DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ shen-2013-1 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. DIP original experiment: DIP-252878X Nature 2013 IMEx EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. Only protein-protein interactions imex curation shen-2013-2 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. DIP original experiment: DIP-252887X Nature 2013 IMEx EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. Only protein-protein interactions imex curation shen-2013-3 in vitro In vitro pull down pull down autoradiography autoradiography Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. DIP original experiment: DIP-252882X Nature 2013 IMEx EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. Only protein-protein interactions imex curation shen-2013-4 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. DIP original experiment: DIP-252890X Nature 2013 IMEx EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. Only protein-protein interactions imex curation shen-2013-5 human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. DIP original experiment: DIP-252879X Nature 2013 IMEx EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. Only protein-protein interactions imex curation shen-2013-6 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. DIP original experiment: DIP-252891X Nature 2013 IMEx EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. Only protein-protein interactions imex curation shen-2013-7 human Homo sapiens Human bifc bimolecular fluorescence complementation bifc predetermined predetermined participant predetermined Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. DIP original experiment: DIP-252883X Nature 2013 IMEx EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. Only protein-protein interactions imex curation shen-2013-8 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. DIP original experiment: DIP-252888X Nature 2013 IMEx EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. Only protein-protein interactions imex curation shen-2013-9 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. DIP original experiment: DIP-252880X Nature 2013 IMEx EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. Only protein-protein interactions imex curation shen-2013-10 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. DIP original experiment: DIP-252884X Nature 2013 IMEx EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. Only protein-protein interactions imex curation shen-2013-11 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. DIP original experiment: DIP-252885X Nature 2013 IMEx EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. Only protein-protein interactions imex curation shen-2013-12 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. DIP original experiment: DIP-252881X Nature 2013 IMEx EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. Only protein-protein interactions imex curation shen-2013-13 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. DIP original experiment: DIP-252886X Nature 2013 IMEx EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. Only protein-protein interactions imex curation shen-2013-14 human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. DIP original experiment: DIP-252889X Nature 2013 IMEx EGFR modulates microRNA maturation in response to hypoxia through phosphorylation of AGO2. Shen J., Xia W., Khotskaya YB., Huo L., Nakanishi K., Lim SO., Du Y., Wang Y., Chang WC., Chen CH., Hsu JL., Wu Y., Lam YC., James BP., Liu X., Liu CG., Patel DJ., Hung MC. Only protein-protein interactions imex curation ago2_human Protein argonaute-2 AGO2 Argonaute RISC catalytic component 2 EIF2C2 Eukaryotic translation initiation factor 2C 2 PAZ Piwi domain protein Protein slicer protein protein human Homo sapiens Human