DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ wiener-2013-1 in vitro In vitro deubiquitinase assay deubiquitinase assay deubiquitinase assay weight by comassie molecular weight estimation by coomasie staining weight by comassie Wiener R., DiBello AT., Lombardi PM., Guzzo CM., Zhang X., Matunis MJ., Wolberger C. DIP original experiment: DIP-255644X Nature structural & molecular biology 2013 IMEx E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Wiener R., DiBello AT., Lombardi PM., Guzzo CM., Zhang X., Matunis MJ., Wolberger C. Only protein-protein interactions imex curation wiener-2013-2 in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Wiener R., DiBello AT., Lombardi PM., Guzzo CM., Zhang X., Matunis MJ., Wolberger C. DIP original experiment: DIP-255645X Nature structural & molecular biology 2013 IMEx E2 ubiquitin-conjugating enzymes regulate the deubiquitinating activity of OTUB1. Wiener R., DiBello AT., Lombardi PM., Guzzo CM., Zhang X., Matunis MJ., Wolberger C. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG q96fw1-1 Ubiquitin thioesterase OTUB1 OTUB1 OTB1 OTU1 Deubiquitinating enzyme OTUB1 OTU domain-containing ubiquitin aldehyde-binding protein 1 Otubain-1 Ubiquitin-specific-processing protease OTUB1 HSPC263 Otubain-1 protein protein human Homo sapiens Human MAAEEPQQQKQEPLGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPKVYLLYRPGHYDILYK otub1-ub-3 3257108 N/A 3257110 enzyme target enzyme target substrate substrate unspecified role unspecified role K48-Ub K48-Ub ubiquitinylated lysine ubiquitinylated lysine certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3257111 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli deubiquitination deubiquitination reaction deubiquitination false false false otub1-ub-4 3257109 N/A 3257110 enzyme target enzyme target substrate substrate unspecified role unspecified role AMC-label AMC-label amc label aminomethylcoumarin label AMC label c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal false ecolx Escherichia coli N/A 3257111 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli protein cleavage protein cleavage false false false