DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ nuez-2014-1 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. DIP original experiment: DIP-260895X Nature structural & molecular biology 2014 IMEx Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity. Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. Only protein-protein interactions imex curation nuez-2014-2 ecolx Escherichia coli anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. DIP original experiment: DIP-260887X Nature structural & molecular biology 2014 IMEx Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity. Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. Only protein-protein interactions imex curation nuez-2014-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. DIP original experiment: DIP-260889X Nature structural & molecular biology 2014 IMEx Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity. Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. Only protein-protein interactions imex curation nuez-2014-4 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. DIP original experiment: DIP-260892X Nature structural & molecular biology 2014 IMEx Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity. Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. Only protein-protein interactions imex curation nuez-2014-5 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. DIP original experiment: DIP-260890X Nature structural & molecular biology 2014 IMEx Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity. Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. Only protein-protein interactions imex curation nuez-2014-6 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. DIP original experiment: DIP-260893X Nature structural & molecular biology 2014 IMEx Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity. Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. Only protein-protein interactions imex curation nuez-2014-7 ecolx Escherichia coli anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. DIP original experiment: DIP-260888X Nature structural & molecular biology 2014 IMEx Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity. Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. Only protein-protein interactions imex curation nuez-2014-8 ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. DIP original experiment: DIP-260894X Nature structural & molecular biology 2014 IMEx Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity. Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. Only protein-protein interactions imex curation nuez-2014-9 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. DIP original experiment: DIP-260891X Nature structural & molecular biology 2014 IMEx Cas1-Cas2 complex formation mediates spacer acquisition during CRISPR-Cas adaptive immunity. Nuñez JK., Kranzusch PJ., Noeske J., Wright AV., Davies CW., Doudna JA. Only protein-protein interactions imex curation cas1_ecoli CRISPR-associated endonuclease Cas1 ygbT cas1 JW2725 b2755 protein protein ecoli Escherichia coli (strain K12) MTWLPLNPIPLKDRVSMIFLQYGQIDVIDGAFVLIDKTGIRTHIPVGSVACIMLEPGTRVSHAAVRLAAQVGTLLVWVGEAGVRVYASGQPGGARSDKLLYQAKLALDEDLRLKVVRKMFELRFGEPAPARRSVEQLRGIEGSRVRATYALLAKQYGVTWNGRRYDPKDWEKGDTINQCISAATSCLYGVTEAAILAAGYAPAIGFVHTGKPLSFVYDIADIIKFDTVVPKAFEIARRNPGEPDREVRLACRDIFRSSKTLAKLIPLIEDVLAAGEIQPPAPPEDAQPVAIPLPVSLGDAGHRSS cas2_ecoli CRISPR-associated endoribonuclease Cas2 ygbF cas2 JW5438 b2754 protein protein ecoli Escherichia coli (strain K12) MSMLVVVTENVPPRLRGRLAIWLLEVRAGVYVGDVSAKIREMIWEQIAGLAEEGNVVMAWATNTETGFEFQTFGLNRRTPVDLDGLRLVSFLPV ygbt-1 3265718 N/A 3265727 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ygbt-ygbf-1 3265719 N/A 3265728 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3265727 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Q46896:p.Arg252Glu R252E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli physical association physical association false false false ygbf-ygbt-1 3265720 N/A 3265728 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3265727 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ygbf-ygbt-2 3265721 N/A 3265728 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3265727 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ygbf-ygbt-3 3265722 N/A 3265728 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3265727 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ygbt-2 3265723 N/A 3265727 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ygbf-ygbt-4 3265724 N/A 3265728 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region74-94 Region74-94 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3265727 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli physical association physical association false false false ygbf-1 3265725 N/A 3265728 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ygbf-ygbt-5 3265726 N/A 3265728 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3265727 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false