DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ wong-2013-1 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. DIP original experiment: DIP-260019X PLoS biology 2013 IMEx A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. Only protein-protein interactions imex curation wong-2013-2 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. DIP original experiment: DIP-260023X PLoS biology 2013 IMEx A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. Only protein-protein interactions imex curation wong-2013-3 drome Drosophila melanogaster (Fruit fly) Fruit fly anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. DIP original experiment: DIP-260021X PLoS biology 2013 IMEx A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. Only protein-protein interactions imex curation wong-2013-4 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. DIP original experiment: DIP-260025X PLoS biology 2013 IMEx A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. Only protein-protein interactions imex curation wong-2013-5 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. DIP original experiment: DIP-260020X PLoS biology 2013 IMEx A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. Only protein-protein interactions imex curation wong-2013-6 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. DIP original experiment: DIP-260024X PLoS biology 2013 IMEx A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. Only protein-protein interactions imex curation wong-2013-7 drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. DIP original experiment: DIP-260022X PLoS biology 2013 IMEx A Cullin1-based SCF E3 ubiquitin ligase targets the InR/PI3K/TOR pathway to regulate neuronal pruning. Wong JJ., Li S., Lim EK., Wang Y., Wang C., Zhang H., Kirilly D., Wu C., Liou YC., Wang H., Yu F. Only protein-protein interactions imex curation o77430_drome SkpA Dredd EG:115C2.4 skpA skpA-RC skpA-RH Dmel\CG16983 SKP1 SKPA Skp Skp1 anon-WO03040301.234 anon-WO03040301.236 anon-WO03040301.238 dSKP1 dSkp-1 dSkpA dskpA l(1)G0037 l(1)G0058 l(1)G0109 l(1)G0389 spkA Dmel_CG16983 CG16983 dSkip-1 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPSIKLQSSDEEIFDTDIQIAKCSGTIKTMLEDCGMEDDENAIVPLPNVNSTILRKVLTWAHYHKDDPQPTEDDESKEKRTDDIISWDADFLKVDQGTLFELILAANYLDIKGLLELTCKTVANMIKGKTPEEIRKTFNIKKDFSPAEEEQVRKENEWCEEK cul1_drome Cullin homolog 1 Cul1 Lin-19 homolog protein cul-1 lin19 CG1877 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNRSGNSQTTQKLVNLDDIWSELVEGIMQVFEHEKSLTRSQYMRFYTHVYDYCTSVSAAPSGRSSGKTGGAQLVGKKLYDRLEQFLKSYLSELLTKFKAISGEEVLLSRYTKQWKSYQFSSTVLDGICNYLNRNWVKRECEEGQKGIYKIYRLALVAWKGHLFQVLNEPVTKAVLKSIEEERQGKLINRSLVRDVIECYVELSFNEEDTDAEQQKLSVYKQNFENKFIADTSAFYEKESDAFLSTNTVTEYLKHVENRLEEETQRVRGFNSKNGLSYLHETTADVLKSTCEEVLIEKHLKIFHTEFQNLLNADRNDDLKRMYSLVALSSKNLTDLKSILENHILHQGTEAIAKCCTTDAANDPKTYVQTILDVHKKYNALVLTAFNNDNGFVAALDKACGKFINSNVVTIANSASKSPELLAKYCDLLLKKSSKNPEDKELEDNLNQVMVVFKYIEDKDVFQKYYSKMLAKRLVNHTSASDDAEAMMISKLKQTCGYEYTVKLQRMFQDIGVSKDLNSYFKQYLAEKNLTMEIDFGIEVLSSGSWPFQLSNNFLLPSELERSVRQFNEFYAARHSGRKLNWLYQMCKGELIMNVNRNNSSTYTLQASTFQMSVLLQFNDQLSFTVQQLQDNTQTQQENLIQVLQILLKAKVLTSSDNENSLTPESTVELFLDYKNKKRRININQPLKTELKVEQETVHKHIEEDRKLLIQAAIVRIMKMRKRLNHTNLISEVLNQLSTRFKPKVPVIKKCIDILIEKEYLERMEGHKDTYSYLA q9vde3_drome slmb beta-TrCP BcDNA:GM02031 Beta-TRCP1 Dmel\CG3412 FBXW1 MENE (3R)-B MENE(3R)-B SLIMB SLMB Slimb