DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ ritter-2013-1 in vitro In vitro pull down pull down predetermined predetermined participant predetermined Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. DIP original experiment: DIP-256110X PLoS biology 2013 IMEx NECAP 1 regulates AP-2 interactions to control vesicle size, number, and cargo during clathrin-mediated endocytosis. Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. Only protein-protein interactions imex curation ritter-2013-2 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. DIP original experiment: DIP-256105X PLoS biology 2013 IMEx NECAP 1 regulates AP-2 interactions to control vesicle size, number, and cargo during clathrin-mediated endocytosis. Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. Only protein-protein interactions imex curation ritter-2013-3 in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. DIP original experiment: DIP-256109X PLoS biology 2013 IMEx NECAP 1 regulates AP-2 interactions to control vesicle size, number, and cargo during clathrin-mediated endocytosis. Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. Only protein-protein interactions imex curation ritter-2013-4 in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. DIP original experiment: DIP-256107X PLoS biology 2013 IMEx NECAP 1 regulates AP-2 interactions to control vesicle size, number, and cargo during clathrin-mediated endocytosis. Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. Only protein-protein interactions imex curation ritter-2013-5 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. DIP original experiment: DIP-256112X PLoS biology 2013 IMEx NECAP 1 regulates AP-2 interactions to control vesicle size, number, and cargo during clathrin-mediated endocytosis. Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. Only protein-protein interactions imex curation ritter-2013-6 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. DIP original experiment: DIP-256108X PLoS biology 2013 IMEx NECAP 1 regulates AP-2 interactions to control vesicle size, number, and cargo during clathrin-mediated endocytosis. Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. Only protein-protein interactions imex curation ritter-2013-7 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. DIP original experiment: DIP-256106X PLoS biology 2013 IMEx NECAP 1 regulates AP-2 interactions to control vesicle size, number, and cargo during clathrin-mediated endocytosis. Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. Only protein-protein interactions imex curation ritter-2013-8 in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. DIP original experiment: DIP-256111X PLoS biology 2013 IMEx NECAP 1 regulates AP-2 interactions to control vesicle size, number, and cargo during clathrin-mediated endocytosis. Ritter B., Murphy S., Dokainish H., Girard M., Gudheti MV., Kozlov G., Halin M., Philie J., Jorgensen EM., Gehring K., McPherson PS. Only protein-protein interactions imex curation ap2a2_mouse AP-2 complex subunit alpha-2 Adaptor protein complex AP-2 subunit alpha-2 Alpha2-adaptin Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit Alpha-adaptin C