DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ wang-2014-26 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip de novo protein sequence de novo protein sequencing by mass spectrometry de novo protein sequence Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259806X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions imex curation wang-2014-27 in vitro In vitro hydroxylase assay hydroxylase assay predetermined predetermined participant predetermined Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259810X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions imex curation wang-2014-28 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259814X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions imex curation wang-2014-29 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259808X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions imex curation wang-2014-30 in vitro In vitro hydroxylase assay hydroxylase assay predetermined predetermined participant predetermined Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259812X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions imex curation wang-2014-31 in vitro In vitro hydroxylase assay hydroxylase assay predetermined predetermined participant predetermined Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259811X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions imex curation wang-2014-32 in vitro In vitro pull down pull down autoradiography autoradiography Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259809X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions imex curation wang-2014-33 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259813X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions imex curation wang-2014-34 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. DIP original experiment: DIP-259807X PLoS biology 2014 IMEx JMJD6 promotes colon carcinogenesis through negative regulation of p53 by hydroxylation. Wang F., He L., Huangyang P., Liang J., Si W., Yan R., Han X., Liu S., Gui B., Li W., Miao D., Jing C., Liu Z., Pei F., Sun L., Shang Y. Only protein-protein interactions imex curation q9y383-1 Putative RNA-binding protein Luc7-like 2 LUC7L2 CGI-59 CGI-74 protein protein human Homo sapiens Human MSAQAQMRAMLDQLMGTSRDGDTTRQRIKFSDDRVCKSHLLNCCPHDVLSGTRMDLGECLKVHDLALRADYEIASKEQDFFFELDAMDHLQSFIADCDRRTEVAKKRLAETQEEISAEVAAKAERVHELNEEIGKLLAKVEQLGAEGNVEESQKVMDEVEKARAKKREAEEVYRNSMPASSFQQQKLRVCEVCSAYLGLHDNDRRLADHFGGKLHLGFIEIREKLEELKRVVAEKQEKRNQERLKRREEREREEREKLRRSRSHSKNPKRSRSREHRRHRSRSMSRERKRRTRSKSREKRHRHRSRSSSRSRSRSHQRSRHSSRDRSRERSKRRSSKERFRDQDLASCDRDRSSRDRSPRDRDRKDKKRSYESANGRSEDRRSSEEREAGEI jmjd6_human Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6 PTDSR KIAA0585 JMJD6 Histone arginine demethylase JMJD6 JmjC domain-containing protein 6 Jumonji domain-containing protein 6 Lysyl-hydroxylase JMJD6 Peptide-lysine 5-dioxygenase JMJD6 Phosphatidylserine receptor PSR protein protein human Homo sapiens Human MNHKSKKRIREAKRSARPELKDSLDWTRHNYYESFSLSPAAVADNVERADALQLSVEEFVERYERPYKPVVLLNAQEGWSAQEKWTLERLKRKYRNQKFKCGEDNDGYSVKMKMKYYIEYMESTRDDSPLYIFDSSYGEHPKRRKLLEDYKVPKFFTDDLFQYAGEKRRPPYRWFVMGPPRSGTGIHIDPLGTSAWNALVQGHKRWCLFPTSTPRELIKVTRDEGGNQQDEAITWFNVIYPRTQLPTWPPEFKPLEILQKPGETVFVPGGWWHVVLNLDTTIAITQNFASSTNFPVVWHKTVRGRPKLSRKWYRILKQEHPELAVLADSVDLQESTGIASDSSSDSSSSSSSSSSDSDSECESGSEGDGTVHRRKKRRTCSMVGNGDTTSQDDCVSKERSSSR mint homomint Arginine demethylase which demethylates histone H3 at 'Arg-3' and histone H4 at 'Arg-4'. Required for differentiation of multiple organs during embryogenesis. Probably acts as a key regulator of hematopoietic differentiation. Seems to be necessary for the regulation of macrophage cytokine responses roa2_human Heterogeneous nuclear ribonucleoproteins A2/B1 HNRNPA2B1 HNRPA2B1 protein protein human Homo sapiens Human MEKTLETVPLERKKREKEQFRKLFIGGLSFETTEESLRNYYEQWGKLTDCVVMRDPASKRSRGFGFVTFSSMAEVDAAMAARPHSIDGRVVEPKRAVAREESGKPGAHVTVKKLFVGGIKEDTEEHHLRDYFEEYGKIDTIEIITDRQSGKKRGFGFVTFDDHDPVDKIVLQKYHTINGHNAEVRKALSRQEMQEVQSSRSGRGGNFGFGDSRGGGGNFGPGPGSNFRGGSDGYGSGRGFGDGYNGYGGGPGGGNFGGSPGYGGGRGGYGGGGPGYGNQGGGYGGGYDNYGGGNYGSGNYNDFGNYNQQPSNYGPMKSGNFGGSRNMGGPYGGGNYGPGGSGGSGGYGGRSRY q16630-1 Cleavage and polyadenylation specificity factor subunit 6 CPSF6 CFIM68 Cleavage and polyadenylation specificity factor 68 kDa subunit Pre-mRNA cleavage factor Im 68 kDa subunit Protein HPBRII-4/7 Cleavage factor Im complex 68 kDa subunit protein protein human Homo sapiens Human