DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ timsah-2014-1 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. DIP original experiment: DIP-260049X Nature structural & molecular biology 2014 IMEx Competition between Grb2 and Plcγ1 for FGFR2 regulates basal phospholipase activity and invasion. Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. Only protein-protein interactions imex curation timsah-2014-2 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. DIP original experiment: DIP-260041X Nature structural & molecular biology 2014 IMEx Competition between Grb2 and Plcγ1 for FGFR2 regulates basal phospholipase activity and invasion. Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. Only protein-protein interactions imex curation timsah-2014-3 in vitro In vitro mst microscale thermophoresis mst predetermined predetermined participant predetermined Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. DIP original experiment: DIP-260047X Nature structural & molecular biology 2014 IMEx Competition between Grb2 and Plcγ1 for FGFR2 regulates basal phospholipase activity and invasion. Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. Only protein-protein interactions imex curation timsah-2014-4 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. DIP original experiment: DIP-260045X Nature structural & molecular biology 2014 IMEx Competition between Grb2 and Plcγ1 for FGFR2 regulates basal phospholipase activity and invasion. Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. Only protein-protein interactions imex curation timsah-2014-5 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. DIP original experiment: DIP-260044X Nature structural & molecular biology 2014 IMEx Competition between Grb2 and Plcγ1 for FGFR2 regulates basal phospholipase activity and invasion. Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. Only protein-protein interactions imex curation timsah-2014-6 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. DIP original experiment: DIP-260042X Nature structural & molecular biology 2014 IMEx Competition between Grb2 and Plcγ1 for FGFR2 regulates basal phospholipase activity and invasion. Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. Only protein-protein interactions imex curation timsah-2014-7 in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. DIP original experiment: DIP-260048X Nature structural & molecular biology 2014 IMEx Competition between Grb2 and Plcγ1 for FGFR2 regulates basal phospholipase activity and invasion. Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. Only protein-protein interactions imex curation timsah-2014-8 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. DIP original experiment: DIP-260046X Nature structural & molecular biology 2014 IMEx Competition between Grb2 and Plcγ1 for FGFR2 regulates basal phospholipase activity and invasion. Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. Only protein-protein interactions imex curation timsah-2014-9 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. DIP original experiment: DIP-260043X Nature structural & molecular biology 2014 IMEx Competition between Grb2 and Plcγ1 for FGFR2 regulates basal phospholipase activity and invasion. Timsah Z., Ahmed Z., Lin CC., Melo FA., Stagg LJ., Leonard PG., Jeyabal P., Berrout J., O'Neil RG., Bogdanov M., Ladbury JE. Only protein-protein interactions imex curation plcg1_human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 PLC-148 PLC1 Phospholipase C-II PLCG1 Phosphoinositide phospholipase C-gamma-1 Phospholipase C-gamma-1 protein protein human Homo sapiens Human MAGAASPCANGCGPGAPSDAEVLHLCRSLEVGTVMTLFYSKKSQRPERKTFQVKLETRQITWSRGADKIEGAIDIREIKEIRPGKTSRDFDRYQEDPAFRPDQSHCFVILYGMEFRLKTLSLQATSEDEVNMWIKGLTWLMEDTLQAPTPLQIERWLRKQFYSVDRNREDRISAKDLKNMLSQVNYRVPNMRFLRERLTDLEQRSGDITYGQFAQLYRSLMYSAQKTMDLPFLEASTLRAGERPELCRVSLPEFQQFLLDYQGELWAVDRLQVQEFMLSFLRDPLREIEEPYFFLDEFVTFLFSKENSVWNSQLDAVCPDTMNNPLSHYWISSSHNTYLTGDQFSSESSLEAYARCLRMGCRCIELDCWDGPDGMPVIYHGHTLTTKIKFSDVLHTIKEHAFVASEYPVILSIEDHCSIAQQRNMAQYFKKVLGDTLLTKPVEISADGLPSPNQLKRKILIKHKKLAEGSAYEEVPTSMMYSENDISNSIKNGILYLEDPVNHEWYPHYFVLTSSKIYYSEETSSDQGNEDEEEPKEVSSSTELHSNEKWFHGKLGAGRDGRHIAERLLTEYCIETGAPDGSFLVRESETFVGDYTLSFWRNGKVQHCRIHSRQDAGTPKFFLTDNLVFDSLYDLITHYQQVPLRCNEFEMRLSEPVPQTNAHESKEWYHASLTRAQAEHMLMRVPRDGAFLVRKRNEPNSYAISFRAEGKIKHCRVQQEGQTVMLGNSEFDSLVDLISYYEKHPLYRKMKLRYPINEEALEKIGTAEPDYGALYEGRNPGFYVEANPMPTFKCAVKALFDYKAQREDELTFIKSAIIQNVEKQEGGWWRGDYGGKKQLWFPSNYVEEMVNPVALEPEREHLDENSPLGDLLRGVLDVPACQIAIRPEGKNNRLFVFSISMASVAHWSLDVAADSQEELQDWVKKIREVAQTADARLTEGKIMERRKKIALELSELVVYCRPVPFDEEKIGTERACYRDMSSFPETKAEKYVNKAKGKKFLQYNRLQLSRIYPKGQRLDSSNYDPLPMWICGSQLVALNFQTPDKPMQMNQALFMTGRHCGYVLQPSTMRDEAFDPFDKSSLRGLEPCAISIEVLGARHLPKNGRGIVCPFVEIEVAGAEYDSTKQKTEFVVDNGLNPVWPAKPFHFQISNPEFAFLRFVVYEEDMFSDQNFLAQATFPVKGLKTGYRAVPLKNNYSEDLELASLLIKIDIFPAKENGDLSPFSGTSLRERGSDASGQLFHGRAREGSFESRYQQPFEDFRISQEHLADHFDSRERRAPRRTRVNGDNRL