DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ xu-2013-18 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Xu J., Shen C., Wang T., Quan J. DIP original experiment: DIP-255630X Nature structural & molecular biology 2013 IMEx Structural basis for the inhibition of Polo-like kinase 1. Xu J., Shen C., Wang T., Quan J. Only protein-protein interactions imex curation xu-2013-19 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Xu J., Shen C., Wang T., Quan J. DIP original experiment: DIP-255628X Nature structural & molecular biology 2013 IMEx Structural basis for the inhibition of Polo-like kinase 1. Xu J., Shen C., Wang T., Quan J. Only protein-protein interactions imex curation xu-2013-20 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Xu J., Shen C., Wang T., Quan J. DIP original experiment: DIP-255629X Nature structural & molecular biology 2013 IMEx Structural basis for the inhibition of Polo-like kinase 1. Xu J., Shen C., Wang T., Quan J. Only protein-protein interactions imex curation q4kmi8_danre Serine/threonine-protein kinase PLK plk1 Polo-like kinase protein protein danre Danio rerio (Zebrafish) Brachydanio rerio Zebrafish MSAAIAKPSAKPSAHVDPKSAPLKEIPDVLVDPRTMKRYMRGRFLGKGGFAKCYEITDMDTKEVFAGKVVPKSMLLKPHQKEKMSTEIAIHKSLDNPHVVGFHGFFEDDDFVYVVLEICRRRSLLELHKRRKAVTEPEARYFMRQTIQGVQYLHNNRVIHRDLKLGNLFLNDDMDVKIGDFGLATKIEFDGERKKTLCGTPNYIAPEVLCKKGHSFEVDIWSLGCILYTLLVGKPPFETSCLKETYIRIKKNEYSVPRHINPVASALIRRMLHADPTLRPSVAELLTDEFFTSGYAPMRLPTSCLTVPPRFSIAPSSLDPALLRKPLSSLNKGTDSPIEEMGKMDQPQREDLQQRDGPEQPDTHLTDMLQQLAVVNAAKPSDRDFIRQEEAEDPACIPVFWISKWVDYSDKYGLGYQLSDNSVGVLFNDSTRLIMCADGDSLQYIDRNSLESYLSVRSYPSALSKKITLLKYFRNYMSEHLLKAGANITPREGDELTRLPYLRHWFRTKSAIVLHLSNGTVQINFFQDHTKLILCPLMGAVTYINEKREFYTYKMTLIEEFGCCKELASRLRYARNMVEKLMACKSSTTAATSAR ma205_drome 205 kDa microtubule-associated protein Map205 CG1483 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MEHHEDNAQLDNYLQNRLAESLQICGGAGEHNPHLADATGGNGCAPGIAPSKSDEVDGEEDEEWKYIHEVRQSEKLQQEKLPLTKETGNGFGPGRDSDNQVHGNGAAAVFNLYEEDVEVIKNDGDFSTNSNTTTSTDEVVARQAQEPNQLPEQLQQQQQIESQGVHEDPRQEDEDEHSSVATTYGTSSLSENNSSPLDQEEVVMVAQTVGQEQLVDFDNKENSYFVKNLEENHSQLNPNAVAFVPGVGSQSSSPLPAAEDPLPGVQPRPFLPGGTLDDLVAESPRKEFARINMDGIAVPDEREFDIEADMRPHELEQESDTFGAGHLEMQLLNGIGTADQAALRDVLDHGPETSVDMELPLDQVPNDADIMKQSIYAEHNSSIEDILNSVQPLPIQTCDDKELIHVEEKEHVSKSPSTEELQFQSDFPNNQESHTLFNNTEQDPMQASFYLEHTSQKAQEGCQEQMQLPAECSDIFADQSLLLDTSAPQLSSEADSPVAKLELESQQAGIVDITPSPLSSTAEKHLVEDTKELVEEYTLDPESHFFGVVSSQAPLQLFGKHTLPSIIHSCKHRVASEQNDEENAVFESVSGYETQNFDEISSPPEGINPFAQPFTPAHLVIEQANTMMEDVGGMPIPASEDFAICDKVASKSSNEVEDHRSEQQAFVKEELLHPVGDVVAQVENLGTEKNFVVEEERLPISVSDEIPLSSASKEKLLPDTTDEQLLTSALEEKLRSVAPEESVSTAADGQSISQFDEYVIASNKPLEDILEPEKDVEVAKSLSEKTSVATVAGGAVVGATKTHSATKAGSTAASAKSKTETLVMKKTTASSTSVYGANKSAAPRPSTARLGIKSTSIATKTSTTSSLTGNPRKSLSSNVGSTVKPPTKLSGTRPATAPVSKVTLGAKTITNKPTASGTASDNVTRTTLRPLVSTNARRPATSGTGSVASSTARRPVTNAKGSAPGSAASTKVRPAATMTAPVKPKVLSPRSTISSTTTVRKVPSTSTPSFSTRSPNKQQSNGLGKNTSSTTTSTATATITKSFTARSAPKFTHSASLTYNNGSTSRRLLVPGSSSTTTTSSLRKSSPLKAAPGKAASKPLTPQSKDGTAKSSPAVLKARNSTLMGGEGVAPSNECVPTPNGQINAEEVVKLKGKGLAEEVKIIEEQKLHEQDVPAHNAEVPLLDF plk1-plk1-1 3256220 N/A 3256223 unspecified role unspecified role bait bait Region 359-595 Region 359-595 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3256223 unspecified role unspecified role prey prey Region 17-312 Region 17-312 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Q4KMI8:p.Ser123Asp S123D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q4KMI8:p.Arg130Glu R130E mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q4KMI8:p.Thr306Lys T306K mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Q4KMI8:p.Val307Arg V307R mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli putative self interaction putative self interaction false false false map205-plk1-1 3256221 N/A 3256224 unspecified role unspecified role unspecified role unspecified role Region 276-325 Region 276-325 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3256223 unspecified role unspecified role unspecified role unspecified role Region 17-312/360-595 Region 17-312/360-595 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false map205-plk1-2 3256222 N/A 3256224 unspecified role unspecified role unspecified role unspecified role Region 276-325 Region 276-325 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3256223 unspecified role unspecified role unspecified role unspecified role Region 17-312/360-595 Region 17-312/360-595 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false