DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ du-2014-1 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Du D., Wang Z., James NR., Voss JE., Klimont E., Ohene-Agyei T., Venter H., Chiu W., Luisi BF. DIP original experiment: DIP-260849X Nature 2014 IMEx Structure of the AcrAB-TolC multidrug efflux pump. Du D., Wang Z., James NR., Voss JE., Klimont E., Ohene-Agyei T., Venter H., Chiu W., Luisi BF. Only protein-protein interactions imex curation du-2014-2 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Du D., Wang Z., James NR., Voss JE., Klimont E., Ohene-Agyei T., Venter H., Chiu W., Luisi BF. DIP original experiment: DIP-260850X Nature 2014 IMEx Structure of the AcrAB-TolC multidrug efflux pump. Du D., Wang Z., James NR., Voss JE., Klimont E., Ohene-Agyei T., Venter H., Chiu W., Luisi BF. Only protein-protein interactions imex curation du-2014-3 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Du D., Wang Z., James NR., Voss JE., Klimont E., Ohene-Agyei T., Venter H., Chiu W., Luisi BF. DIP original experiment: DIP-260851X Nature 2014 IMEx Structure of the AcrAB-TolC multidrug efflux pump. Du D., Wang Z., James NR., Voss JE., Klimont E., Ohene-Agyei T., Venter H., Chiu W., Luisi BF. Only protein-protein interactions imex curation acrz_ecoli Multidrug efflux pump accessory protein AcrZ acrZ AcrAB-TolC multidrug efflux pump accessory protein AcrZ Acridine resistance protein Z ybhT JW5102 b0762 protein protein ecoli Escherichia coli (strain K12) MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQPGQNH acrb_ecoli Multidrug efflux pump subunit AcrB acrB acrE b0462 JW0451 AcrAB-TolC multidrug efflux pump subunit AcrB Acridine resistance protein B protein protein ecoli Escherichia coli (strain K12) MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVTISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADIAQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAANMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQLGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYDIIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVKISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINTLTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLSAVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEGKKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPDEDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNTGIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDFELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALGVSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQMVPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVGYDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALLAATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRLRPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVRRRFSRKNEDIEHSHTVDHH acrb-acrz-1 3265521 N/A 3265524 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3265525 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false acrb-acrz-2 3265522 N/A 3265524 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3265525 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false acrz-acrb-1 3265523 N/A 3265524 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3265525 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association false false false