p21802-1 Fibroblast growth factor receptor 2 K-sam Keratinocyte growth factor receptor BEK FGFR2IIIc FGFR2 BEK KGFR KSAM protein protein human Homo sapiens Human MVSWGRFICLVVVTMATLSLARPSFSLVEDTTLEPEEPPTKYQISQPEVYVAAPGESLEVRCLLKDAAVISWTKDGVHLGPNNRTVLIGEYLQIKGATPRDSGLYACTASRTVDSETWYFMVNVTDAISSGDDEDDTDGAEDFVSENSNNKRAPYWTNTEKMEKRLHAVPAANTVKFRCPAGGNPMPTMRWLKNGKEFKQEHRIGGYKVRNQHWSLIMESVVPSDKGNYTCVVENEYGSINHTYHLDVVERSPHRPILQAGLPANASTVVGGDVEFVCKVYSDAQPHIQWIKHVEKNGSKYGPDGLPYLKVLKAAGVNTTDKEIEVLYIRNVTFEDAGEYTCLAGNSIGISFHSAWLTVLPAPGREKEITASPDYLEIAIYCIGVFLIACMVVTVILCRMKNTTKKPDFSSQPAVHKLTKRIPLRRQVTVSAESSSSMNSNTPLVRITTRLSSTADTPMLAGVSEYELPEDPKWEFPRDKLTLGKPLGEGCFGQVVMAEAVGIDKDKPKEAVTVAVKMLKDDATEKDLSDLVSEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLRARRPPGMEYSYDINRVPEEQMTFKDLVSCTYQLARGMEYLASQKCIHRDLAARNVLVTENNVMKIADFGLARDINNIDYYKKTTNGRLPVKWMAPEALFDRVYTHQSDVWSFGVLMWEIFTLGGSPYPGIPVEELFKLLKEGHRMDKPANCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRILTLTTNEEYLDLSQPLEQYSPSYPDTRSSCSSGDDSVFSPDPMPYEPCLPQYPHINGSVKT p62993-1 Growth factor receptor-bound protein 2 GRB2 ASH Adapter protein GRB2 Protein Ash SH2/SH3 adapter GRB2 Ash-L protein protein human Homo sapiens Human MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV fgfr2-plcg1-1 3262356 N/A 3262365 unspecified role unspecified role prey prey Region 550-848 Region 550-848 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false RFP-tag RFP-tag rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3262366 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human physical association physical association false false false plcg1-fgfr2-1 3262357 N/A 3262366 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3262365 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false fgfr2-plcg1-2 3262358 N/A 3262365 unspecified role unspecified role unspecified role unspecified role Region 795-848 Region 795-848 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3262366 unspecified role unspecified role unspecified role unspecified role Region 799-821 Region 799-821 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false fgfr2-plcg1-3 3262359 N/A 3262365 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3262366 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region 764-821 Region 764-821 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false fgfr2-plcg1-4 3262360 N/A 3262365 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3262366 unspecified role unspecified role bait bait Region 764-821 Region 764-821 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P21802-1:p.Pro804Ala P804A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P21802-1:p.Pro810Ala P810A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P21802-1:p.Pro813Ala P813A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli physical association physical association false false false fgfr2-plcg1-5 3262361 N/A 3262366 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3262365 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false fgfr2-plcg1-6 3262362 N/A 3262365 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Region 795-848 Region 795-848 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Alexa555-label Alexa555-label alexa label alexa label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3262366 unspecified role unspecified role fluorescence donor fluorescence donor Region 764-821 Region 764-821 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Alexa488-label Alexa488-label alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli direct interaction direct interaction false false false fgfr2-grb2-1 3262363 N/A 3262367 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3262365 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3262366 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false Region 764-821 Region 764-821 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli association association false false false plcg1-fgfr2-2 3262364 N/A 3262366 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3262365 unspecified role unspecified role bait bait Region 795-848 Region 795-848 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli physical association physical association false false false