DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ huang-2014-1 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Huang H., Ceccarelli DF., Orlicky S., St-Cyr DJ., Ziemba A., Garg P., Plamondon S., Auer M., Sidhu S., Marinier A., Kleiger G., Tyers M., Sicheri F. DIP original experiment: DIP-260175X Nature chemical biology 2014 IMEx E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. Huang H., Ceccarelli DF., Orlicky S., St-Cyr DJ., Ziemba A., Garg P., Plamondon S., Auer M., Sidhu S., Marinier A., Kleiger G., Tyers M., Sicheri F. Only protein-protein interactions imex curation huang-2014-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Huang H., Ceccarelli DF., Orlicky S., St-Cyr DJ., Ziemba A., Garg P., Plamondon S., Auer M., Sidhu S., Marinier A., Kleiger G., Tyers M., Sicheri F. DIP original experiment: DIP-260173X Nature chemical biology 2014 IMEx E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. Huang H., Ceccarelli DF., Orlicky S., St-Cyr DJ., Ziemba A., Garg P., Plamondon S., Auer M., Sidhu S., Marinier A., Kleiger G., Tyers M., Sicheri F. Only protein-protein interactions imex curation huang-2014-3 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Huang H., Ceccarelli DF., Orlicky S., St-Cyr DJ., Ziemba A., Garg P., Plamondon S., Auer M., Sidhu S., Marinier A., Kleiger G., Tyers M., Sicheri F. DIP original experiment: DIP-260174X Nature chemical biology 2014 IMEx E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. Huang H., Ceccarelli DF., Orlicky S., St-Cyr DJ., Ziemba A., Garg P., Plamondon S., Auer M., Sidhu S., Marinier A., Kleiger G., Tyers M., Sicheri F. Only protein-protein interactions imex curation rbx1_human E3 ubiquitin-protein ligase RBX1 RBX1 RNF75 ROC1 Regulator of cullins 1 RING finger protein 75 Protein ZYP RING-box protein 1 E3 ubiquitin-protein transferase RBX1 protein protein human Homo sapiens Human MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH ub2r1_human Ubiquitin-conjugating enzyme E2 R1 Ubiquitin-protein ligase R1 Ubiquitin-conjugating enzyme E2-32 kDa complementing Ubiquitin-conjugating enzyme E2-CDC34 CDC34 UBE2R1 UBCH3 (E3-independent) E2 ubiquitin-conjugating enzyme R1 E2 ubiquitin-conjugating enzyme R1 protein protein human Homo sapiens Human MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG cdc34-rbx1-1 3260987 N/A 3260990 unspecified role unspecified role unspecified role unspecified role Region 12-108 Region 12-108 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3260991 unspecified role unspecified role unspecified role unspecified role P49427:p.Thr117Glu T117E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P49427:p.Tyr70Arg Y70R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cdc34-ub-3 3260988 N/A 3260992 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3260991 unspecified role unspecified role unspecified role unspecified role Region 7-184 Region 7-184 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cdc34-ub-4 3260989 N/A 3260992 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3260991 unspecified role unspecified role unspecified role unspecified role Region 7-184 Region 7-184 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P49427:p.Glu133Arg E133R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P49427:p.Ser129Arg S129R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false