DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ lim-2013-2 ecolx Escherichia coli anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Lim B., Miyazaki R., Neher S., Siegele DA., Ito K., Walter P., Akiyama Y., Yura T., Gross CA. DIP original experiment: DIP-259716X PLoS biology 2013 IMEx Heat shock transcription factor σ32 co-opts the signal recognition particle to regulate protein homeostasis in E. coli. Lim B., Miyazaki R., Neher S., Siegele DA., Ito K., Walter P., Akiyama Y., Yura T., Gross CA. Only protein-protein interactions imex curation lim-2013-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Lim B., Miyazaki R., Neher S., Siegele DA., Ito K., Walter P., Akiyama Y., Yura T., Gross CA. DIP original experiment: DIP-259719X PLoS biology 2013 IMEx Heat shock transcription factor σ32 co-opts the signal recognition particle to regulate protein homeostasis in E. coli. Lim B., Miyazaki R., Neher S., Siegele DA., Ito K., Walter P., Akiyama Y., Yura T., Gross CA. Only protein-protein interactions imex curation lim-2013-4 ecolx Escherichia coli crosslink cross-linking study crosslink anti tag western anti tag western blot anti tag western Lim B., Miyazaki R., Neher S., Siegele DA., Ito K., Walter P., Akiyama Y., Yura T., Gross CA. DIP original experiment: DIP-259721X PLoS biology 2013 IMEx Heat shock transcription factor σ32 co-opts the signal recognition particle to regulate protein homeostasis in E. coli. Lim B., Miyazaki R., Neher S., Siegele DA., Ito K., Walter P., Akiyama Y., Yura T., Gross CA. Only protein-protein interactions imex curation lim-2013-5 ecolx Escherichia coli anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Lim B., Miyazaki R., Neher S., Siegele DA., Ito K., Walter P., Akiyama Y., Yura T., Gross CA. DIP original experiment: DIP-259717X PLoS biology 2013 IMEx Heat shock transcription factor σ32 co-opts the signal recognition particle to regulate protein homeostasis in E. coli. Lim B., Miyazaki R., Neher S., Siegele DA., Ito K., Walter P., Akiyama Y., Yura T., Gross CA. Only protein-protein interactions imex curation lim-2013-6 in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Lim B., Miyazaki R., Neher S., Siegele DA., Ito K., Walter P., Akiyama Y., Yura T., Gross CA. DIP original experiment: DIP-259720X PLoS biology 2013 IMEx Heat shock transcription factor σ32 co-opts the signal recognition particle to regulate protein homeostasis in E. coli. Lim B., Miyazaki R., Neher S., Siegele DA., Ito K., Walter P., Akiyama Y., Yura T., Gross CA. Only protein-protein interactions imex curation lim-2013-7 in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting western blot western blot Immuno blot Immuno blot Lim B., Miyazaki R., Neher S., Siegele DA., Ito K., Walter P., Akiyama Y., Yura T., Gross CA. DIP original experiment: DIP-259718X PLoS biology 2013 IMEx Heat shock transcription factor σ32 co-opts the signal recognition particle to regulate protein homeostasis in E. coli. Lim B., Miyazaki R., Neher S., Siegele DA., Ito K., Walter P., Akiyama Y., Yura T., Gross CA. Only protein-protein interactions imex curation rpoh_ecoli RNA polymerase sigma factor RpoH Heat shock regulatory protein F33.4 rpoH fam hin htpR b3461 RNA polymerase sigma-32 factor JW3426 protein protein ecoli Escherichia coli (strain K12) MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYHGDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARELGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWEEQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMKKLRAAIEA This sequence has been withdrawn from Uniprot. srp54_ecoli Signal recognition particle protein ffh b2610 Fifty-four homolog p48 JW5414 protein protein ecoli Escherichia coli (strain K12) MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVALPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAVVLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFFPSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETLFVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVGEKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDFLEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPEIIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGFPGR E9C7A7101CC04D66 ftsy_ecoli Signal recognition particle receptor FtsY ftsY b3464 JW3429 protein protein ecoli Escherichia coli (strain K12) MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELETALAAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKAPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED EBE640072B5D1021 seca_ecoli Protein translocase subunit SecA secA azi pea prlD b0098 JW0096 protein protein ecoli Escherichia coli (strain K12) MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELKGKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEMRTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPGMPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDEARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTERGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVIIVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTGTADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAGRGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRRIDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVTKAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIREDVFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERILAQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKDPKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQLSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ ffh-rpoh-1 3261332 N/A 3261338 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3261339 unspecified role unspecified role bait bait ecolx Escherichia coli physical association physical association false false false ffh-rpoh-2 3261333 N/A 3261338 unspecified role unspecified role unspecified role unspecified role P0AGB3:p.Ile54Asn I54N mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3261339 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false ffh-rpoh-3 3261334 N/A 3261338 unspecified role unspecified role unspecified role unspecified role CL-label CL-label cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3261339 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false ftsy-rpoh-1 3261335 N/A 3261340 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3261338 unspecified role unspecified role prey prey ecolx Escherichia coli physical association physical association false false false seca-rpoh-1 3261336 N/A 3261341 unspecified role unspecified role bait bait ecolx Escherichia coli N/A 3261338 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false ffh-rpoh-4 3261337 N/A 3261338 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3261339 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction false false false