MYSGAGPALAPPAPPPPIQGYAFKPPPRPDFGTSGRTIKLQANFFEMDIPKIDIYHYELDIKPEKCPRRVNREIVEHMVQHFKTQIFGDRKPVFDGRKNLYTAMPLPIGRDKVELEVTLPGEGKDRIFKVSIKWVSCVSLQALHDALSGRLPSVPFETIQALDVVMRHLPSMRYTPVGRSFFTASEGCSNPLGGGREVWFGFHQSVRPSLWKMMLNIDVSATAFYKAQPVIEFVCEVLDFKSIEEQQKPLTDSQRVKFTKEIKGLKVEITHCGQMKRKYRVCNVTRRPASHQTFPLQQESGQTVECTVAQYFKDRHKLVLRYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIRATARSAPDRQEEISKLMRSASFNTDPYVREFGIMVKDEMTDVTGRVLQPPSILYGGRNKAIATPVQGVWDMRNKQFHTGIEIKVWAIACFAPQRQCTEVHLKSFTEQLRKISRDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTTPQTLSNLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLHHELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHTSGQSNGRDHQALAKAVQVHQDTLRTMYFA egfr_human Epidermal growth factor receptor ERBB1 EGFR Receptor tyrosine-protein kinase erbB-1 Proto-oncogene c-ErbB-1 ERBB HER1 protein protein human Homo sapiens Human MRPSGTAGAALLALLAALCPASRALEEKKVCQGTSNKLTQLGTFEDHFLSLQRMFNNCEVVLGNLEITYVQRNYDLSFLKTIQEVAGYVLIALNTVERIPLENLQIIRGNMYYENSYALAVLSNYDANKTGLKELPMRNLQEILHGAVRFSNNPALCNVESIQWRDIVSSDFLSNMSMDFQNHLGSCQKCDPSCPNGSCWGAGEENCQKLTKIICAQQCSGRCRGKSPSDCCHNQCAAGCTGPRESDCLVCRKFRDEATCKDTCPPLMLYNPTTYQMDVNPEGKYSFGATCVKKCPRNYVVTDHGSCVRACGADSYEMEEDGVRKCKKCEGPCRKVCNGIGIGEFKDSLSINATNIKHFKNCTSISGDLHILPVAFRGDSFTHTPPLDPQELDILKTVKEITGFLLIQAWPENRTDLHAFENLEIIRGRTKQHGQFSLAVVSLNITSLGLRSLKEISDGDVIISGNKNLCYANTINWKKLFGTSGQKTKIISNRGENSCKATGQVCHALCSPEGCWGPEPRDCVSCRNVSRGRECVDKCNLLEGEPREFVENSECIQCHPECLPQAMNITCTGRGPDNCIQCAHYIDGPHCVKTCPAGVMGENNTLVWKYADAGHVCHLCHPNCTYGCTGPGLEGCPTNGPKIPSIATGMVGALLLLLVVALGIGLFMRRRHIVRKRTLRRLLQERELVEPLTPSGEAPNQALLRILKETEFKKIKVLGSGAFGTVYKGLWIPEGEKVKIPVAIKELREATSPKANKEILDEAYVMASVDNPHVCRLLGICLTSTVQLITQLMPFGCLLDYVREHKDNIGSQYLLNWCVQIAKGMNYLEDRRLVHRDLAARNVLVKTPQHVKITDFGLAKLLGAEEKEYHAEGGKVPIKWMALESILHRIYTHQSDVWSYGVTVWELMTFGSKPYDGIPASEISSILEKGERLPQPPICTIDVYMIMVKCWMIDADSRPKFRELIIEFSKMARDPQRYLVIQGDERMHLPSPTDSNFYRALMDEEDMDDVVDADEYLIPQQGFFSSPSTSRTPLLSSLSATSNNSTVACIDRNGLQSCPIKEDSFLQRYSSDPTGALTEDSIDDTFLPVPEYINQSVPKRPAGSVQNPVYHNQPLNPAPSRDPHYQDPHSTAVGNPEYLNTVQPTCVNSTFDSPAHWAQKGSHQISLDNPDYQQDFFPKEAKPNGIFKGSTAENAEYLRVAPQSSEFIGA q9upy3-1 Endoribonuclease Dicer DICER1 DICER HERNA KIAA0928 Helicase with RNase motif protein protein human Homo sapiens Human MKSPALQPLSMAGLQLMTPASSPMGPFFGLPWQQEAIHDNIYTPRKYQVELLEAALDHNTIVCLNTGSGKTFIAVLLTKELSYQIRGDFSRNGKRTVFLVNSANQVAQQVSAVRTHSDLKVGEYSNLEVNASWTKERWNQEFTKHQVLIMTCYVALNVLKNGYLSLSDINLLVFDECHLAILDHPYREIMKLCENCPSCPRILGLTASILNGKCDPEELEEKIQKLEKILKSNAETATDLVVLDRYTSQPCEIVVDCGPFTDRSGLYERLLMELEEALNFINDCNISVHSKERDSTLISKQILSDCRAVLVVLGPWCADKVAGMMVRELQKYIKHEQEELHRKFLLFTDTFLRKIHALCEEHFSPASLDLKFVTPKVIKLLEILRKYKPYERQQFESVEWYNNRNQDNYVSWSDSEDDDEDEEIEEKEKPETNFPSPFTNILCGIIFVERRYTAVVLNRLIKEAGKQDPELAYISSNFITGHGIGKNQPRNKQMEAEFRKQEEVLRKFRAHETNLLIATSIVEEGVDIPKCNLVVRFDLPTEYRSYVQSKGRARAPISNYIMLADTDKIKSFEEDLKTYKAIEKILRNKCSKSVDTGETDIDPVMDDDDVFPPYVLRPDDGGPRVTINTAIGHINRYCARLPSDPFTHLAPKCRTRELPDGTFYSTLYLPINSPLRASIVGPPMSCVRLAERVVALICCEKLHKIGELDDHLMPVGKETVKYEEELDLHDEEETSVPGRPGSTKRRQCYPKAIPECLRDSYPRPDQPCYLYVIGMVLTTPLPDELNFRRRKLYPPEDTTRCFGILTAKPIPQIPHFPVYTRSGEVTISIELKKSGFMLSLQMLELITRLHQYIFSHILRLEKPALEFKPTDADSAYCVLPLNVVNDSSTLDIDFKFMEDIEKSEARIGIPSTKYTKETPFVFKLEDYQDAVIIPRYRNFDQPHRFYVADVYTDLTPLSKFPSPEYETFAEYYKTKYNLDLTNLNQPLLDVDHTSSRLNLLTPRHLNQKGKALPLSSAEKRKAKWESLQNKQILVPELCAIHPIPASLWRKAVCLPSILYRLHCLLTAEELRAQTASDAGVGVRSLPADFRYPNLDFGWKKSIDSKSFISISNSSSAENDNYCKHSTIVPENAAHQGANRTSSLENHDQMSVNCRTLLSESPGKLHVEVSADLTAINGLSYNQNLANGSYDLANRDFCQGNQLNYYKQEIPVQPTTSYSIQNLYSYENQPQPSDECTLLSNKYLDGNANKSTSDGSPVMAVMPGTTDTIQVLKGRMDSEQSPSIGYSSRTLGPNPGLILQALTLSNASDGFNLERLEMLGDSFLKHAITTYLFCTYPDAHEGRLSYMRSKKVSNCNLYRLGKKKGLPSRMVVSIFDPPVNWLPPGYVVNQDKSNTDKWEKDEMTKDCMLANGKLDEDYEEEDEEEESLMWRAPKEEADYEDDFLEYDQEHIRFIDNMLMGSGAFVKKISLSPFSTTDSAYEWKMPKKSSLGSMPFSSDFEDFDYSSWDAMCYLDPSKAVEEDDFVVGFWNPSEENCGVDTGKQSISYDLHTEQCIADKSIADCVEALLGCYLTSCGERAAQLFLCSLGLKVLPVIKRTDREKALCPTRENFNSQQKNLSVSCAAASVASSRSSVLKDSEYGCLKIPPRCMFDHPDADKTLNHLISGFENFEKKINYRFKNKAYLLQAFTHASYHYNTITDCYQRLEFLGDAILDYLITKHLYEDPRQHSPGVLTDLRSALVNNTIFASLAVKYDYHKYFKAVSPELFHVIDDFVQFQLEKNEMQGMDSELRRSEEDEEKEEDIEVPKAMGDIFESLAGAIYMDSGMSLETVWQVYYPMMRPLIEKFSANVPRSPVRELLEMEPETAKFSPAERTYDGKVRVTVEVVGKGKFKGVGRSYRIAKSAAARRALRSLKANQPQVPNS trbp2_human RISC-loading complex subunit TARBP2 Trans-activation-responsive RNA-binding protein TARBP2 TRBP TAR RNA-binding protein 2 protein protein human Homo sapiens Human