DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ pan-2013-7 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254740X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Pan D., Nakatsu T., Kato H. Only protein-protein interactions imex curation pan-2013-8 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254745X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Pan D., Nakatsu T., Kato H. Only protein-protein interactions imex curation pan-2013-9 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254747X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Pan D., Nakatsu T., Kato H. Only protein-protein interactions imex curation pan-2013-10 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254744X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Pan D., Nakatsu T., Kato H. Only protein-protein interactions imex curation pan-2013-11 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254742X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Pan D., Nakatsu T., Kato H. Only protein-protein interactions imex curation pan-2013-12 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254746X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Pan D., Nakatsu T., Kato H. Only protein-protein interactions imex curation pan-2013-13 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254741X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Pan D., Nakatsu T., Kato H. Only protein-protein interactions imex curation pan-2013-14 in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Pan D., Nakatsu T., Kato H. DIP original experiment: DIP-254743X Nature structural & molecular biology 2013 IMEx Crystal structure of peroxisomal targeting signal-2 bound to its receptor complex Pex7p-Pex21p. Pan D., Nakatsu T., Kato H. Only protein-protein interactions imex curation thik_yeast 3-ketoacyl-CoA thiolase, peroxisomal POT1 FOX3 POX3 YIL160C Beta-ketothiolase Acetyl-CoA acyltransferase Peroxisomal 3-oxoacyl-CoA thiolase protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQRLQSIKDHLVESAMGKGESKRKNSLLEKRPEDVVIVAANRSAIGKGFKGAFKDVNTDYLLYNFLNEFIGRFPEPLRADLNLIEEVACGNVLNVGAGATEHRAACLASGIPYSTPFVALNRQCSSGLTAVNDIANKIKVGQIDIGLALGVESMTNNYKNVNPLGMISSEELQKNREAKKCLIPMGITNENVAANFKISRKDQDEFAANSYQKAYKAKNEGLFEDEILPIKLPDGSICQSDEGPRPNVTAESLSSIRPAFIKDRGTTTAGNASQVSDGVAGVLLARRSVANQLNLPVLGRYIDFQTVGVPPEIMGVGPAYAIPKVLEATGLQVQDIDIFEINEAFAAQALYCIHKLGIDLNKVNPRGGAIALGHPLGCTGARQVATILRELKKDQIGVVSMCIGTGMGAAAIFIKE B22AA06130829DE5 pex21_yeast Peroxisomal membrane protein PEX21 YGR239C G8593 Peroxin-21 PEX21 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPSVCHTSPIEKIIQQGHRIQNDSLIPSKRTKLAHTELTAHYATEDSHVEKHFLHNGSNFDGIDNVRYQNQPSPLTFITPNNTVDSSDWVPQFSSMKIDDSLEFSSEYKRLYSNYESQQRLNSSRQHLPFKNCMIRKTSCTYPPQKTLRQQRQGNRDNPTDAFQFDAEFQVLEREIQKERYEPITRRDEKWFDQDQSELQRIATDIVKCCTPPPSSASSSSTLSSSVESKLSESKFIQLMRNISSGDVTLKKNADGNSASELFSSNNGELVGNRHIFVKDEIHKDILD 0197257317ADE4FF pex7_yeast Peroxisomal targeting signal 2 receptor PEX7 PAS7 PEB1 YDR142C YD2943.01C YD9302.18C Peroxin-7 Peroxisome import protein PAS7 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLRYHMQGFSGYGVQYSPFFDNRLAVAAGSNFGLVGNGKLFILEIDRSGRIVEVNSFLTQDCLFDLAWNESHENQVLVAQGDGTLRLFDTTFKEFPIAIFKEHEREVFSCNWNLVNRQNFLSSSWDGSIKIWSPLRKQSLMTLTPRPLEITKMVDPLNAIILKKKSFTGISKNRNCVYQAQFSPHDQNLVLSCSGNSYASLFDIRLPSGKNQNNFLVHSGLEALTCDFNKYRPYVVATGGVDNAIRIWDIRMLNKNESATIKRTVPGQLHNSSCINEIPNAHGLAIRKVTWSPHHSNILMSASYDMTCRIWRDLSNDGAKETYKTNSTDATKGSIFNFTQHSEFVFGADWSLWGKPGYVASTAWDGNLFVWNGLG 7D53A42A8ACF23F9 fox3p-pex21p-1 3255312 N/A 3255320 unspecified role unspecified role unspecified role unspecified role Region 1-15 Region 1-15 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3255321 unspecified role unspecified role unspecified role unspecified role Region 190-288 Region 190-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3255322 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false pex21p-pex7p-1 3255313 N/A 3255321 unspecified role unspecified role bait bait Region 190-288 Region 190-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3255322 unspecified role unspecified role prey prey picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false fox3p-pex21p-2 3255314 N/A 3255320 unspecified role unspecified role unspecified role unspecified role Region 1-15 Region 1-15 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false ecolx Escherichia coli N/A 3255321 unspecified role unspecified role unspecified role unspecified role Region 190-288 Region 190-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3255322 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris physical association physical association false false false pex21p-fox3p-1 3255315 N/A 3255320 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3255321 unspecified role unspecified role bait bait Region 190-288 Region 190-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false P50091:p.Phe236Asp F236D mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3255322 unspecified role unspecified role prey prey picpa Pichia pastoris (Yeast) Yeast Pichia pastoris association association false false false pex7p-pex21p-1 3255316 N/A 3255321 unspecified role unspecified role prey prey Region 190-288 Region 190-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3255322 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false pex21p-fox3p-2 3255317 N/A 3255320 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3255321 unspecified role unspecified role bait bait Region 190-288 Region 190-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3255322 unspecified role unspecified role prey prey P39108:p.Leu34Asp L34D mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false P39108:p.[Phe32Ala;Leu34Ala] F32A/L34A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false P39108:p.[Phe344Ala;Trp364Ala] F344A/W364A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain false certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris association association false false false pex7p-fox3p-1 3255318 N/A 3255320 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3255321 unspecified role unspecified role prey prey Region 190-288 Region 190-288 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3255322 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris association association false false false pex7p-fox3p-2 3255319 N/A 3255320 unspecified role unspecified role prey prey ecolx Escherichia coli N/A 3255322 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris direct interaction direct interaction false false false