DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ winkler-2013-1 ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. DIP original experiment: DIP-254716X Nature structural & molecular biology 2013 IMEx A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression. Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. Only protein-protein interactions imex curation winkler-2013-2 in vitro In vitro mst microscale thermophoresis mst predetermined predetermined participant predetermined Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. DIP original experiment: DIP-254722X Nature structural & molecular biology 2013 IMEx A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression. Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. Only protein-protein interactions imex curation winkler-2013-3 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. DIP original experiment: DIP-254720X Nature structural & molecular biology 2013 IMEx A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression. Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. Only protein-protein interactions imex curation winkler-2013-4 ecolx Escherichia coli light scattering light scattering predetermined predetermined participant predetermined Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. DIP original experiment: DIP-254717X Nature structural & molecular biology 2013 IMEx A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression. Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. Only protein-protein interactions imex curation winkler-2013-5 in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. DIP original experiment: DIP-254723X Nature structural & molecular biology 2013 IMEx A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression. Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. Only protein-protein interactions imex curation winkler-2013-6 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. DIP original experiment: DIP-254725X Nature structural & molecular biology 2013 IMEx A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression. Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. Only protein-protein interactions imex curation winkler-2013-7 in vitro In vitro sls static light scattering sls predetermined predetermined participant predetermined Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. DIP original experiment: DIP-254721X Nature structural & molecular biology 2013 IMEx A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression. Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. Only protein-protein interactions imex curation winkler-2013-8 ecolx Escherichia coli mst microscale thermophoresis mst predetermined predetermined participant predetermined Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. DIP original experiment: DIP-254718X Nature structural & molecular biology 2013 IMEx A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression. Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. Only protein-protein interactions imex curation winkler-2013-9 in vitro In vitro h1-h2 ms mass spectrometry study of hydrogen/deuterium exchange h1-h2 ms predetermined predetermined participant predetermined Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. DIP original experiment: DIP-254724X Nature structural & molecular biology 2013 IMEx A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression. Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. Only protein-protein interactions imex curation winkler-2013-10 ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. DIP original experiment: DIP-254719X Nature structural & molecular biology 2013 IMEx A ternary AppA-PpsR-DNA complex mediates light regulation of photosynthesis-related gene expression. Winkler A., Heintz U., Lindner R., Reinstein J., Shoeman RL., Schlichting I. Only protein-protein interactions imex curation q3j179_cers4 ppsR RSP_0282 protein protein rhos4 Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) MLAGGSLPSLAPDLVRDLIATAADISLLVSQEGVVREVMANPHHPSFGQLSEWEGRPLEEVLTAESVAKFRLRSEGLEPGRGSVAVELNHIDPRSFEFPIRYILHRLPADRSILMLGRDLRPIAEVQQQLVAAQLAMERDYETQREMETRYRVVLDVSRDPMVLVSMSTGRIVDLNSAAGLLLGGVRQDLLGAAIAQEFEGRRRGEFMETMTNLAATESAAPVEVLARRSQKRLLVVPRVFRAAGERLLLCQIDPADATQPVGDELSENLARLYHEGVDGIVFSDADGTIRGANEAFLNMTDSSSLAAIRGRSIADFLARGSVDLRVLIDSVRRTGQLRLYATRLTTDFAGQIAAEISATWLDDRERPLLVLVVRDTSRADTMRRPVPATGVIDEPARNVMELVGNSTLKDIVAETTDVVEKMCIETALELTRNNRVAAAEMLSLSRQSLYVKLRKFGLLNKDE q3j677_cers4 appA RSP_1565 protein protein rhos4 Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158) MQHDLEADVTMTGSDLVSCCYRSLAAPDLTLRDLLDIVETSQAHNARAQLTGALFYSQGVFFQWLEGRPAAVAEVMTHIQRDRRHSNVEILAEEPIAKRRFAGWHMQLSCSEADMRSLGLAESRQIVTVGRSLVADNTNIFSFDRIAAVRRFLSDVCAARTLAPDTPVEADTFALYALTEAQAGRSGRAKAVARLSDLLSTDPLGRLTEVEELLRAHAPTAADFARLFEACAERLTRALAEDRISRMQVTLAYSALQMALRRIHHLPDPQKSVGAVLVAGVPGHKPILEAALAAEMLRAVGWSTSVVHPESVAALAARLKTSRTSTLVVAPSLLEGTEQEADTLRFVSALRARTDLPGLSILVGGRLAQLPPSKLKDSGADAGFAHLALLPAALARVACPANADCCSMRACRMPASQCCDKRINPEFLLANVMPSVLTRISSRQDRRRSA ppsr-1 3254573 N/A 3254583 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false appa-ppsr-1 3254574 N/A 3254584 unspecified role unspecified role unspecified role unspecified role Region 1-399 Region 1-399 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3254583 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false appa-ppsr-2 3254575 N/A 3254584 unspecified role unspecified role unspecified role unspecified role Region 1-399 Region 1-399 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3254583 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ppsr-2 3254576 N/A 3254583 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false appa-ppsr-3 3254577 N/A 3254584 unspecified role unspecified role unspecified role unspecified role Region 1-399 Region 1-399 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3254583 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false appa-ppsr-4 3254578 N/A 3254584 unspecified role unspecified role unspecified role unspecified role Region 168-399 Region 168-399 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3254583 unspecified role unspecified role unspecified role unspecified role Region 1-257 Region 1-257 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false appa-ppsr-5 3254579 N/A 3254584 unspecified role unspecified role unspecified role unspecified role Region 1-399 Region 1-399 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3254583 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ppsr-3 3254580 N/A 3254583 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false appa-ppsr-6 3254581 N/A 3254584 unspecified role unspecified role unspecified role unspecified role Region 1-399 Region 1-399 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3254583 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false ppsr-4 3254582 N/A 3254583 unspecified role unspecified role unspecified role unspecified role Region 1-379 Region 1-379 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false