DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ zhang-2013-39 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Zhang S., Lu G., Qi J., Li Y., Zhang Z., Zhang B., Fan Z., Yan J., Gao GF. DIP original experiment: DIP-254644X Structure (London, England : 1993) 2013 IMEx Competition of cell adhesion and immune recognition: insights into the interaction between CRTAM and nectin-like 2. Zhang S., Lu G., Qi J., Li Y., Zhang Z., Zhang B., Fan Z., Yan J., Gao GF. Only protein-protein interactions imex curation zhang-2013-40 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhang S., Lu G., Qi J., Li Y., Zhang Z., Zhang B., Fan Z., Yan J., Gao GF. DIP original experiment: DIP-254642X Structure (London, England : 1993) 2013 IMEx Competition of cell adhesion and immune recognition: insights into the interaction between CRTAM and nectin-like 2. Zhang S., Lu G., Qi J., Li Y., Zhang Z., Zhang B., Fan Z., Yan J., Gao GF. Only protein-protein interactions imex curation zhang-2013-41 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Zhang S., Lu G., Qi J., Li Y., Zhang Z., Zhang B., Fan Z., Yan J., Gao GF. DIP original experiment: DIP-254648X Structure (London, England : 1993) 2013 IMEx Competition of cell adhesion and immune recognition: insights into the interaction between CRTAM and nectin-like 2. Zhang S., Lu G., Qi J., Li Y., Zhang Z., Zhang B., Fan Z., Yan J., Gao GF. Only protein-protein interactions imex curation zhang-2013-42 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhang S., Lu G., Qi J., Li Y., Zhang Z., Zhang B., Fan Z., Yan J., Gao GF. DIP original experiment: DIP-254646X Structure (London, England : 1993) 2013 IMEx Competition of cell adhesion and immune recognition: insights into the interaction between CRTAM and nectin-like 2. Zhang S., Lu G., Qi J., Li Y., Zhang Z., Zhang B., Fan Z., Yan J., Gao GF. Only protein-protein interactions imex curation zhang-2013-43 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhang S., Lu G., Qi J., Li Y., Zhang Z., Zhang B., Fan Z., Yan J., Gao GF. DIP original experiment: DIP-254647X Structure (London, England : 1993) 2013 IMEx Competition of cell adhesion and immune recognition: insights into the interaction between CRTAM and nectin-like 2. Zhang S., Lu G., Qi J., Li Y., Zhang Z., Zhang B., Fan Z., Yan J., Gao GF. Only protein-protein interactions imex curation zhang-2013-44 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Zhang S., Lu G., Qi J., Li Y., Zhang Z., Zhang B., Fan Z., Yan J., Gao GF. DIP original experiment: DIP-254643X Structure (London, England : 1993) 2013 IMEx Competition of cell adhesion and immune recognition: insights into the interaction between CRTAM and nectin-like 2. Zhang S., Lu G., Qi J., Li Y., Zhang Z., Zhang B., Fan Z., Yan J., Gao GF. Only protein-protein interactions imex curation zhang-2013-45 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Zhang S., Lu G., Qi J., Li Y., Zhang Z., Zhang B., Fan Z., Yan J., Gao GF. DIP original experiment: DIP-254645X Structure (London, England : 1993) 2013 IMEx Competition of cell adhesion and immune recognition: insights into the interaction between CRTAM and nectin-like 2. Zhang S., Lu G., Qi J., Li Y., Zhang Z., Zhang B., Fan Z., Yan J., Gao GF. Only protein-protein interactions imex curation cadm1_human Cell adhesion molecule 1 CADM1 IGSF4 IGSF4A NECL2 SYNCAM TSLC1 Immunoglobulin superfamily member 4 Nectin-like protein 2 Spermatogenic