MSEEEQGSGTTTGCGLPSIEQMLAANPGKTPISLLQEYGTRIGKTPVYDLLKAEGQAHQPNFTFRVTVGDTSCTGQGPSKKAAKHKAAEVALKHLKGGSMLEPALEDSSSFSPLDSSLPEDIPVFTAAAAATPVPSVVLTRSPPMELQPPVSPQQSECNPVGALQELVVQKGWRLPEYTVTQESGPAHRKEFTMTCRVERFIEIGSGTSKKLAKRNAAAKMLLRVHTVPLDARDGNEVEPDDDHFSIGVGSRLDGLRNRGPGCTWDSLRNSVGEKILSLRSCSLGSLGALGPACCRVLSELSEEQAFHVSYLDIEELSLSGLCQCLVELSTQPATVCHGSATTREAARGEAARRALQYLKIMAGSK egfr-ago2-1 3253735 N/A 3253749 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3253750 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false ago2-dicer1-19 3253736 N/A 3253749 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3253750 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3253751 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false ago2-egfr-1 3253737 N/A 3253749 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-517 Region 1-517 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3253750 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false in vitro In vitro direct interaction direct interaction false false false ago2-dicer1-20 3253738 N/A 3253749 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3253750 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3253751 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false egfr-ago2-2 3253739 N/A 3253749 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3253750 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association false false false ago2-egfr-2 3253740 N/A 3253749 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3253750 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false ago2-egfr-3 3253741 N/A 3253749 unspecified role unspecified role unspecified role unspecified role cYFP-tag cYFP-tag cyfp cYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3253750 unspecified role unspecified role unspecified role unspecified role nYFP-tag nYFP-tag nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human physical association physical association false false false ago2-dicer1-21 3253742 N/A 3253749 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3253752 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3253750 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human N/A 3253751 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false ago2-egfr-4 3253743 N/A 3253749 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3253750 unspecified role unspecified role prey prey P00533:p.Lys745Arg K745R mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false Region 669-1210 Region 669-1210 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human physical association physical association false false false egfr-13 3253744 N/A 3253750 putative self putative self unspecified role unspecified role pTyr pTyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false human Homo sapiens Human phosphorylation phosphorylation reaction phosphorylation false false false ago2-egfr-5 3253745 N/A 3253749 enzyme target enzyme target substrate substrate unspecified role unspecified role Q9UKV8:p.Tyr393Phe Y393F mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate substitut analysis substitution analysis substitut analysis certain certain sequence position certain certain certain sequence position certain false pTyr pTyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3253750 enzyme enzyme unspecified role unspecified role human Homo sapiens Human phosphorylation phosphorylation reaction phosphorylation false false false ago2-egfr-6 3253746 N/A 3253749 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false Region 1-372 Region 1-372 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false human Homo sapiens Human N/A 3253750 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range false human Homo sapiens Human physical association physical association false false false ago2-dicer1-22 3253747 N/A 3253749 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3253751 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association false false false ago2-dicer1-23 3253748 N/A 3253749 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range false human Homo sapiens Human N/A 3253752 unspecified role unspecified role prey prey human Homo sapiens Human N/A 3253751 unspecified role unspecified role prey prey human Homo sapiens Human association association false false false