DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ hatzivassiliou-2013-1 in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. DIP original experiment: DIP-254602X Nature 2013 IMEx Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. Only protein-protein interactions imex curation hatzivassiliou-2013-2 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. DIP original experiment: DIP-254597X Nature 2013 IMEx Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. Only protein-protein interactions imex curation hatzivassiliou-2013-3 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. DIP original experiment: DIP-254600X Nature 2013 IMEx Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. Only protein-protein interactions imex curation hatzivassiliou-2013-4 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. DIP original experiment: DIP-254598X Nature 2013 IMEx Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. Only protein-protein interactions imex curation hatzivassiliou-2013-5 in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. DIP original experiment: DIP-254601X Nature 2013 IMEx Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. Only protein-protein interactions imex curation hatzivassiliou-2013-6 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. DIP original experiment: DIP-254599X Nature 2013 IMEx Mechanism of MEK inhibition determines efficacy in mutant KRAS- versus BRAF-driven cancers. Hatzivassiliou G., Haling JR., Chen H., Song K., Price S., Heald R., Hewitt JF., Zak M., Peck A., Orr C., Merchant M., Hoeflich KP., Chan J., Luoh SM., Anderson DJ., Ludlam MJ., Wiesmann C., Ultsch M., Friedman LS., Malek S., Belvin M. Only protein-protein interactions imex curation braf_human Serine/threonine-protein kinase B-raf BRAF BRAF1 RAFB1 v-Raf murine sarcoma viral oncogene homolog B1 p94 Proto-oncogene B-Raf protein protein human Homo sapiens Human MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH mp2k1_human Dual specificity mitogen-activated protein kinase kinase 1 ERK activator kinase 1 MAPK/ERK kinase 1 MAP2K1 MEK1 PRKMK1 protein protein human Homo sapiens Human MPKKKPTPIQLNPAPDGSAVNGTSSAETNLEALQKKLEELELDEQQRKRLEAFLTQKQKVGELKDDDFEKISELGAGNGGVVFKVSHKPSGLVMARKLIHLEIKPAIRNQIIRELQVLHECNSPYIVGFYGAFYSDGEISICMEHMDGGSLDQVLKKAGRIPEQILGKVSIAVIKGLTYLREKHKIMHRDVKPSNILVNSRGEIKLCDFGVSGQLIDSMANSFVGTRSYMSPERLQGTHYSVQSDIWSMGLSLVEMAVGRYPIPPPDAKELELMFGCQVEGDAAETPPRPRTPGRPLSSYGMDSRPPMAIFELLDYIVNEPPPKLPSGVFSLEFQDFVNKCLIKNPAERADLKQLMVHAFIKRSDAEEVDFAGWLCSTIGLNQPSTPTHAAGV raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF map2k1-braf-28 3256659 N/A 3256665 unspecified role unspecified role prey prey Kinase domain Region 416-766 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3256666 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction false false false map2k1-braf-29 3256660 N/A 3256665 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3256667 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3256666 unspecified role unspecified role bait bait human Homo sapiens Human association association false false false raf1-braf-22 3256661 N/A 3256665 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3256667 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false map2k1-braf-30 3256662 N/A 3256665 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3256666 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false map2k1-raf1-1 3256663 N/A 3256667 unspecified role unspecified role prey prey Kinase domain Region 306-648 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm N/A 3256666 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction false false false braf-raf1-31 3256664 N/A 3256665 unspecified role unspecified role bait bait human Homo sapiens Human N/A 3256667 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false