DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ back-2013-1 ecolx Escherichia coli nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. DIP original experiment: DIP-255829X Structure (London, England : 1993) 2013 IMEx High-resolution structural analysis shows how Tah1 tethers Hsp90 to the R2TP complex. Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. Only protein-protein interactions imex curation back-2013-2 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. DIP original experiment: DIP-255827X Structure (London, England : 1993) 2013 IMEx High-resolution structural analysis shows how Tah1 tethers Hsp90 to the R2TP complex. Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. Only protein-protein interactions imex curation back-2013-3 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. DIP original experiment: DIP-255832X Structure (London, England : 1993) 2013 IMEx High-resolution structural analysis shows how Tah1 tethers Hsp90 to the R2TP complex. Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. Only protein-protein interactions imex curation back-2013-4 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. DIP original experiment: DIP-255828X Structure (London, England : 1993) 2013 IMEx High-resolution structural analysis shows how Tah1 tethers Hsp90 to the R2TP complex. Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. Only protein-protein interactions imex curation back-2013-5 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. DIP original experiment: DIP-255830X Structure (London, England : 1993) 2013 IMEx High-resolution structural analysis shows how Tah1 tethers Hsp90 to the R2TP complex. Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. Only protein-protein interactions imex curation back-2013-6 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. DIP original experiment: DIP-255834X Structure (London, England : 1993) 2013 IMEx High-resolution structural analysis shows how Tah1 tethers Hsp90 to the R2TP complex. Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. Only protein-protein interactions imex curation back-2013-7 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. DIP original experiment: DIP-255833X Structure (London, England : 1993) 2013 IMEx High-resolution structural analysis shows how Tah1 tethers Hsp90 to the R2TP complex. Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. Only protein-protein interactions imex curation back-2013-8 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. DIP original experiment: DIP-255835X Structure (London, England : 1993) 2013 IMEx High-resolution structural analysis shows how Tah1 tethers Hsp90 to the R2TP complex. Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. Only protein-protein interactions imex curation back-2013-9 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. DIP original experiment: DIP-255836X Structure (London, England : 1993) 2013 IMEx High-resolution structural analysis shows how Tah1 tethers Hsp90 to the R2TP complex. Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. Only protein-protein interactions imex curation back-2013-10 ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. DIP original experiment: DIP-255831X Structure (London, England : 1993) 2013 IMEx High-resolution structural analysis shows how Tah1 tethers Hsp90 to the R2TP complex. Back R., Dominguez C., Rothé B., Bobo C., Beaufils C., Moréra S., Meyer P., Charpentier B., Branlant C., Allain FH., Manival X. Only protein-protein interactions imex curation pih1_yeast Protein interacting with Hsp90 1 PIH1 NOP17 Nucleolar protein 17 YHR034C protein protein yeast Saccharomyces cerevisiae Baker's yeast MADFLLRPIKQRHRNEDKYVSVDAADGSVSKIEPIADFVIKTKLLSANGPEKLQDGRKVFINVCHSPLVPKPEVDFNARIVFPLIIQNEWEIPIITSCYRMDHDKKGQECYVWDCCINSDCSRWICDDIQLREILVEWCLESCEIRDSVVLCRDRIAFPKMKKKGAELPALEVLNDELHQDYKAKMHKIIEEEAGDPMSILRGRNDDGDDNNDPDDGTLPPLFPIENKISGAKIEEIDKNEIAHRNLKQAPAPAPAPHEQQEDVPEYEVKMKRFKGAAYKLRILIENKAPNSKPDRFSPSYNFAENILYINGKLSIPLPRDIVVNAADIKIFHIRKERTLYIYI 02C06D0A113B1B3B tah1_yeast TPR repeat-containing protein associated with Hsp90 YCR60W YCR060W