DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ mocibob-2013-1 in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. DIP original experiment: DIP-252249X Structure (London, England : 1993) 2013 IMEx Adaptation of aminoacyl-tRNA synthetase catalytic core to carrier protein aminoacylation. Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. Only protein-protein interactions imex curation mocibob-2013-2 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. DIP original experiment: DIP-252241X Structure (London, England : 1993) 2013 IMEx Adaptation of aminoacyl-tRNA synthetase catalytic core to carrier protein aminoacylation. Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. Only protein-protein interactions imex curation mocibob-2013-3 in vitro In vitro aminoacylation aminoacylation assay aminoacylation predetermined predetermined participant predetermined Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. DIP original experiment: DIP-252244X Structure (London, England : 1993) 2013 IMEx Adaptation of aminoacyl-tRNA synthetase catalytic core to carrier protein aminoacylation. Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. Only protein-protein interactions imex curation mocibob-2013-4 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. DIP original experiment: DIP-252242X Structure (London, England : 1993) 2013 IMEx Adaptation of aminoacyl-tRNA synthetase catalytic core to carrier protein aminoacylation. Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. Only protein-protein interactions imex curation mocibob-2013-5 in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. DIP original experiment: DIP-252247X Structure (London, England : 1993) 2013 IMEx Adaptation of aminoacyl-tRNA synthetase catalytic core to carrier protein aminoacylation. Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. Only protein-protein interactions imex curation mocibob-2013-6 in vitro In vitro aminoacylation aminoacylation assay aminoacylation predetermined predetermined participant predetermined Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. DIP original experiment: DIP-252245X Structure (London, England : 1993) 2013 IMEx Adaptation of aminoacyl-tRNA synthetase catalytic core to carrier protein aminoacylation. Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. Only protein-protein interactions imex curation mocibob-2013-7 in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. DIP original experiment: DIP-252243X Structure (London, England : 1993) 2013 IMEx Adaptation of aminoacyl-tRNA synthetase catalytic core to carrier protein aminoacylation. Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. Only protein-protein interactions imex curation mocibob-2013-8 in vitro In vitro aminoacylation aminoacylation assay aminoacylation predetermined predetermined participant predetermined Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. DIP original experiment: DIP-252246X Structure (London, England : 1993) 2013 IMEx Adaptation of aminoacyl-tRNA synthetase catalytic core to carrier protein aminoacylation. Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. Only protein-protein interactions imex curation mocibob-2013-9 in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. DIP original experiment: DIP-252248X Structure (London, England : 1993) 2013 IMEx Adaptation of aminoacyl-tRNA synthetase catalytic core to carrier protein aminoacylation. Mocibob M., Ivic N., Luic M., Weygand-Durasevic I. Only protein-protein interactions imex curation aacp1_bradu Aminoacyl carrier protein 1 bsr0959 protein protein braja Bradyrhizobium japonicum (strain USDA 110) MQAFNTDVRNRIIKLVKGILEQNALAADVTPQAKLVDVGLTSMDMVNLMLGVEAEFDFTIPQSEITPENFQSVETLERMVMTQLQPATAA aacl1_bradu Amino acid--[acyl-carrier-protein] ligase 1 Amino acid:[carrier-protein] ligase [AMP forming] 1 Aminoacyl-[acyl-carrier-protein] synthetase 1 L-glycine:[acyl-carrier-protein] ligase 1 bll0957 protein protein braja Bradyrhizobium japonicum (strain USDA 110) MNIAVLPNSPDTAPQIADPLDHLADKLFHSMGSDGVYARTALYESIVERLAALITSHREAGTEALRFPPVMSRAQLEKSGYLKSFPNLLGCVCGLHGTEREINAAVSRFDAGGDWTTSLSPADLVLSPAACYPVYPIAASRGPLPKGGLRFDVAADCFRREPSKHLDRLQSFRMREYVCIGTPDDVSDFRERWMVRAQAIARDLGLTFRVDYASDPFFGRVGQMKAVSQKQQQLKFELLIPLRSEEQPTACMSFNYHREHFGTTWGIQDANGEPAHTGCVAFGMDRLAVAMFHTHGTDLSAWPAKVRDILGLQPHVAAGAHGEGWR aacp_agrfc Aminoacyl carrier protein AGR_C_4658 Atu2571 protein protein agrt5 Agrobacterium tumefaciens (strain C58 / ATCC 33970) Agrobacterium tumefaciens (strain C58) MNATIREILAKFGQLPTPVDTIADEADLYAAGLSSFASVQLMLGIEEAFDIEFPDNLLNRKSFASIKAIEDTVKLILDGKEAA aacl_agrfc Amino acid--[acyl-carrier-protein] ligase Amino acid:[carrier-protein] ligase [AMP forming] Aminoacyl-[acyl-carrier-protein] synthetase L-alanine:[acyl-carrier-protein] ligase L-glycine:[acyl-carrier-protein] ligase L-serine:[acyl-carrier-protein] ligase AGR_C_4663 Atu2573 protein protein agrt5 Agrobacterium tumefaciens (strain C58 / ATCC 33970) Agrobacterium tumefaciens (strain C58) MTVFSAIPPISCWFTGRTPASWDKTMDMQTSFLDRLFEEGLLIETGVDGLYGRSGQFEDVIAAFERLIDRTGGADGAEAIRFPPGINRAYFEKSGYMKSFPQLAGTVHSFCGCELDHVSLLKSMDEGGDWTKDQKATDIVLTPAACYPLYPTIAKRGALPAGGGLYDIQSYCFRHEPSKDPARQQLFRMREYVCMGTESDVTEFRQTWMDRGVEMMKAVGLDVTIDIANDPFFGRAGKMLANNQRDQNLKFELLIPVTSATNPTACMSFNYHQDAFGQKWGLNLENGDVAHTACVGFGLERIALALFAHHGLDVKKWPAKVVETLWG aacp1_bradu-aacl1_bradu-1 3252480 N/A 3252489 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3252490 unspecified role unspecified role prey prey Region 214-219 Region 214-219 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli direct interaction direct interaction false false false aacl1_bradu-aacp1_bradu-1 3252481 N/A 3252489 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3252490 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false aacl1_bradu-aacp1_bradu-2 3252482 N/A 3252489 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli N/A 3252490 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli aminoacylation aminoacylation reaction aminoacylation false false false aacl1_bradu-aacp1_bradu-3 3252483 N/A 3252489 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3252490 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false aacp_agrfc-aacl_agrfc-1 3252484 N/A 3252491 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3252492 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false aacl_agrfc-aacp_agrfc-1 3252485 N/A 3252491 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli N/A 3252492 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli aminoacylation aminoacylation reaction aminoacylation false false false aacl_agrfc-aacp_agrfc-2 3252486 N/A 3252491 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli N/A 3252492 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction false false false aacl_agrfc-aacp1_bradu-1 3252487 N/A 3252489 enzyme target enzyme target substrate substrate unspecified role unspecified role ecolx Escherichia coli N/A 3252492 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli aminoacylation aminoacylation reaction aminoacylation false false false aacp1_bradu-aacl_agrfc-1 3252488 N/A 3252489 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false ecolx Escherichia coli N/A 3252492 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction false false false