 3266361 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3266362 unspecified role unspecified role bait bait P17612:p.Leu206Arg L206R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false