Clathrin assembly protein complex 2 alpha-C large chain 100 kDa coated vesicle protein C Ap2a2 Adtab Adaptor-related protein complex 2 subunit alpha-2 protein protein mouse Mus musculus Mouse MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIANVGSREMAEAFAGEIPKILVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPDPAVRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAQQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFNLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKATIQDVLRSDSQLKNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEETKRERSIDVNGGPEPVPASTSAASTPSPSADLLGLGAVPPAPTGPPPSSGGGLLVDVFSDSASAVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICADDLQTNLNLQTKPVDPTVDGGAQVQQVVNIECISDFTEAPVLNIQFRYGGTFQNVSVKLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEITKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKDTVSQRLCELLSEQF bin1_rat Myc box-dependent-interacting protein 1 Bin1 Amph2 Amphl Bridging integrator 1 Amphiphysin-like protein Amphiphysin II protein protein rat Rattus norvegicus (Rat) Rat MAEMGSKGVTAGKIASNVQKKLTRAQEKVLQKLGKADETKDEQFEQCVQNFNKQLTEGTRLQKDLRTYLASVKAMHEASKKLSECLQEVYEPEWPGRDEANKIAENNDLLWMDYHQKLVDQALLTMDTYLGQFPDIKSRIAKRGRKLVDYDSARHHYESLQTAKKKDEAKIAKPVSLLEKAAPQWCQGKLQAHLVAQTNLLRNQAEEELIKAQKVFEEMNVDLQEELPSLWNSRVGFYVNTFQSIAGLEENFHKEMSKLNQNLNDVLVSLEKQHGSNTFTVKAQPSDSAPEKGNKSPSPPPDGSPAATPEIRVNHEPEPASGASPGATIPKSPSQLRKGPPVPPPPKHTPSKEMKQEQILSLFDDAFVPEISVTTPSQFEAPGPFSEQASLLDLDFEPLPPVASPVKAPTPSGQSIPWDLWEPTESQAGVLPSGEPSSAEGSFAVAWPSQTAEPGPAQPAEASEVVGGTQEPGETAASEATSSSLPAVVVETFSATVNGAVEGSTTTGRLDLPPGFMFKVQAQHDYTATDTDELQLKAGDVVLVIPFQNPEEQDEGWLMGVKESDWNQHKELEKCRGVFPENFTERVQ amph_rat Amphiphysin Amph Amph1 protein protein rat Rattus norvegicus (Rat) Rat MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGMQEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSRKLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSRRVGFYVNTFKNVSSLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFSIQGAPSDSGPLRIAKTPSPPEEASPLPSPTASPNHTLAPASPAPVRPRSPSQTRKGPPVPPLPKVTPTKELQQENIINFFEDNFVPEINVTTPSQNEVLEVKKEETLLDLDFDPFKPDVTPAGSAAATHSPMSQTLPWDLWTTSTDLVQPASGGSFNDFTQPQDTSLFTMQTDQNMAETEQALPTEPQAEEPPTTAAAPTAGLDLGLEMEEPKEEAAIPPGTDAGETVGTEGSTGEEAEAEKAALPAGEGESPEGAKIDVESTELASSESPQAAELEAGAPQEKVIPSVVIEPASNHEGEEHQETTTGTETREATEDVAPQGPAGEKQELATEPTPLDSQAATPAPAGAVDASLSAGDAAQELPPGFLYKVETLHDFEAANSDELTLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRHLE necp1_mouse Adaptin ear-binding coat-associated protein 1 Necap1 NECAP endocytosis-associated protein 1 protein protein mouse Mus musculus Mouse MAAELEYESVLCVKPDVSVYRIPPRASNRGYRASDWKLDQPDWTGRLRITSKGKIAYIKLEDKVSGELFAQAPVEQYPGIAVETVTDSSRYFVIRIQDGTGRSAFIGIGFTDRGDAFDFNVSLQDHFKWVKQETEISKESQEMDNRPKLDLGFKEGQTIKLSIGNITAKKGGASKPRASGTGGLSLLPPPPGGKVTIPPPSSSVAISNHVTPPPIPKSNHGGNDSDILLDLDSPAPVSTSAPAPVSTSNDLWGDFSTASSSVPNQAPQPSNWVQF mint Involved in endocytosis ap2b1_human AP-2 complex subunit beta AP2B1 CLAPB1 Beta-2-adaptin Beta-adaptin Plasma membrane adaptor HA2/AP2 adaptin beta subunit Clathrin assembly protein complex 2 beta large chain AP105B Adaptor protein complex AP-2 subunit beta ADTB2 Adaptor-related protein complex 2 subunit beta protein protein human Homo sapiens Human