DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ whorton-2013-1 trini Trichoplusia ni (Cabbage looper) Cabbage looper ion exchange chrom ion exchange chromatography IEC IEC ion exchange chrom predetermined predetermined participant predetermined Whorton MR., MacKinnon R. DIP original experiment: DIP-252850X Nature 2013 IMEx X-ray structure of the mammalian GIRK2-βγ G-protein complex. Whorton MR., MacKinnon R. Only protein-protein interactions imex curation whorton-2013-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Whorton MR., MacKinnon R. DIP original experiment: DIP-252848X Nature 2013 IMEx X-ray structure of the mammalian GIRK2-βγ G-protein complex. Whorton MR., MacKinnon R. Only protein-protein interactions imex curation whorton-2013-3 trini Trichoplusia ni (Cabbage looper) Cabbage looper molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Whorton MR., MacKinnon R. DIP original experiment: DIP-252851X Nature 2013 IMEx X-ray structure of the mammalian GIRK2-βγ G-protein complex. Whorton MR., MacKinnon R. Only protein-protein interactions imex curation whorton-2013-4 trini Trichoplusia ni (Cabbage looper) Cabbage looper pull down pull down predetermined predetermined participant predetermined Whorton MR., MacKinnon R. DIP original experiment: DIP-252849X Nature 2013 IMEx X-ray structure of the mammalian GIRK2-βγ G-protein complex. Whorton MR., MacKinnon R. Only protein-protein interactions imex curation gbg2_human Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 G gamma-I GNG2 protein protein human Homo sapiens Human MASNNTASIAQARKLVEQLKMEANIDRIKVSKAAADLMAYCEAHAKEDPLLTPVPASENPFREKKFFCAIL gbb1_human Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 GNB1 Transducin beta chain 1 protein protein human Homo sapiens Human MSELDQLRQEAEQLKNQIRDARKACADATLSQITNNIDPVGRIQMRTRRTLRGHLAKIYAMHWGTDSRLLVSASQDGKLIIWDSYTTNKVHAIPLRSSWVMTCAYAPSGNYVACGGLDNICSIYNLKTREGNVRVSRELAGHTGYLSCCRFLDDNQIVTSSGDTTCALWDIETGQQTTTFTGHTGDVMSLSLAPDTRLFVSGACDASAKLWDVREGMCRQTFTGHESDINAICFFPNGNAFATGSDDATCRLFDLRADQELMTYSHDNIICGITSVSFSKSGRLLLAGYDDFNCNVWDALKADRAGVLAGHDNRVSCLGVTDDGMAVATGSWDSFLKIWN kcnj6_mouse G protein-activated inward rectifier potassium channel 2 Kcnj6 Girk2 Kcnj7 W Inward rectifier K(+) channel Kir3.2 Potassium channel, inwardly rectifying subfamily J member 6 protein protein mouse Mus musculus Mouse MTMAKLTESMTNVLEGDSMDQDVESPVAIHQPKLPKQARDDLPRHISRDRTKRKIQRYVRKDGKCNVHHGNVRETYRYLTDIFTTLVDLKWRFNLLIFVMVYTVTWLFFGMIWWLIAYIRGDMDHIEDPSWTPCVTNLNGFVSAFLFSIETETTIGYGYRVITDKCPEGIILLLIQSVLGSIVNAFMVGCMFVKISQPKKRAETLVFSTHAVISMRDGKLCLMFRVGDLRNSHIVEASIRAKLIKSKQTSEGEFIPLNQSDINVGYYTGDDRLFLVSPLIISHEINQQSPFWEISKAQLPKEELEIVVILEGIVEATGMTCQARSSYITSEILWGYRFTPVLTMEDGFYEVDYNSFHETYETSTPSLSAKELAELANRAEVPLSWSVSSKLNQHAELETEEEEKNPEELTERNGDVANLENESKV gnb1-gng2-6 3254619 N/A 3254623 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3254624 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false gnb1-gng2-7 3254620 N/A 3254623 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3254624 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3254625 unspecified role unspecified role unspecified role unspecified role Region 52-380 Region 52-380 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false picpa Pichia pastoris (Yeast) Yeast Pichia pastoris physical association physical association false false false gnb1-gng2-8 3254621 N/A 3254623 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3254624 unspecified role unspecified role unspecified role unspecified role trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false gng2-gnb1-2 3254622 N/A 3254623 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper N/A 3254624 unspecified role unspecified role prey prey trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association false false false