Slmb beta-TRCP betaTrCP crd ica l(3)00295 shv slimb wel Dmel_CG3412 CG3412 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MMKMETDKIMDETNSNAQAFTTTMLYDPVRKKDSSPTYQTERELCFQYFTQWSESGQVDFVEHLLSRMCHYQHGQINAYLKPMLQRDFITLLPIKGLDHIAENILSYLDAESLKSSELVCKEWLRVISEGMLWKKLIERKVRTDSLWRGLAERRNWMQYLFKPRPGQTQRPHSFHRELFPKIMNDIDSIENNWRTGRHMLRRINCRSENSKGVYCLQYDDGKIVSGLRDNTIKIWDRTDLQCVKTLMGHTGSVLCLQYDDKVIISGSSDSTVRVWDVNTGEMVNTLIHHCEAVLHLRFNNGMMVTCSKDRSIAVWDMTSPSEITLRRVLVGHRAAVNVVDFDEKYIVSASGDRTIKVWSTSSCEFVRTLNGHKRGIACLQYRDRLVVSGSSDNSIRLWDIECGACLRVLEGHEELVRCIRFDTKRIVSGAYDGKIKVWDLVAALDPRAASNTLCLNTLVEHTGRVFRLQFDEFQIVSSSHDDTILIWDFLNFTPNENKTGRTPSPALMEH akt1_drome RAC serine/threonine-protein kinase Akt1 CG4006 Akt Protein kinase B protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNYLPFVLQRRSTVVASAPAPGSASRIPESPTTTGSNIINIIYSQSTHPNSSPTSGSAEKFSWQQSWPSRTSAAPTHDSGTMSINTTFDLSSPSVTSGHALTEQTQVVKEGWLMKRGEHIKNWRQRYFVLHSDGRLMGYRSKPADSASTPSDFLLNNFTVRGCQIMTVDRPKPFTFIIRGLQWTTVIERTFAVESELERQQWTEAIRNVSSRLIDVGEVAMTPSEQTDMTDVDMATIAEDELSEQFSVQGTTCNSSGVKKVTLENFEFLKVLGKGTFGKVILCREKATAKLYAIKILKKEVIIQKDEVAHTLTESRVLKSTNHPFLISLKYSFQTNDRLCFVMQYVNGGELFWHLSHERIFTEDRTRFYGAEIISALGYLHSQGIIYRDLKLENLLLDKDGHIKVADFGLCKEDITYGRTTKTFCGTPEYLAPEVLDDNDYGQAVDWWGTGVVMYEMICGRLPFYNRDHDVLFTLILVEEVKFPRNITDEAKNLLAGLLAKDPKKRLGGGKDDVKEIQAHPFFASINWTDLVLKKIPPPFKPQVTSDTDTRYFDKEFTGESVELTPPDPTGPLGSIAEEPLFPQFSYQGDMASTLGTSSHISTSTSLASMQ ubiq_drome Ubiquitin RpL40 CG2960 RpS27A UBI-F80 Ubi-f CG5271 Ubi-p63E Ubi-m CG11624 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG rbx1a_drome RING-box protein 1A Roc1a CG16982 Regulator of cullins 1a dRbx1 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MEVDEDGYEVPSSSSKGDKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWDFQKYGH slmb-cul1-1 3258852 N/A 3258859 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258860 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258861 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly association association false false false slmb-akt1-1 3258853 N/A 3258862 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258861 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 196-510 Region 196-510 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false akt1-slmb-1 3258854 N/A 3258862 unspecified role unspecified role bait bait drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258861 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false akt1-ub-2 3258855 N/A 3258863 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258862 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false slmb-cul1-2 3258856 N/A 3258864 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258859 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258860 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258861 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly association association false false false akt1-slmb-2 3258857 N/A 3258863 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258862 unspecified role unspecified role bait bait Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258861 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly association association false false false slmb-akt1-2 3258858 N/A 3258862 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly N/A 3258861 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association false false false