DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ chang-2013-6 in vitro In vitro comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Chang TK., Shravage BV., Hayes SD., Powers CM., Simin RT., Wade Harper J., Baehrecke EH. DIP original experiment: DIP-266949X Nature cell biology 2013 IMEx Uba1 functions in Atg7- and Atg3-independent autophagy. Chang TK., Shravage BV., Hayes SD., Powers CM., Simin RT., Wade Harper J., Baehrecke EH. Only protein-protein interactions imex curation chang-2013-7 in vitro In vitro comigration in sds comigration in sds page comigration in sds anti tag western anti tag western blot anti tag western Chang TK., Shravage BV., Hayes SD., Powers CM., Simin RT., Wade Harper J., Baehrecke EH. DIP original experiment: DIP-266950X Nature cell biology 2013 IMEx Uba1 functions in Atg7- and Atg3-independent autophagy. Chang TK., Shravage BV., Hayes SD., Powers CM., Simin RT., Wade Harper J., Baehrecke EH. Only protein-protein interactions imex curation ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG q8t0l3_drome Ubiquitin-activating enzyme E1 Uba1 2 31 DmUba1 Dmel\CG1782 E1 FBtr0088499 UBA-1 Uba 1 l(2)03405 l(2)05642 l(2)s3484 uba-1 uba1 CG1782 Dmel_CG1782 UBA1 Uba protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSSGQQLMADSPACSSSDHSPLAKKRRLELLGDGVGAMGMEEEEAGGATSSSSSNNYENHDKAGGVDISGKCQSKTTSEHQLELCVSSSTSDTCGVNAAAKRAEKQKLKQSQGSGDDVSTSSAVEKEGSKRPEKRNYLNTTTIESQEASSSASATSNSNNNVSSSNNNSNNSTNNRQLEGSSNMAGNSAAAGGDIDESLYSRQLYVLGHDAMRRMANSDILLSGLGGLGLEIAKNVILGGVKSITLHDTATCGLHDLSSQFYLTEADIGKNRAEASCAQLAELNNYVRTVSHTGPLTEEFLRKFRVVVLTNSDGEEQQRIAKFAHENGIALIIAETRGLFAKVFCDFGESFTIYDQDGTQPISTMIASITHDAQGVVTCLDETRHGFNDGDYVTFSEVQGMQELNGCQPLKITVLGPYTFSIGDTSKFGEYKSGGVATQVKMPKTISFKPLAQATEEPEFLISDFAKLDSPATLHVAFNALSCYRKAHNGALPRPWNEEDANSFLEVVRASSNAEVDEKLVLQFAKICSGNTCPLDAAVGGIVAQEVLKACSGKFTPIYQWLYFDALECLPTEGVEEADAQPVGSRYDSQIAIFGKKFQEKLADSKWFIVGAGAIGCELLKNFGMLGLGTGNGQIFVTDMDLIEKSNLNRQFLFRPHDVQKPKSMTAADAIKRMNPEVNVTAYELRVGAETEKVFSEDFFGKLDGVANALDNVDARIYMDRKCIFNRIPLVETGTLGTLGNVQVIVPFATESYSSSQDPPEKSIPICTLKNFPNAIEHTLQWARDAFEGVFKQSAENAAQYIADPQFTERIAKLPGIQPLEILDSIKKALIDDKPKSFAHCVEWARLYWEDQYVNQIKQLLFNFPPDQITSSGQPFWSGPKRCPDPLVFDVNDPMHLDFIYAAANLRAEVYGIEQVRNRETIAELVQKVKVPEFKPRSGVKIETNEAAAAASANNFDDGELDQDRVDKIISELLKNADKSSKITPLEFEKDDDSNLHMDFIVACSNLRAANYKIPPADRHKSKLIAGKIIPAIATTTSVLSGLAVLEVIKLIVGHRDLVKFKNGFANLALPFMAFSEPLPAAKNTYYGKEWTLWDRFEVTGELSLQEFLNYFEENEKLKITMLSQGVSMLYSFFMPKAKCSERLPLPMSEVVRRVSKRRLEPHERSLVFEICCNDVDGEDVEVPYVRYTLP q9w2s2_drome Atg-8a Atg8alpha Atg8a CG1534 ATG8 ATG8a Atg8 Atg8A BcDNA:LD05816 DmAtg8a Dmel\CG32672 DrAtg8a LC3 atg8 atg8A atg8a dAtg8a CG32672 Dmel_CG32672 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MKFQYKEEHAFEKRRAEGDKIRRKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNVIPPTSATMGSLYQEHHEEDYFLYIAYSDENVYGMAKIN q7jy94_drome Ubiquitin-like modifier-activating enzyme ATG7 Atg7 ATG7 Dmel\CG5489 atg-7 atg7 CG5489 Dmel_CG5489 dAtg7 Autophagy-related protein 7 ATG-7 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSTEKEIILQFAPWESFVSPTFWHKLAELKLDHDRLSDSKRSITGHYTNRNASGCLLEVDYTAYNRMAKPPKFSHSAIGTIYNKNTIEEFKALDKLQLLADEGKELLADMCSGGALRDPSLLTRFFVLSFADLKCHSYYYWFAFPCPLTPTLKLQGAVQKLRDLPNSSSYIMALKALPTESQNFFILYANVEKNIFEARSLSSLDDKNVEFCYFGFADPSEYEHPAWIMRNYAAFLLQQCPSFVGKPLKFLGLRHNQQMNIDDSLVWKVIQTEACDLSQSENIKFVGWELNKNGKMGPRMVCMRDSMDPAKLAENSVNLNLKLMKWRLVPDLNLEIISQTKCLLFGAGTLGCAVARNLLSWGFKHITLLDSGKVGFSNPVRQNLYTHADAVAGNRMKATTAAQRLKEINPSAETAGYVLEIPMPGHTIGESLLAQTKEHLKVIEKLVQDHDVIFLLTDSRESRWLPTLLGAAKEKIVINAALGFDSYLVMRHGTTRKEAGDDGQEIEGLKCINGDQLGCYFCNDVTAPGNSLKDRTLDQQCTVTRPGVSNIAASYAVELLVALLQHPRKELAPAYYAQSGRGRSEETEEKVPEGLLGILPHSIRGMLCNYENILPATQKFAQCIACSAAVLNEYKKEGHAFLFKTFETAKFLEDLTGISEFKRLNSEIIDFDDEEFDMSDSDED ub-uba1-5 3256084 N/A 3256086 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3256087 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false atg7-atg8a-1 3256085 N/A 3256088 unspecified role unspecified role unspecified role unspecified role His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3256089 unspecified role unspecified role unspecified role unspecified role Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction false false false