TAH1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQFEKQKEQGNSLFKQGLYREAVHCYDQLITAQPQNPVGYSNKAMALIKLGEYTQAIQMCQQGLRYTSTAEHVAIRSKLQYRLELAQGAVGSVQIPVVEVDELPEGYDRS 3738DE6F227F803A hsp82_yeast ATP-dependent molecular chaperone HSP82 HSP90 YPL240C HSP82 Heat shock protein Hsp90 heat-inducible isoform 82 kDa heat shock protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MASETFEFQAEITQLMSLIINTVYSNKEIFLRELISNASDALDKIRYKSLSDPKQLETEPDLFIRITPKPEQKVLEIRDSGIGMTKAELINNLGTIAKSGTKAFMEALSAGADVSMIGQFGVGFYSLFLVADRVQVISKSNDDEQYIWESNAGGSFTVTLDEVNERIGRGTILRLFLKDDQLEYLEEKRIKEVIKRHSEFVAYPIQLVVTKEVEKEVPIPEEEKKDEEKKDEEKKDEDDKKPKLEEVDEEEEKKPKTKKVKEEVQEIEELNKTKPLWTRNPSDITQEEYNAFYKSISNDWEDPLYVKHFSVEGQLEFRAILFIPKRAPFDLFESKKKKNNIKLYVRRVFITDEAEDLIPEWLSFVKGVVDSEDLPLNLSREMLQQNKIMKVIRKNIVKKLIEAFNEIAEDSEQFEKFYSAFSKNIKLGVHEDTQNRAALAKLLRYNSTKSVDELTSLTDYVTRMPEHQKNIYYITGESLKAVEKSPFLDALKAKNFEVLFLTDPIDEYAFTQLKEFEGKTLVDITKDFELEETDEEKAEREKEIKEYEPLTKALKEILGDQVEKVVVSYKLLDAPAAIRTGQFGWSANMERIMKAQALRDSSMSSYMSSKKTFEISPKSPIIKELKKRVDEGGAQDKTVKDLTKLLYETALLTSGFSLDEPTSFASRINRLISLGLNIDEDEETETAPEASTAAPVEEVPADTEMEEVD D7C35676D668FB63 pih1-tah1-1 3257793 N/A 3257803 unspecified role unspecified role unspecified role unspecified role Region 257-344 Region 257-344 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3257804 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false hsp90-tah1-1 3257794 N/A 3257805 unspecified role unspecified role unspecified role unspecified role Region 701-709 Region 701-709 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3257804 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false tah1-pih1-19 3257795 N/A 3257803 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3257804 unspecified role unspecified role bait bait Region 93-111 Region 93-111 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false hsp90-tah1-2 3257796 N/A 3257805 unspecified role unspecified role unspecified role unspecified role Region 701-709 Region 701-709 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3257804 unspecified role unspecified role unspecified role unspecified role P25638:p.Lys79Ala K79A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P25638:p.Tyr82Ala Y82A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false pih1-tah1-2 3257797 N/A 3257803 unspecified role unspecified role unspecified role unspecified role Region 257-344 Region 257-344 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3257804 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false pih1-tah1-3 3257798 N/A 3257803 unspecified role unspecified role bait bait Region 253-344 Region 253-344 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3257804 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false tah1-hsp90-1 3257799 N/A 3257805 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3257804 unspecified role unspecified role bait bait P25638:p.Lys79Ala K79A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P25638:p.Tyr82Ala Y82A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false Region 1-92 Region 1-92 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false P25638:p.Arg83Ala R83A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false tah1-hsp90-2 3257800 N/A 3257805 unspecified role unspecified role prey prey P02829:p.Asp709Ala D709A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P02829:p.Glu707Ala E707A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P02829:p.Glu706Ala E706A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P02829:p.Val708Ala V708A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3257804 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false pih1-hsp90-1 3257801 N/A 3257803 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast N/A 3257805 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association false false false tah1-pih1-20 3257802 N/A 3257803 unspecified role unspecified role prey prey Region 257-344 Region 257-344 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3257804 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli physical association physical association false false false