immunoglobulin superfamily Synaptic cell adhesion molecule Tumor suppressor in lung cancer 1 protein protein human Homo sapiens Human MASVVLPSGSQCAAAAAAAAPPGLRLRLLLLLFSAAALIPTGDGQNLFTKDVTVIEGEVATISCQVNKSDDSVIQLLNPNRQTIYFRDFRPLKDSRFQLLNFSSSELKVSLTNVSISDEGRYFCQLYTDPPQESYTTITVLVPPRNLMIDIQKDTAVEGEEIEVNCTAMASKPATTIRWFKGNTELKGKSEVEEWSDMYTVTSQLMLKVHKEDDGVPVICQVEHPAVTGNLQTQRYLEVQYKPQVHIQMTYPLQGLTREGDALELTCEAIGKPQPVMVTWVRVDDEMPQHAVLSGPNLFINNLNKTDNGTYRCEASNIVGKAHSDYMLYVYDPPTTIPPPTTTTTTTTTTTTTILTIITDSRAGEEGSIRAVDHAVIGGVVAVVVFAMLCLLIILGRYFARHKGTYFTHEAKGADDAADADTAIINAEGGQNNSEEKKEYFI CDEDE1E0C08BDD3A o95727-1 Cytotoxic and regulatory T-cell molecule CRTAM Class-I MHC-restricted T-cell-associated molecule protein protein human Homo sapiens Human MWWRVLSLLAWFPLQEASLTNHTETITVEEGQTLTLKCVTSLRKNSSLQWLTPSGFTIFLNEYPALKNSKYQLLHHSANQLSITVPNVTLQDEGVYKCLHYSDSVSTKEVKVIVLATPFKPILEASVIRKQNGEEHVVLMCSTMRSKPPPQITWLLGNSMEVSGGTLHEFETDGKKCNTTSTLIIHTYGKNSTVDCIIRHRGLQGRKLVAPFRFEDLVTDEETASDALERNSLSSQDPQQPTSTVSVTEDSSTSEIDKEEKEQTTQDPDLTTEANPQYLGLARKKSGILLLTLVSFLIFILFIIVQLFIMKLRKAHVIWKKENEVSEHTLESYRSRSNNEETSSEEKNGQSSHPMRCMNYITKLYSEAKTKRKENVQHSKLEEKHIQVPESIV cadm1-crtam-1 3256032 N/A 3256039 unspecified role unspecified role bait bait Region 45-144 Region 45-144 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3256040 unspecified role unspecified role prey prey Region 18-117 Region 18-117 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O95727-1:p.[Phe56Ala;Thr57Ala;Lys67Ala;Tyr101Ala] F56A/T57A/K67A/Y101A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false O95727-1:p.Phe56Ala F56A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O95727-1:p.Thr57Ala T57A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O95727-1:p.Lys67Ala K67A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O95727-1:p.Tyr101Ala Y101A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cadm1-crtam-2 3256033 N/A 3256039 unspecified role unspecified role unspecified role unspecified role Region 45-144 Region 45-144 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3256040 unspecified role unspecified role unspecified role unspecified role Region 18-117 Region 18-117 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false crtam-4 3256034 N/A 3256040 unspecified role unspecified role unspecified role unspecified role Region 18-117 Region 18-117 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false crtam-5 3256035 N/A 3256040 unspecified role unspecified role unspecified role unspecified role Region 18-117 Region 18-117 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cadm1-1 3256036 N/A 3256039 unspecified role unspecified role unspecified role unspecified role Region 45-144 Region 45-144 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cadm1-crtam-3 3256037 N/A 3256039 unspecified role unspecified role unspecified role unspecified role Region 45-144 Region 45-144 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3256040 unspecified role unspecified role unspecified role unspecified role Region 18-117 Region 18-117 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false cadm1-crtam-4 3256038 N/A 3256039 unspecified role unspecified role unspecified role unspecified role Region 45-144 Region 45-144 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3256040 unspecified role unspecified role unspecified role unspecified role Region 18-117 Region 18-117 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false