DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ sakamaki-2014-1 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. DIP original experiment: DIP-260113X Nature cell biology 2014 IMEx Role of the SIK2-p35-PJA2 complex in pancreatic β-cell functional compensation. Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. Only protein-protein interactions imex curation sakamaki-2014-2 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. DIP original experiment: DIP-260121X Nature cell biology 2014 IMEx Role of the SIK2-p35-PJA2 complex in pancreatic β-cell functional compensation. Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. Only protein-protein interactions imex curation sakamaki-2014-3 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. DIP original experiment: DIP-260118X Nature cell biology 2014 IMEx Role of the SIK2-p35-PJA2 complex in pancreatic β-cell functional compensation. Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. Only protein-protein interactions imex curation sakamaki-2014-4 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. DIP original experiment: DIP-260116X Nature cell biology 2014 IMEx Role of the SIK2-p35-PJA2 complex in pancreatic β-cell functional compensation. Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. Only protein-protein interactions imex curation sakamaki-2014-5 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. DIP original experiment: DIP-260119X Nature cell biology 2014 IMEx Role of the SIK2-p35-PJA2 complex in pancreatic β-cell functional compensation. Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. Only protein-protein interactions imex curation sakamaki-2014-6 in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. DIP original experiment: DIP-260114X Nature cell biology 2014 IMEx Role of the SIK2-p35-PJA2 complex in pancreatic β-cell functional compensation. Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. Only protein-protein interactions imex curation sakamaki-2014-7 mouse Mus musculus Mouse anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. DIP original experiment: DIP-260117X Nature cell biology 2014 IMEx Role of the SIK2-p35-PJA2 complex in pancreatic β-cell functional compensation. Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. Only protein-protein interactions imex curation sakamaki-2014-8 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. DIP original experiment: DIP-260115X Nature cell biology 2014 IMEx Role of the SIK2-p35-PJA2 complex in pancreatic β-cell functional compensation. Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. Only protein-protein interactions imex curation sakamaki-2014-9 mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. DIP original experiment: DIP-260120X Nature cell biology 2014 IMEx Role of the SIK2-p35-PJA2 complex in pancreatic β-cell functional compensation. Sakamaki J., Fu A., Reeks C., Baird S., Depatie C., Al Azzabi M., Bardeesy N., Gingras AC., Yee SP., Screaton RA. Only protein-protein interactions imex curation sik2_mouse Serine/threonine-protein kinase SIK2 Sik2 Snf1lk2 Salt-inducible kinase 2 Serine/threonine-protein kinase SNF1-like kinase 2 protein protein mouse Mus musculus Mouse MVMADGPRHLQRGPVRVGFYDIEGTLGKGNFAVVKLGRHRTTKTEVAIKIIDKSQLDAVNLEKIYREVQIMKMLDHPHIIKLYQVMETKSMLYLVTEYAKNGEIFDYLANHGRLNESEARRKFWQILSAVDYCHGRKVVHRDLKAENLLLDNNMNIKIADFGFGNFFKTGELLATWCGSPPYAAPEVFEGQQYEGPQLDIWSMGVVLYVLVCGALPFDGPTLPILRQRVLEGRFRIPYFMSEDCEHLIRRMLVLDPSKRLSIAQIKEHKWMLIEVPVQRPILYPQEQENEPSIGEFNEQVLRLMHSLGIDQQKTVESLQNKSYNHFAAIYFLLVERLKSHRSSFPVEQRLDGRQRRPSTIAEQTVAKAQTVGLPVTLHPPNVRLMRSTLLPQASNVEAFSFPTSSCQAEAAFMEEECVDTPKVNGCLLDPVPPVLVRKGCQSLPSSMMETSIDEGLETEGEAEEDPSQAFEAFQATRSGQRRHTLSEVTNQLVVMPGAGKMFSMSDNPSLESVDSEYDMGSAQRDLNFLEDSPSLKDIMLANQPSPRMTSPFISLRPANPAMQALSSQKREAHNRSPVSFREGRRASDTSLTQGIVAFRQHLQNLARTKGILELNKVQLLYEQMGSNADPTLTSTAPQLQDLSSSCPQEEISQQQESVSSLSASMHPQLSPQQSLETQYLQHRLQKPNLLPKAQSPCPVYCKEPPRSLEQQLQEHRLQQKRLFLQKQSQLQAYFNQMQIAESSYPGPSQQLALPHQETPLTSQQPPSFSLTQALSPVLEPSSEQMQFSSFLSQYPEMQLQPLPSTPGPRAPPPLPSQLQQHQQPPPPPPPPPPQQPGAAPTSLQFSYQTCELPSTTSSVPNYPASCHYPVDGAQQSNLTGADCPRSSGLQDTASSYDPLALSELPGLFDCEMVEAVDPQHNGVVSCLARET cd5r1_mouse Cyclin-dependent kinase 5 activator 1 Cdk5r1 Cdk5r Nck5a Cyclin-dependent kinase 5 regulatory subunit 1 TPKII regulatory subunit protein protein mouse Mus musculus Mouse