MTDSKYFTTNKKGEIFELKAELNNEKKEKRKEAVKKVIAAMTVGKDVSSLFPDVVNCMQTDNLELKKLVYLYLMNYAKSQPDMAIMAVNSFVKDCEDPNPLIRALAVRTMGCIRVDKITEYLCEPLRKCLKDEDPYVRKTAAVCVAKLHDINAQMVEDQGFLDSLRDLIADSNPMVVANAVAALSEISESHPNSNLLDLNPQNINKLLTALNECTEWGQIFILDCLSNYNPKDDREAQSICERVTPRLSHANSAVVLSAVKVLMKFLELLPKDSDYYNMLLKKLAPPLVTLLSGEPEVQYVALRNINLIVQKRPEILKQEIKVFFVKYNDPIYVKLEKLDIMIRLASQANIAQVLAELKEYATEVDVDFVRKAVRAIGRCAIKVEQSAERCVSTLLDLIQTKVNYVVQEAIVVIRDIFRKYPNKYESIIATLCENLDSLDEPDARAAMIWIVGEYAERIDNADELLESFLEGFHDESTQVQLTLLTAIVKLFLKKPSETQELVQQVLSLATQDSDNPDLRDRGYIYWRLLSTDPVTAKEVVLSEKPLISEETDLIEPTLLDELICHIGSLASVYHKPPNAFVEGSHGIHRKHLPIHHGSTDAGDSPVGTTTATNLEQPQVIPSQGDLLGDLLNLDLGPPVNVPQVSSMQMGAVDLLGGGLDSLVGQSFIPSSVPATFAPSPTPAVVSSGLNDLFELSTGIGMAPGGYVAPKAVWLPAVKAKGLEISGTFTHRQGHIYMEMNFTNKALQHMTDFAIQFNKNSFGVIPSTPLAIHTPLMPNQSIDVSLPLNTLGPVMKMEPLNNLQVAVKNNIDVFYFSCLIPLNVLFVEDGKMERQVFLATWKDIPNENELQFQIKECHLNADTVSSKLQNNNVYTIAKRNVEGQDMLYQSLKLTNGIWILAELRIQPGNPNYTLSLKCRAPEVSQYIYQVYDSILKN necp1_rat Adaptin ear-binding coat-associated protein 1 Necap1 NECAP endocytosis-associated protein 1 protein protein rat Rattus norvegicus (Rat) Rat MAAELEYESVLCVKPDVSVYRIPPRASNRGYRASDWKLDQPDWTGRLRITSKGKIAYIKLEDKVSGELFAQAPVEQYPGIAVETVADSSRYFVIRIQDGTGRSAFIGIGFTDRGDAFDFNVSLQDHFKWVKQETEISKESQEMDSRPKLDLGFKEGQTIKLSIGNITAKKGGTSKPRASGTGGLSLLPPPPGGKVTIPPPSSSVAISNHVTPPPIPKSNHGSNDSDILLDLDSPAPVPTSAPAPAPASTSNDLWGDFSTASSSVPNQAPQPSNWVQF mint Involved in endocytosis fcho1_mouse F-BAR domain only protein 1 Fcho1 protein protein mouse Mus musculus Mouse MSYFGEHFWGDKNHGFEVLYHCVKQGPVATKELADFIRERANIEETYSKAMAKLSKLASNGTPMGTFAPLWEVFRVSSDKLALCHLELTRKLHDLLKDVLRYGEEQLKTHKKCKEEVLGTVDAVQMLSGVGQLLPKSRENYLSRCMDLERLRRENTSQKEMDKAETKSKKAADSLRRSVDKYNSARADFEIKMLDSALRFQAMEEAHLQHMKALLGSYAHSVEDTHVQIGQVHEEFKQNVENVTVDMLLRKFAESKGTGREKPGPLDFDAYSSAALQEAMKRLRGAKAFRLPGLSRREPRASVDFLESDSGVPPEVDDEGFTVRPDISQNNGAEPPRFSSSDSDFDDEEPRKFYVHIKPAPTRAVACSSEAAAAQLRATAGSLILPPGPGGTMKRHSSRDTSGKPQRPRSAPRTGSCAEKPLASEEPLSKSLFGPPLESAFDHDDFTGSSSLGFTSSPSPFSSSSPENVEDSGLDSPSHAAPGPSPESWVPRPGTPQSPPTCRAQHPEPRGLMPRAPSPGPWGPEGGADSLTPADPTREGLAATLRRPRSRKVSCPLTRSNGDLCRSLSPSPLGSSAPTIPPDRPSFSTQMGHGISRGPSPVVLGSQDALPVATAFTEYVHAYFRGHSPSCLARVTGELTMTFPAGIVRVFSGTPPPPVLSFRLVNTAPVEHFQPNADLIFSDPSQSDPETKDFWLNMAALTEALQHQAEQNPTASYYNLVLLRYQFSRPGPESVPLQMSAHWQCGPTLTRVSVEYSYRAGATAVSTPLTNVQILLPVGEPVTSVRLQPAATWNTEEKRFTWKLPDVCEAGGSGHLSASWQPQSGPSTPSPVAAQFTSEGATLSGLDLELLGGGYRMSLVKRRFATGMYLVSC q0jrz9-1 F-BAR domain only protein 2 FCHO2 protein protein human Homo sapiens Human MVMAYFVENFWGEKNSGFDVLYHNMKHGQISTKELADFVRERATIEEAYSRSMTKLAKSASNYSQLGTFAPVWDVFKTSTEKLANCHLDLVRKLQELIKEVQKYGEEQVKSHKKTKEEVAGTLEAVQTIQSITQALQKSKENYNAKCVEQERLKKEGATQREIEKAAVKSKKATDTYKLYVEKYALAKADFEQKMTETAQKFQDIEETHLIHIKEIIGSLSNAIKEIHLQIGQVHEEFINNMANTTVESLIQKFAESKGTGKERPGLIEFEECDTASAVEGIKPRKRKTFALPGIIKKEKDAESVECPDADSLNIPDVDEEGYSIKPETNQNDTKENHFYSSSDSDSEDEEPKKYRIEIKPMHPNNSHHTMASLDELKVSIGNITLSPAISRHSPVQMNRNLSNEELTKSKPSAPPNEKGTSDLLAWDPLFGPSLDSSSSSSLTSSSSARPTTPLSVGTIVPPPRPASRPKLTSGKLSGINEIPRPFSPPVTSNTSPPPAAPLARAESSSSISSSASLSAANTPTVGVSRGPSPVSLGNQDTLPVAVALTESVNAYFKGADPTKCIVKITGDMTMSFPSGIIKVFTSNPTPAVLCFRVKNISRLEQILPNAQLVFSDPSQCDSNTKDFWMNMQAVTVYLKKLSEQNPAASYYNVDVLKYQVSSNGIQSTPLNLATYWKCSASTTDLRVDYKYNPEAMVAPSVLSNIQVVVPVDGGVTNMQSLPPAIWNAEQMKAFWKLSSISEKSENGGSGSLRAKFDLSEGPSKPTTLAVQFLSEGSTLSGVDFELVGTGYRLSLIKKRFATGRYLADC