IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ http://www.ebi.ac.uk/ yuan-2013-1 yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast ub reconstruction ubiquitin reconstruction ub reconstruction predetermined predetermined participant predetermined Yuan L., Gu R., Xuan Y., Smith-Valle E., Loqué D., Frommer WB., von Wirén N. vonwiren@ipk-gatersleben.de The Plant cell 2013 imex curation Only protein-protein interactions imex curation yuan-2013-2 nicbe-leaf Nicotiana benthamiana leaf leaf leaf bifc bimolecular fluorescence complementation bifc tag fluorescence tag visualisation by fluorescence tag fluorescence Yuan L., Gu R., Xuan Y., Smith-Valle E., Loqué D., Frommer WB., von Wirén N. Reconstitution of YFP fluorescence was carried out by using nYFP and cCFP fused proteins. vonwiren@ipk-gatersleben.de The Plant cell 2013 imex curation Only protein-protein interactions imex curation amt11_arath Ammonium transporter 1 member 1 AMT1-1 T6G15.60 At4g13510 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSCSATDLAVLLGPNATAAANYICGQLGDVNNKFIDTAFAIDNTYLLFSAYLVFSMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGSPSNGFIGKHYFGLKDIPTASADYSNFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFVYPVVSHWFWSVDGWASPFRTDGDLLFSTGAIDFAGSGVVHMVGGIAGLWGALIEGPRLGRFDNGGRAIALRGHSASLVVLGTFLLWFGWYGFNPGSFNKILVTYETGTYNGQWSAVGRTAVTTTLAGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITGGCSVVEPWAAIICGFVAALVLLGCNKLAEKLKYDDPLEAAQLHGGCGAWGLIFTALFAQEKYLNQIYGNKPGRPHGLFMGGGGKLLGAQLIQIIVITGWVSATMGTLFFILKKMKLLRISSEDEMAGMDMTRHGGFAYMYFDDDESHKAIQLRRVEPRSPSPSGANTTPTPV amt13_arath Ammonium transporter 1 member 3 AMT1-3 K7M2.7 At3g24300 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MSGAITCSAADLATLLGPNATAAADYICGQLGTVNNKFTDAAFAIDNTYLLFSAYLVFAMQLGFAMLCAGSVRAKNTMNIMLTNVLDAAAGGLFYYLFGYAFAFGGSSEGFIGRHNFALRDFPTPTADYSFFLYQWAFAIAAAGITSGSIAERTQFVAYLIYSSFLTGFVYPVVSHWFWSPDGWASPFRSADDRLFSTGAIDFAGSGVVHMVGGIAGLWGALIEGPRRGRFEKGGRAIALRGHSASLVVLGTFLLWFGWYGFNPGSFTKILVPYNSGSNYGQWSGIGRTAVNTTLSGCTAALTTLFGKRLLSGHWNVTDVCNGLLGGFAAITAGCSVVEPWAAIVCGFMASVVLIGCNKLAELVQYDDPLEAAQLHGGCGAWGLIFVGLFAKEKYLNEVYGATPGRPYGLFMGGGGKLLGAQLVQILVIVGWVSATMGTLFFILKRLNLLRISEQHEMQGMDMTRHGGFAYIYHDNDDESHRVDPGSPFPRSATPPRV amt1_1-amt1_1-1 3322577 N/A 3322579 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c_terminus cub cub c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3322579 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c_terminus nub nub c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 2 amt1_1-amt1_1-2 3322577 N/A 3322580 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c_terminus nub nub c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3322579 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c_terminus cub cub c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 2-2 amt1_1-amt1_3-1 3322577 N/A 3322580 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c_terminus nub nub c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3322580 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c_terminus cub cub c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 2-3 amt1_3-amt1_3-1 3322577 N/A 3322579 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed c_terminus nub nub c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3322580 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed c_terminus cub cub c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Figure 2-4 amt1_1-amt1_1-3 3322578 N/A 3322579 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c_terminus nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3322579 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c_terminus cgfp cGFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Supp. Figure 9 amt1_1-amt1_3-2 3322578 N/A 3322580 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c_terminus cgfp cGFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3322579 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c_terminus nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Supp Figure 9 amt1_3-amt1_3-2 3322578 N/A 3322580 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c_terminus cgfp cGFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false N/A 3322580 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed c_terminus nyfp nYFP c-term range C-terminal range c-term range c-term range C-terminal range c-term range false physical association physical association false false false Supp. Figure 9