DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ jrgens-2013-1 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. DIP original experiment: DIP-266872X Nature chemical biology 2013 IMEx The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. Only protein-protein interactions imex curation jrgens-2013-2 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. DIP original experiment: DIP-266875X Nature chemical biology 2013 IMEx The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. Only protein-protein interactions imex curation jrgens-2013-3 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. DIP original experiment: DIP-266873X Nature chemical biology 2013 IMEx The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. Only protein-protein interactions imex curation jrgens-2013-4 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. DIP original experiment: DIP-266874X Nature chemical biology 2013 IMEx The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. Only protein-protein interactions imex curation jrgens-2013-5 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. DIP original experiment: DIP-266877X Nature chemical biology 2013 IMEx The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. Only protein-protein interactions imex curation jrgens-2013-6 in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. DIP original experiment: DIP-266876X Nature chemical biology 2013 IMEx The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry. Jürgens MC., Vörös J., Rautureau GJ., Shepherd DA., Pye VE., Muldoon J., Johnson CM., Ashcroft AE., Freund SM., Ferguson N. Only protein-protein interactions imex curation p03139-1 Large envelope protein S L glycoprotein L-HBsAg Large S protein Large surface protein Major surface antigen L-HBsAg LHB Large envelope protein protein protein hbvd1 Hepatitis B virus genotype D subtype adw (isolate United Kingdom/adyw/1979) HBV-D MGQNLSTSNPLGFFPDHQLDPAFRANTNNPDWDFNPNKDTWPDANKVGAGAFGLGFTPPHGGLLGWSPQAQGIMQTLPANPPPASTNRQSGRQPTPLSPPLRTTHPQAMHWNSTTFHQTLQDPRVRGLYFPAGGSSSGTVNPVPTTTSPISSIFSRIGDPALNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGTTVCLGQNSQSPISNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCPLIPGSSTTSTGSCRTCTTPAQGISMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLWEWASARFSWLSLLVPFVQWFVGLSPIVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCLWAYI ap1g2_human AP-1 complex subunit gamma-like 2 AP1G2 Gamma2-adaptin protein protein human Homo sapiens Human MVVPSLKLQDLIEEIRGAKTQAQEREVIQKECAHIRASFRDGDPVHRHRQLAKLLYVHMLGYPAHFGQMECLKLIASSRFTDKRVGYLGAMLLLDERHDAHLLITNSIKNDLSQGIQPVQGLALCTLSTMGSAEMCRDLAPEVEKLLLQPSPYVRKKAILTAVHMIRKVPELSSVFLPPCAQLLHERHHGILLGTITLITELCERSPAALRHFRKVVPQLVHILRTLVTMGYSTEHSISGVSDPFLQVQILRLLRILGRNHEESSETMNDLLAQVATNTDTSRNAGNAVLFETVLTIMDIRSAAGLRVLAVNILGRFLLNSDRNIRYVALTSLLRLVQSDHSAVQRHRPTVVECLRETDASLSRRALELSLALVNSSNVRAMMQELQAFLESCPPDLRADCASGILLAAERFAPTKRWHIDTILHVLTTAGTHVRDDAVANLTQLIGGAQELHAYSVRRLYNALAEDISQQPLVQVAAWCIGEYGDLLLAGNCEEIEPLQVDEEEVLALLEKVLQSHMSLPATRGYALTALMKLSTRLCGDNNRIRQVVSIYGSCLDVELQQRAVEYDTLFRKYDHMRAAILEKMPLVERDGPQADEEAKESKEAAQLSEAAPVPTEPQASQLLDLLDLLDGASGDVQHPPHLDPSPGGALVHLLDLPCVPPPPAPIPDLKVFEREGVQLNLSFIRPPENPALLLITITATNFSEGDVTHFICQAAVPKSLQLQLQAPSGNTVPARGGLPITQLFRILNPNKAPLRLKLRLTYDHFHQSVQEIFEVNNLPVESWQ ap1g2-s-1 3255832 N/A 3255838 unspecified role unspecified role unspecified role unspecified role Region 1-108 Region 1-108 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3255839 unspecified role unspecified role unspecified role unspecified role Region 665-785 Region 665-785 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ap1g2-s-2 3255833 N/A 3255838 unspecified role unspecified role unspecified role unspecified role Region 9-16 Region 9-16 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 29-36 Region 29-36 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 59-68 Region 59-68 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3255839 unspecified role unspecified role unspecified role unspecified role Region 665-785 Region 665-785 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ap1g2-s-3 3255834 N/A 3255838 unspecified role unspecified role unspecified role unspecified role Region 1-108 Region 1-108 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3255839 unspecified role unspecified role unspecified role unspecified role Region 665-785 Region 665-785 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false O75843:p.Ala716Gly A716G mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false O75843:p.Lys719Gln K719Q mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false O75843:p.Ser720Ala S720A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O75843:p.Pro754Ala P754A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O75843:p.Arg756Ala R756A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false O75843:p.Arg756Gln R756Q mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false O75843:p.Arg760Ala R760A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ap1g2-s-4 3255835 N/A 3255838 unspecified role unspecified role unspecified role unspecified role Region 42-108 Region 42-108 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false P03139-1:p.Asp31Ala D31A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P03139-1:p.Trp32Ala W32A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P03139-1:p.Asp33Ala D33A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false P03139-1:p.Phe34Ala F34A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3255839 unspecified role unspecified role unspecified role unspecified role Region 665-785 Region 665-785 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ap1g2-s-5 3255836 N/A 3255838 unspecified role unspecified role unspecified role unspecified role Region 29-36 Region 29-36 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3255839 unspecified role unspecified role unspecified role unspecified role Region 665-785 Region 665-785 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false ap1g2-s-6 3255837 N/A 3255838 unspecified role unspecified role unspecified role unspecified role Region 9-16 Region 9-16 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 29-36 Region 29-36 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Region 59-68 Region 59-68 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false in vitro In vitro N/A 3255839 unspecified role unspecified role unspecified role unspecified role Region 665-785 Region 665-785 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false