DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ pan-2013-4 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Pan Z., Zhu J., Shang Y., Wei Z., Jia M., Xia C., Wen W., Wang W., Zhang M. DIP original experiment: DIP-252333X Structure (London, England : 1993) 2013 IMEx An autoinhibited conformation of LGN reveals a distinct interaction mode between GoLoco motifs and TPR motifs. Pan Z., Zhu J., Shang Y., Wei Z., Jia M., Xia C., Wen W., Wang W., Zhang M. Only protein-protein interactions imex curation pan-2013-5 in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Pan Z., Zhu J., Shang Y., Wei Z., Jia M., Xia C., Wen W., Wang W., Zhang M. DIP original experiment: DIP-252335X Structure (London, England : 1993) 2013 IMEx An autoinhibited conformation of LGN reveals a distinct interaction mode between GoLoco motifs and TPR motifs. Pan Z., Zhu J., Shang Y., Wei Z., Jia M., Xia C., Wen W., Wang W., Zhang M. Only protein-protein interactions imex curation pan-2013-6 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Pan Z., Zhu J., Shang Y., Wei Z., Jia M., Xia C., Wen W., Wang W., Zhang M. DIP original experiment: DIP-252334X Structure (London, England : 1993) 2013 IMEx An autoinhibited conformation of LGN reveals a distinct interaction mode between GoLoco motifs and TPR motifs. Pan Z., Zhu J., Shang Y., Wei Z., Jia M., Xia C., Wen W., Wang W., Zhang M. Only protein-protein interactions imex curation gpsm2_mouse G-protein-signaling modulator 2 Gpsm2 Lgn Pins Pins homolog protein protein mouse Mus musculus Mouse MEGNLISMREDHSFHVRYRMEASCLELALEGERLCKSGDCRAGVSFFEAAVQVGTEDLKTLSAIYSQLGNAYFYLHDYAKALEYHHHDLTLARTIGDQLGEAKASGNLGNTLKVLGNFDEAIVCCQRHLDISRELNDKVGEARALYNLGNVYHAKGKSFGCPGPQDTGEFPEDVRNALQAAVDLYEENLSLVTALGDRAAQGRAFGNLGNTHYLLGNFRDAVIAHEQRLLIAKEFGDKAAERRAYSNLGNAYIFLGEFETASEYYKKTLLLARQLKDRAVEAQSCYSLGNTYTLLQDYEKAIDYHLKHLAIAQELKDRIGEGRACWSLGNAYTALGNHDQAMHFAEKHLEISREVGDKSGELTARLNLSDLQMVLGLSYSTNNSMMSENIEIDGSLHGAGAKLGRRHSMENLELMKLTPEKVPNWNSEILAKQKPLIAKPSAKLLFVNRLKGKKYKSGSACTKVLQDASNSVDHRAPRSQKKISSDTIGDEGFFDLLRRFQSNRMDDQRCHLQGNCRTTSTAAASATPKLMKAPSVSVVSPNTDEFLDLLASSQSRRLDDQRASFSNLPGLRLTKGNSPSVLERLMTNDKKEPDEDFFDILVKCQGSRLDDQRCAPPSAATKGPTVPDEDFFSLILRSQAKRMDEQRVLLQRDPNRDSEFGLKELLQNNALLEFKHSGK mint homomint Plays an important role in spindle pole orientation (By similarity). Interacts and contributes to the functional activity of G(i) alpha proteins. Acts to stabilize the apical complex during neuroblast divisions q14980-2 Nuclear mitotic apparatus protein 1 Nuclear mitotic apparatus protein NUMA1 NMP22 NUMA Nuclear matrix protein-22 SP-H antigen protein protein human Homo sapiens Human MTLHATRGAALLSWVNSLHVADPVEAVLQLQDCSIFIKIIDRIHGTEEGQQILKQPVSERLDFVCSFLQKNRKHPSSPECLVSAQKVLEGSELELAKMTMLLLYHSTMSSKSPRDWEQFEYKIQAELAVILKFVLDHEDGLNLNEDLENFLQKAPVPSTCSSTFPEELSPPSHQAKREIRFLELQKVASSSSGNNFLSGSPASPMGDILQTPQFQMRRLKKQLADERSNRDELELELAENRKLLTEKDAQIAMMQQRIDRLALLNEKQAASPLEPKELEELRDKNESLTMRLHETLKQCQDLKTEKSQMDRKINQLSEENGDLSFKLREFASHLQQLQDALNELTEEHSKATQEWLEKQAQLEKELSAALQDKKCLEEKNEILQGKLSQLEEHLSQLQDNPPQEKGEVLGDVLQLETLKQEAATLAANNTQLQARVEMLETERGQQEAKLLAERGHFEEEKQQLSSLITDLQSSISNLSQAKEELEQASQAHGARLTAQVASLTSELTTLNATIQQQDQELAGLKQQAKEKQAQLAQTLQQQEQASQGLRHQVEQLSSSLKQKEQQLKEVAEKQEATRQDHAQQLATAAEEREASLRERDAALKQLEALEKEKAAKLEILQQQLQVANEARDSAQTSVTQAQREKAELSRKVEELQACVETARQEQHEAQAQVAELELQLRSEQQKATEKERVAQEKDQLQEQLQALKESLKVTKGSLEEEKRRAADALEEQQRCISELKAETRSLVEQHKRERKELEEERAGRKGLEARLQQLGEAHQAETEVLRRELAEAMAAQHTAESECEQLVKEVAAWRERYEDSQQEEAQYGAMFQEQLMTLKEECEKARQELQEAKEKVAGIESHSELQISRQQNELAELHANLARALQQVQEKEVRAQKLADDLSTLQEKMAATSKEVARLETLVRKAGEQQETASRELVKEPARAGDRQPEWLEEQQGRQFCSTQAALQAMEREAEQMGNELERLRAALMESQGQQQEERGQQEREVARLTQERGRAQADLALEKAARAELEMRLQNALNEQRVEFATLQEALAHALTEKEGKDQELAKLRGLEAAQIKELEELRQTVKQLKEQLAKKEKEHASGSGAQSEAAGRTEPTGPKLEALRAEVSKLEQQCQKQQEQADSLERSLEAERASRAERDSALETLQGQLEEKAQELGHSQSALASAQRELAAFRTKVQDHSKAEDEWKAQVARGRQEAERKNSLISSLEEEVSILNRQVLEKEGESKELKRLVMAESEKSQKLEERLRLLQAETASNSARAAERSSALREEVQSLREEAEKQRVASENLRQELTSQAERAEELGQELKAWQEKFFQKEQALSTLQLEHTSTQALVSELLPAKHLCQQLQAEQAAAEKRHREELEQSKQAAGGLRAELLRAQRELGELIPLRQKVAEQERTAQQLRAEKASYAEQLSMLKKAHGLLAEENRGLGERANLGRQFLEVELDQAREKYVQELAAVRADAETRLAEVQREAQSTARELEVMTAKYEGAKVKVLEERQRFQEERQKLTAQVEELSKKLADSDQASKVQQQKLKAVQAQGGESQQEAQRLQAQLNELQAQLSQKEQAAEHYKLQMEKAKTHYDAKKQQNQELQEQLRSLEQLQKENKELRAEAERLGHELQQAGLKTKEAEQTCRHLTAQVRSLEAQVAHADQQLRDLGKFQVATDALKSREPQAKPQLDLSIDSLDLSCEEGTPLSITSKLPRTQPDGTSVPGEPASPISQRLPPKVESLESLYFTPIPARSQAPLESSLDSLGDVFLDSGRKTRSARRRTTQIINITMTKKLDVEEPDSANSSFYSTRSAPASQASLRATSSTQSLARLGSPDYGNSALLSLPGYRPTTRSSARRSQAGVSSGAPPGRNSFYMGTCQDEPEQLDDWNRIAELQQRNRVCPPHLKTCYPLESRPSLSLGTITDEEMKTGDPQETLRRASMQPIQIAEGTGITTRQQRKRVSLEPHQGPGTPESKKATSCFPRPMTPRDRHEGRKQSTTEAQKKAAPASTKQADRRQSMAFSILNTPKKLGNSLLRRGASKKALSKASPNTRSGTRRSPRIATTTASAATAAAIGATPRAKGKAKH No experimental confirmation available. gnai3_mouse Guanine nucleotide-binding protein G(i) subunit alpha-3 G(i) alpha-3 Gnai3 protein protein mouse Mus musculus Mouse MGCTLSAEDKAAVERSKMIDRNLREDGEKAAKEVKLLLLGAGESGKSTIVKQMKIIHEDGYSEDECKQYKVVVYSNTIQSIIAIIRAMGRLKIDFGESARADDARQLFVLAGSAEEGVMTSELAGVIKRLWRDGGVQACFSRSREYQLNDSASYYLNDLDRISQTNYIPTQQDVLRTRVKTTGIVETHFTFKELYFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKRSPLTICYPEYTGSNTYEEAAAYIQCQFEDLNRRKDTKEVYTHFTCATDTKNVQFVFDAVTDVIIKNNLKECGLY gnai3-gpsm2-3 3254046 N/A 3254049 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3254050 unspecified role unspecified role unspecified role unspecified role Region 1886-1958 Region 1886-1958 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3254051 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association false false false gpsm2-numa1-2 3254047 N/A 3254049 unspecified role unspecified role unspecified role unspecified role Region 15-350 Region 15-350 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3254050 unspecified role unspecified role unspecified role unspecified role Region 1896-1912 Region 1896-1912 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false FITC-label FITC-label fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false gpsm2-numa1-6 3254048 N/A 3254049 unspecified role unspecified role unspecified role unspecified role Region 15-350 Region 15-350 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3254050 unspecified role unspecified role unspecified role unspecified role Region 1886-1958 Region 1886-1958 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false