ap2a2-amph-4 3259451 N/A 3259459 unspecified role unspecified role bait bait Region 695-938 Region 695-938 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3259460 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat N/A 3259461 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat association association false false false necap1-amph-10 3259452 N/A 3259462 unspecified role unspecified role bait bait Region 1-133 Region 1-133 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Q9CR95:p.Arg95Glu R95E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q9CR95:p.Arg102Ala R102A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Q9CR95:p.Arg102Lys R102K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3259460 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat N/A 3259461 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat association association false false false necap1-ap2b1-1 3259453 N/A 3259462 unspecified role unspecified role bait bait Region 1-133 Region 1-133 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3259463 unspecified role unspecified role prey prey Region 1-640 Region 1-640 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Region 592-937 Region 592-937 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human physical association physical association false false false ap2b1-necap1-1 3259454 N/A 3259464 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat N/A 3259463 unspecified role unspecified role bait bait Region 592-668 Region 592-668 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false necap1-fcho1-1 3259455 N/A 3259462 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-133 Region 1-133 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3259465 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-610 Region 1-610 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human physical association physical association false false false ap2b1-necap1-2 3259456 N/A 3259462 unspecified role unspecified role unspecified role unspecified role Region 1-133 Region 1-133 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3259463 unspecified role unspecified role unspecified role unspecified role Region 622-641 Region 622-641 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro direct interaction direct interaction false false false ap2a2-necap1-1 3259457 N/A 3259462 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3259459 unspecified role unspecified role bait bait Region 695-938 Region 695-938 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false necap1-fcho1-2 3259458 N/A 3259462 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-133 Region 1-133 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Q9CR95:p.Arg95Ala R95A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3259466 unspecified role unspecified role prey prey mCherry-tag mCherry-tag mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3259465 unspecified role unspecified role prey prey mCherry-tag mCherry-tag mcherry mcherry fluorescent protein tag mcherry n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human association association false false false