DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ nance-2013-1 in vitro In vitro pull down pull down tag fluorescence tag visualisation by fluorescence tag fluorescence Nance MR., Kreutz B., Tesmer VM., Sterne-Marr R., Kozasa T., Tesmer JJ. DIP original experiment: DIP-252173X Structure (London, England : 1993) 2013 IMEx Structural and functional analysis of the regulator of G protein signaling 2-gαq complex. Nance MR., Kreutz B., Tesmer VM., Sterne-Marr R., Kozasa T., Tesmer JJ. Only protein-protein interactions imex curation nance-2013-2 in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Nance MR., Kreutz B., Tesmer VM., Sterne-Marr R., Kozasa T., Tesmer JJ. DIP original experiment: DIP-252171X Structure (London, England : 1993) 2013 IMEx Structural and functional analysis of the regulator of G protein signaling 2-gαq complex. Nance MR., Kreutz B., Tesmer VM., Sterne-Marr R., Kozasa T., Tesmer JJ. Only protein-protein interactions imex curation nance-2013-3 in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Nance MR., Kreutz B., Tesmer VM., Sterne-Marr R., Kozasa T., Tesmer JJ. DIP original experiment: DIP-252172X Structure (London, England : 1993) 2013 IMEx Structural and functional analysis of the regulator of G protein signaling 2-gαq complex. Nance MR., Kreutz B., Tesmer VM., Sterne-Marr R., Kozasa T., Tesmer JJ. Only protein-protein interactions imex curation p41220-1 Regulator of G-protein signaling 2 RGS2 G0S8 GIG31 Cell growth-inhibiting gene 31 protein G0/G1 switch regulatory protein 8 protein protein human Homo sapiens Human MQSAMFLAVQHDCRPMDKSAGSGHKSEEKREKMKRTLLKDWKTRLSYFLQNSSTPGKPKTGKKSKQQAFIKPSPEEAQLWSEAFDELLASKYGLAAFRAFLKSEFCEENIEFWLACEDFKKTKSPQKLSSKARKIYTDFIEKEAPKEINIDFQTKTLIAQNIQEATSGCFTTAQKRVYSLMENNSYPRFLESEFYQDLCKKPQITTEPHAT gnaq_mouse Guanine nucleotide-binding protein G(q) subunit alpha Guanine nucleotide-binding protein alpha-q Gnaq protein protein mouse Mus musculus Mouse MTLESIMACCLSEEAKEARRINDEIERQLRRDKRDARRELKLLLLGTGESGKSTFIKQMRIIHGSGYSDEDKRGFTKLVYQNIFTAMQAMIRAMDTLKIPYKYEHNKAHAQLVREVDVEKVSAFENPYVDAIKSLWNDPGIQECYDRRREYQLSDSTKYYLNDLDRVADPSYLPTQQDVLRVRVPTTGIIEYPFDLQSVIFRMVDVGGQRSERRKWIHCFENVTSIMFLVALSEYDQVLVESDNENRMEESKALFRTIITYPWFQNSSVILFLNKKDLLEEKIMYSHLVDYFPEYDGPQRDAQAAREFILKMFVDLNPDSDKIIYSHFTCATDTENIRFVFAAVKDTILQLNLKEYNLV gnaq-rgs2-1 3251094 N/A 3251097 unspecified role unspecified role prey prey Alexa488-label Alexa488-label alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined false ecolx Escherichia coli N/A 3251098 unspecified role unspecified role bait bait Region 7-359 Region 7-359 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false gnaq-rgs2-2 3251095 N/A 3251097 unspecified role unspecified role unspecified role unspecified role Region 72-203 Region 72-203 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3251098 unspecified role unspecified role unspecified role unspecified role Region 18-359 Region 18-359 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false gnaq-rgs2-3 3251096 N/A 3251097 unspecified role unspecified role unspecified role unspecified role Region 72-203 Region 72-203 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false ecolx Escherichia coli N/A 3251098 unspecified role unspecified role unspecified role unspecified role Region 18-359 Region 18-359 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain false trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction false false false