MGTVLSLSPSYRKATLFEDGAATVGHYTAVQNSKNAKDKNLKRHSIISVLPWKRIVAVSAKKKNSKKAQPNSSYQSNIAHLNNENLKKSLSCANLSTFAQPPPAQPPAPPASQLSGSQTGVSSSVKKAPHPAITSAGTPKRVIVQASTSELLRCLGEFLCRRCYRLKHLSPTDPVLWLRSVDRSLLLQGWQDQGFITPANVVFLYMLCRDVISSEVGSDHELQAVLLTCLYLSYSYMGNEISYPLKPFLVESCKEAFWDRCLSVINLMSSKMLQINADPHYFTQVFSDLKNESGQEDKKRLLLGLDR p35 is a neuron specific activator of CDK5. The complex p35/CDK5 is required for neurite outgrowth and cortical lamination. Activator of TPKII mint pja2_human E3 ubiquitin-protein ligase Praja-2 PJA2 KIAA0438 RNF131 RING finger protein 131 RING-type E3 ubiquitin transferase Praja-2 protein protein human Homo sapiens Human MSQYTEKEPAAMDQESGKAVWPKPAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDDVPKENSSGSSPLDQVDSSLPSEPIFEKSETEIPTCGSALNQTTESSQSFVAVHHSEEGRDTLGSSTNLHNHSEGEYIPGACSASSVQNGIALVHTDSYDPDGKHGEDNDHLQLSAEVVEGSRYQESLGNTVFELENREAEAYTGLSPPVPSFNCEVRDEFEELDSVPLVKSSAGDTEFVHQNSQEIQRSSQDEMVSTKQQNNTSQERQTEHSPEDAACGPGHICSEQNTNDREKNHGSSPEQVVRPKVRKLISSSQVDQETGFNRHEAKQRSVQRWREALEVEESGSDDLLIKCEEYDGEHDCMFLDPPYSRVITQRETENNQMTSESGATAGRQEVDNTFWNGCGDYYQLYDKDEDSSECSDGEWSASLPHRFSGTEKDQSSSDESWETLPGKDENEPELQSDSSGPEEENQELSLQEGEQTSLEEGEIPWLQYNEVNESSSDEGNEPANEFAQPAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDHTAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVIEASAAPSSEPDPDAPPSNDSIAEAP ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG pja2_mouse E3 ubiquitin-protein ligase Praja-2 Pja2 Kiaa0438 Rnf131 RING finger protein 131 RING-type E3 ubiquitin transferase Praja-2 protein protein mouse Mus musculus Mouse MSQYTEKEPSVMDQESSKAAWPKPAGGYQTITGRRYGRRHAYVSFKPCMTRHERSLGRAGDDYEVLELDDVPKENTSGSSSLDQVHPALPNEPTVEKSETEISTCGPALNQSTESSPSIATVCHSEEVRETLESNTNLHNRTETEHTPAVCNVSSVQNGIMLVHTDSYDPDSKHDENGSLQLGAEAVEGGRHQKGLGRAVFELENGEAEIYADLSPSVPSLNGEISEAFEELDSAPLEKSSTADAELVHQNGQEFQRSSEDGVVRKRRQDDTDQGRQTENSTEDADCAPGHVEQNTSDRANHHGSSPEQVVRPKVRKVISSSQVDQEIGFNRHEAKQRSVQRWREALEVEECSSDDPIIKCDDYDGDHDCMFLTPTYSRVTQRETERNRVTSENGATASGRQESRDNAFWNACGEYYQLFDKDEDSSECSDGEWSASLPHRFSGTEKDQSSSDDSWETLPGKDENDPELQSDSSGPEEENQELSLQEGEQTSLEEGEIPWLQYNEVNESSSDEGNEPANEFAQPEAFMLDGNNNLEDDSSVSEDLDVDWSLFDGFADGLGVAEAISYVDPQFLTYMALEERLAQAMETALAHLESLAVDVEVANPPASKESIDGLPETLVLEDHTAIGQEQCCPICCSEYIKDDIATELPCHHFFHKPCVSIWLQKSGTCPVCRRHFPPAVIDASAAASSDPDPDASPANDNAEEAP ubiq_mouse Ubiquitin Rps27a Uba80 Ubcep1 Uba52 Ubcep2 Ubb Ubc protein protein mouse Mus musculus Mouse MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG sik2-6 3263176 N/A 3263185 putative self putative self unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation false false false pja2-cdk5r1-1 3263177 N/A 3263186 unspecified role unspecified role prey prey V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3263187 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false cdk5r1-sik2-1 3263178 N/A 3263186 unspecified role unspecified role bait bait mouse Mus musculus Mouse N/A 3263185 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association false false false cdk5r1-hub-1 3263179 N/A 3263186 unspecified role unspecified role bait bait V5-tag V5-tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3263188 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false sik2-cdk5r1-1 3263180 N/A 3263186 unspecified role unspecified role prey prey mouse Mus musculus Mouse N/A 3263185 unspecified role unspecified role bait bait mouse Mus musculus Mouse physical association physical association false false false cdk5r1-sik2-2 3263181 N/A 3263186 enzyme target enzyme target substrate substrate unspecified role unspecified role P61809:p.Ser91Ala S91A mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3263185 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation false false false cdk5r1-pja2-1 3263182 N/A 3263186 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false mouse Mus musculus Mouse N/A 3263189 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association false false false cdk5r1-mub-1 3263183 N/A 3263186 unspecified role unspecified role bait bait mouse Mus musculus Mouse N/A 3263190 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association false false false cdk5r1-pja2-2 3263184 N/A 3263186 unspecified role unspecified role bait bait mouse Mus musculus Mouse N/A 3263189 unspecified role unspecified role prey prey mouse Mus musculus Mouse physical association physical association false false false