MADGVDHIDIYADVGEEFNQEAEYGGHDQIDLYDDVISPSANNGDAPEDRDYMDTLPPTVGDDVGKGAAPNVVYTYTGKRIALYIGNLTWWTTDEDLTEAVHSLGVNDILEIKFFENRANGQSKGFALVGVGSEASSKKLMDLLPKRELHGQNPVVTPCNKQFLSQFEMQSRKTTQSGQMSGEGKAGPPGGSSRAAFPQGGRGRGRFPGAVPGGDRFPGPAGPGGPPPPFPAGQTPPRPPLGPPGPPGPPGPPPPGQVLPPPLAGPPNRGDRPPPPVLFPGQPFGQPPLGPLPPGPPPPVPGYGPPPGPPPPQQGPPPPPGPFPPRPPGPLGPPLTLAPPPHLPGPPPGAPPPAPHVNPAFFPPPTNSGMPTSDSRGPPPTDPYGRPPPYDRGDYGPPGREMDTARTPLSEAEFEEIMNRNRAISSSAISRAVSDASAGDYGSAIETLVTAISLIKQSKVSADDRCKVLISSLQDCLHGIESKSYGSGSRRERSRERDHSRSREKSRRHKSRSRDRHDDYYRERSRERERHRDRDRDRDRERDREREYRHR p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD mdm4_human Protein Mdm4 MDM4 MDMX p53-binding protein Mdm4 Mdm2-like p53-binding protein Protein Mdmx Double minute 4 protein protein protein human Homo sapiens Human MTSFSTSAQCSTSDSACRISPGQINQVRPKLPLLKILHAAGAQGEMFTVKEVMHYLGQYIMVKQLYDQQEQHMVYCGGDLLGELLGRQSFSVKDPSPLYDMLRKNLVTLATATTDAAQTLALAQDHSMDIPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLIENLAQDETSRLDLGFEEWDVAGLPWWFLGNLRSNYTPRSNGSTDLQTNQDVGTAIVSDTTDDLWFLNESVSEQLGVGIKVEAADTEQTSEEVGKVSDKKVIEVGKNDDLEDSKSLSDDTDVEVTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCSKLTHSLSTSDITAIPEKENEGNDVPDCRRTISAPVVRPKDAYIKKENSKLFDPCNSVEFLDLAHSSESQETISSMGEQLDNLSEQRTDTENMEDCQNLLKPCSLCEKRPRDGNIIHGRTGHLVTCFHCARRLKKAGASCPICKKEIQLVIKVFIA mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG jmjd6-cpsf6-1 3263882 N/A 3263891 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3263892 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3263893 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3263894 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3263895 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false jmjd6-tp53-1 3263883 N/A 3263892 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli N/A 3263895 enzyme target enzyme target substrate substrate unspecified role unspecified role K382OH K382OH hy1lys monohydroxylated lysine Hy1Lys Hydroxy Lysyl (-Hyl-) Oxidation hydroxylationk modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli hydroxylation hydroxylation reaction hydroxylation false false false tp53-mdm2-33 3263884 N/A 3263896 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3263897 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3263895 unspecified role unspecified role bait bait human Homo sapiens Human association association false false false tp53-jmjd6-1 3263885 N/A 3263892 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3263895 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false jmjd6-tp53-2 3263886 N/A 3263892 enzyme enzyme unspecified role unspecified role Q6NYC1:p.[His187Ala;Asp189Ala] H187A/D189A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3263895 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 381-393 Region 381-393 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false K382OH K382OH hy1lys monohydroxylated lysine Hy1Lys Hydroxy Lysyl (-Hyl-) Oxidation hydroxylationk modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false in vitro In vitro hydroxylation hydroxylation reaction hydroxylation false false false jmjd6-tp53-3 3263887 N/A 3263892 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli N/A 3263895 enzyme target enzyme target substrate substrate unspecified role unspecified role K382OH K382OH hy1lys monohydroxylated lysine Hy1Lys Hydroxy Lysyl (-Hyl-) Oxidation hydroxylationk modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain false Region 381-393 Region 381-393 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P04637:p.Lys382Ala K382A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P04637:p.Lys382Arg K382R mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false K382ac K382ac naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain false K382me1 K382me1 me1lys monomethylated L-lysine Me1Lys Methyl Methylation methylk certain certain sequence position certain certain certain sequence position certain false in vitro In vitro hydroxylation hydroxylation reaction hydroxylation false false false jmjd6-tp53-4 3263888 N/A 3263892 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3263895 unspecified role unspecified role prey prey Region 290-393 Region 290-393 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false tp53-ub-15 3263889 N/A 3263898 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false human Homo sapiens Human N/A 3263895 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false jmjd6-tp53-5 3263890 N/A 3263892 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3263895 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false